Effect of different drying method on antioxidant capacity and total flavonoid contents of Artocarpus Heterophyllus Lam. (Jackfruit) leaf extract / Abdul Muqit Abdul Waled@Suhaimi
Artocarpus spp was identified to have numerous biological activity through an in vitro and in vivo pharmacological studies and these elucidated by the phenolic compounds such as flavonoids, stilbenoids, arylbenzofurons that present in different of plant parts. Mainly in the actual studies and review...
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| Format: | Thesis |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/22288/1/TD_ABDUL%20MUQIT%20ABDUL%20WALED%20HS%20B%2015_5.pdf |
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| Summary: | Artocarpus spp was identified to have numerous biological activity through an in vitro and in vivo pharmacological studies and these elucidated by the phenolic compounds such as flavonoids, stilbenoids, arylbenzofurons that present in different of plant parts. Mainly in the actual studies and reviews on the antioxidant capability of natural product focus more on the characteristics of the
measurement procedure for instance free radical generating system, redox process and hydrophilic solubility. Nevertheless, the vital steps such as preparation of sample or the procedure for extraction of antioxidants compound were not concentrated critically. Thus far, less attention in systematic analysis of the effects of different drying methods on the total flavonoid content and
antioxidant activity of Artocarpus heterophyllus Lam (jackfruit) leaf extract. This study is aim to determine the effects of different drying methods on the phenolic and flavonoid content and the antioxidant capacity of Artocarpus heterophyllus Lam (jackfruit) leaf aqueous and ethanolic extract. Jackfruit leaves were subjected to three different drying methods such as, sun drying, oven drying and air drying. The weight loss of the oven-dried, sun-dried and airdried samples will be recorded at time intervals and the drying experiments will be conducted in triplicate. Drying process were be stop when no significant changes in three consecutive weight. 10% aqueous and 70% ethanol extraction was chosen in these experiments to obtain a crude extract of freeze dry powder and dark-green viscous mass crude extract from rotary evaporation. Analysis of
total phenolic and flavonoid contents as well as its antioxidant capacity and ferric reducing power of jackfruit leaf extract were then determined. The total phenolic content (TPC) of ethanolic and aqueous extracts was significantly different as affected by different drying treatments, with air dried and oven dried produce slightly decrease in TPC and antioxidant activity as demonstrated by the reduction in both free radical scavenging activity of DPPH and lowering the FRAP values. Oven dried extract causing the highest decrease in TPC and antioxidant activity. However, sun dried jackfruit leaves extracts possessed a high TPC and antioxidant activity compared to the air and oven drying hence suggesting that sun dried methods is the most preferable method for processing
Artocarpus heterophyllus Lam (jackfruit) leaves in order to preserve the antioxidant properties, phenolic content as well as total flavonoids content. |
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