Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri

Alpha thalassemia is a common recessively inherited blood disorder due to mutation or deletion of one or more alpha globin gene. Nowadays, the initial step for screening of alpha thalassemia is by determining the MCV and MCH values. Further investigation includes Hb analysis and confirmation test by...

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Main Author:	Ahmad Basri, Ahmad Izzat
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Examination of the blood
Online Access:	https://ir.uitm.edu.my/id/eprint/19407/3/19407.pdf
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id	my-uitm-ir.19407
record_format	uketd_dc
spelling	my-uitm-ir.194072024-11-25T01:16:22Z Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri 2016 Ahmad Basri, Ahmad Izzat Examination of the blood Alpha thalassemia is a common recessively inherited blood disorder due to mutation or deletion of one or more alpha globin gene. Nowadays, the initial step for screening of alpha thalassemia is by determining the MCV and MCH values. Further investigation includes Hb analysis and confirmation test by molecular analysis. Among the popular molecular method commonly used today is the polymerase chain reaction (PCR). HTAR usually outsource its molecular tests for alpha thalassemia. However, only about 50% of the outsourced samples were positive for alpha thalassemia. Thus, to avoid performing molecular analysis unnecessarily, this study was proposed to find if there is any relationship between the RBC parameters, HBA₂ level and molecular findings for better screening of patients prior to request for molecular analysis. A total of 197 data which consisted of RBC parameters (TRBC, Hb, RDW, MCV, MCH, MCHC and Retic Count), HBA₂ level and molecular findings from HTAR patients diagnosed with alpha thalassemia in 2014. Independent t-test was conducted to evaluate whether the RBC parameters and , HBA₂ level differed significantly for groups of positive or negative molecular findings. Out of the eight parameters tested, RDW, MCHC and rectic were found to be significantly different between groups of positive and negative molecular analysis. Then, the RDW, MCHC and Retic were then anaylzed using ROC Curve to determine the cut-off values for positive molecular findings, respectively. The cut-off values for MCHC and Retic could be determined while cut-off values for RDW could not be determined as the area under the curve was less than 0.5. The cut-off values for MCHC was equal or greater than 34.15 g/dL while the cut-off value for Retic was equal or greater than 2.1%. 2016 Thesis https://ir.uitm.edu.my/id/eprint/19407/ https://ir.uitm.edu.my/id/eprint/19407/3/19407.pdf text en public other degree Universiti Teknologi MARA, Selangor Faculty of Health Science
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Examination of the blood
spellingShingle	Examination of the blood Ahmad Basri, Ahmad Izzat Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri
description	Alpha thalassemia is a common recessively inherited blood disorder due to mutation or deletion of one or more alpha globin gene. Nowadays, the initial step for screening of alpha thalassemia is by determining the MCV and MCH values. Further investigation includes Hb analysis and confirmation test by molecular analysis. Among the popular molecular method commonly used today is the polymerase chain reaction (PCR). HTAR usually outsource its molecular tests for alpha thalassemia. However, only about 50% of the outsourced samples were positive for alpha thalassemia. Thus, to avoid performing molecular analysis unnecessarily, this study was proposed to find if there is any relationship between the RBC parameters, HBA₂ level and molecular findings for better screening of patients prior to request for molecular analysis. A total of 197 data which consisted of RBC parameters (TRBC, Hb, RDW, MCV, MCH, MCHC and Retic Count), HBA₂ level and molecular findings from HTAR patients diagnosed with alpha thalassemia in 2014. Independent t-test was conducted to evaluate whether the RBC parameters and , HBA₂ level differed significantly for groups of positive or negative molecular findings. Out of the eight parameters tested, RDW, MCHC and rectic were found to be significantly different between groups of positive and negative molecular analysis. Then, the RDW, MCHC and Retic were then anaylzed using ROC Curve to determine the cut-off values for positive molecular findings, respectively. The cut-off values for MCHC and Retic could be determined while cut-off values for RDW could not be determined as the area under the curve was less than 0.5. The cut-off values for MCHC was equal or greater than 34.15 g/dL while the cut-off value for Retic was equal or greater than 2.1%.
format	Thesis
qualification_name	other
qualification_level	Bachelor degree
author	Ahmad Basri, Ahmad Izzat
author_facet	Ahmad Basri, Ahmad Izzat
author_sort	Ahmad Basri, Ahmad Izzat
title	Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri
title_short	Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri
title_full	Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri
title_fullStr	Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri
title_full_unstemmed	Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri
title_sort	relationship between rbc parameters, hba₂ level and molecular findings in alpha thalassemia: htar experience / ahmad izzat ahmad basri
granting_institution	Universiti Teknologi MARA, Selangor
granting_department	Faculty of Health Science
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/19407/3/19407.pdf
_version_	1818587847623442432

Relationship between RBC parameters, HBA₂ level and molecular findings in Alpha Thalassemia: HTAR experience / Ahmad Izzat Ahmad Basri

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