Microbial transformation of steroid / Balqis Haya Ismail
Microbial transformation by endophytes was studied since centuries ago. This phenomenon allows a compound to be modified into another compound by using green synthesis instead of chemical synthesis. In this study, Mesterolone as the substrate undergoes fermentation with identified Aspergillus SSW...
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2010
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my-uitm-ir.22072016-10-11T08:15:58Z Microbial transformation of steroid / Balqis Haya Ismail 2010 Ismail, Balqis Haya RM Therapeutics. Pharmacology Microbial transformation by endophytes was studied since centuries ago. This phenomenon allows a compound to be modified into another compound by using green synthesis instead of chemical synthesis. In this study, Mesterolone as the substrate undergoes fermentation with identified Aspergillus SSW and 11L2. Outline of method involved are media preparation, inoculation of fungi, feeding of substrate, incubation for 6 or 12 days and extraction using ethyl acetate. The extracts were analyzed using TLC and HPLC to indicate the biotransformed products. Besides that, each product extracted was also tested for antimicrobial activity by disk diffusion assay, which include activity against bacteria of E. Coli and B. Subtilis as well as fungi represented by A. Niger. Products only by SSW show potential antimicrobial activity. Further study should be done to purify and identify resulting compound Faculty of Pharmacy 2010 Thesis https://ir.uitm.edu.my/id/eprint/2207/ https://ir.uitm.edu.my/id/eprint/2207/1/TD_BALQIS%20HAYA%20ISMAIL%2010_5%201.pdf text en public degree Universiti Teknologi MARA Faculty of Pharmacy |
| institution |
Universiti Teknologi MARA |
| collection |
UiTM Institutional Repository |
| language |
English |
| topic |
RM Therapeutics Pharmacology |
| spellingShingle |
RM Therapeutics Pharmacology Ismail, Balqis Haya Microbial transformation of steroid / Balqis Haya Ismail |
| description |
Microbial transformation by endophytes was studied since centuries ago. This
phenomenon allows a compound to be modified into another compound by using
green synthesis instead of chemical synthesis. In this study, Mesterolone as the
substrate undergoes fermentation with identified Aspergillus SSW and 11L2. Outline
of method involved are media preparation, inoculation of fungi, feeding of substrate,
incubation for 6 or 12 days and extraction using ethyl acetate. The extracts were
analyzed using TLC and HPLC to indicate the biotransformed products. Besides that,
each product extracted was also tested for antimicrobial activity by disk diffusion
assay, which include activity against bacteria of E. Coli and B. Subtilis as well as
fungi represented by A. Niger. Products only by SSW show potential antimicrobial
activity. Further study should be done to purify and identify resulting compound |
| format |
Thesis |
| qualification_level |
Bachelor degree |
| author |
Ismail, Balqis Haya |
| author_facet |
Ismail, Balqis Haya |
| author_sort |
Ismail, Balqis Haya |
| title |
Microbial transformation of steroid / Balqis Haya Ismail |
| title_short |
Microbial transformation of steroid / Balqis Haya Ismail |
| title_full |
Microbial transformation of steroid / Balqis Haya Ismail |
| title_fullStr |
Microbial transformation of steroid / Balqis Haya Ismail |
| title_full_unstemmed |
Microbial transformation of steroid / Balqis Haya Ismail |
| title_sort |
microbial transformation of steroid / balqis haya ismail |
| granting_institution |
Universiti Teknologi MARA |
| granting_department |
Faculty of Pharmacy |
| publishDate |
2010 |
| url |
https://ir.uitm.edu.my/id/eprint/2207/1/TD_BALQIS%20HAYA%20ISMAIL%2010_5%201.pdf |
| _version_ |
1783733069190004736 |