Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac

Gynura procumbens, or also known as “Sambung nyawa” is a type of herbs that is widely used in South East Asian countries, particularly in Indonesia, Malaysia and Thailand. It is traditionally used for treatment of many diseases such as cancer, kidney diseases, migraine, hypertension and diabetes. Th...

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Main Author:	Isaac, Adderly Izna
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Medical education Medical schools Research Medicinal plants (Culture only)
Online Access:	https://ir.uitm.edu.my/id/eprint/19955/1/TD_%20ADDERLY%20IZNA%20ISAAC%20%20HC%20B%2016_5.pdf
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spelling	my-uitm-ir.199552018-03-21T04:05:36Z Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac 2016 Isaac, Adderly Izna Medical education. Medical schools. Research Medicinal plants (Culture only) Gynura procumbens, or also known as “Sambung nyawa” is a type of herbs that is widely used in South East Asian countries, particularly in Indonesia, Malaysia and Thailand. It is traditionally used for treatment of many diseases such as cancer, kidney diseases, migraine, hypertension and diabetes. The present study was conducted to investigate the cytotoxicity of peripheral blood mononuclear cells (PBMCs) treated with Gynura procumbens extract. PBMCs were separated from whole blood, and 2 x 105 cells/well were cultured and treated with a 0.0 μg/ml, 6.25 μg/ml, 12.5 μg/ml, 25.0 μg/ml, 50.0 μg/ml, 100.0 μg/ml, 200.0 μg/ml, 400.0 μg/ml, 800.0 μg/ml and 1600.0 μg/ml of aqueous and ethanolic extract of Gynura procumbens for a period of 24 hours. 10 μg/ml of lipopolysaccharide(LPS) from Esherichia coli 0157, was treated onto the positive control wells. Result shows that Gynura procumbens extracts, both aqueous and ethanolic, does not cause any significant cytotoxicity effect on PBMC. Most concentrations show more than 80% of the percentage of living cell, thus indicating that concentration ranging from 0.0 μg/mL until 1600.0 μg/mL is safe to be used. The comparison of cytotoxicity effect between aqueous and ethanolic extract also does not give any significant differences. Further study of cytotoxicity of this plant using higher concentration is recommended. 2016 Thesis https://ir.uitm.edu.my/id/eprint/19955/ https://ir.uitm.edu.my/id/eprint/19955/1/TD_%20ADDERLY%20IZNA%20ISAAC%20%20HC%20B%2016_5.pdf text en public other degree Universiti teknologi MARA, Selangor Faculty of Health Science
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Medical education Medical schools Research Medicinal plants (Culture only)
spellingShingle	Medical education Medical schools Research Medicinal plants (Culture only) Isaac, Adderly Izna Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac
description	Gynura procumbens, or also known as “Sambung nyawa” is a type of herbs that is widely used in South East Asian countries, particularly in Indonesia, Malaysia and Thailand. It is traditionally used for treatment of many diseases such as cancer, kidney diseases, migraine, hypertension and diabetes. The present study was conducted to investigate the cytotoxicity of peripheral blood mononuclear cells (PBMCs) treated with Gynura procumbens extract. PBMCs were separated from whole blood, and 2 x 105 cells/well were cultured and treated with a 0.0 μg/ml, 6.25 μg/ml, 12.5 μg/ml, 25.0 μg/ml, 50.0 μg/ml, 100.0 μg/ml, 200.0 μg/ml, 400.0 μg/ml, 800.0 μg/ml and 1600.0 μg/ml of aqueous and ethanolic extract of Gynura procumbens for a period of 24 hours. 10 μg/ml of lipopolysaccharide(LPS) from Esherichia coli 0157, was treated onto the positive control wells. Result shows that Gynura procumbens extracts, both aqueous and ethanolic, does not cause any significant cytotoxicity effect on PBMC. Most concentrations show more than 80% of the percentage of living cell, thus indicating that concentration ranging from 0.0 μg/mL until 1600.0 μg/mL is safe to be used. The comparison of cytotoxicity effect between aqueous and ethanolic extract also does not give any significant differences. Further study of cytotoxicity of this plant using higher concentration is recommended.
format	Thesis
qualification_name	other
qualification_level	Bachelor degree
author	Isaac, Adderly Izna
author_facet	Isaac, Adderly Izna
author_sort	Isaac, Adderly Izna
title	Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac
title_short	Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac
title_full	Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac
title_fullStr	Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac
title_full_unstemmed	Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac
title_sort	cytotoxicity assay of peripheral blood mononuclear cells (pbmc) treated with gynura procumbens extract / adderly izna isaac
granting_institution	Universiti teknologi MARA, Selangor
granting_department	Faculty of Health Science
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/19955/1/TD_%20ADDERLY%20IZNA%20ISAAC%20%20HC%20B%2016_5.pdf
_version_	1783733722931003392

Cytotoxicity Assay of Peripheral Blood Mononuclear Cells (Pbmc) treated with Gynura Procumbens extract / Adderly Izna Isaac

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