Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli

Petroleum refineries generate huge volumes of petroleum sludge during the process of refining crude oil. It is well known that petroleum sludge contains toxic, mutagenic and carcinogenic compounds that constitute hazard to human and the environme nt. Applications of bioremediation in not a new metho...

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Main Author:	Dzulkafli, Noor Fazreen
Format:	Thesis
Language:	English
Published:	2007
Subjects:	Petroleum refining Petroleum products
Online Access:	https://ir.uitm.edu.my/id/eprint/27210/2/27210.pdf
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spelling	my-uitm-ir.272102023-04-07T04:21:47Z Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli 2007 Dzulkafli, Noor Fazreen Petroleum refining. Petroleum products Petroleum refineries generate huge volumes of petroleum sludge during the process of refining crude oil. It is well known that petroleum sludge contains toxic, mutagenic and carcinogenic compounds that constitute hazard to human and the environme nt. Applications of bioremediation in not a new method for treatment of petroleum hydrocarbons contaminations. With advances in biotechnology, bioremediation has become one of the most rapidly developing fields of environmental restoration. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi and yeast. However, bacteria play the central role in hydrocarbon degradat ion. The driving force for petroleum biodegradation is the ability of microorganisms to utilize hydrocarbons to satisfy their cell growth and energy needs. Studies on the microbial processes to degrade hydrocarbon from several countries in arid region to date have established information on the best consortium of microorganisms to be used. However, very few reports are available on studies in tropical countries like Malaysia. Thus, this study investigates microbial species present in petroleum sludge that are capable of degrading hydrocarbon. Hydrocarbons used in this study include low chain hydrocarbon; n-decane, tetradecane, pentadecane and do-decane and also PAH namely; phenathrane, anthracene and dibenzothiophene. A series of experiments were conducted to enrich and isolates bacteria strains that are capable of degrading the hydrocarbons. These were followed by hydrocarbon degradation test and identification of bacteria strains that are capable of degrading hydrocarbon. Hydrocarbon degradation tests were done by using microtiter plate technique with INT as the indicator. A total of 53 strains capab le of degrading hydrocarbon were successfully isolated. Three of best strains were selected to be identified by using biochemical test. The best active strains were selected base on their ability to degrade PAH compound namely; phenathrane, anthracene and dibenzothiophene. These three strains were identified as Clavibacter michiganesis ss insidiosus, Brevibacterium otitidis and Rhodococcus rhodochrus. 2007 Thesis https://ir.uitm.edu.my/id/eprint/27210/ https://ir.uitm.edu.my/id/eprint/27210/2/27210.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Civil Engineering Abdul Talib, Suhaimi Ramasamy, Kalavathy
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Talib, Suhaimi Ramasamy, Kalavathy
topic	Petroleum refining Petroleum products
spellingShingle	Petroleum refining Petroleum products Dzulkafli, Noor Fazreen Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli
description	Petroleum refineries generate huge volumes of petroleum sludge during the process of refining crude oil. It is well known that petroleum sludge contains toxic, mutagenic and carcinogenic compounds that constitute hazard to human and the environme nt. Applications of bioremediation in not a new method for treatment of petroleum hydrocarbons contaminations. With advances in biotechnology, bioremediation has become one of the most rapidly developing fields of environmental restoration. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi and yeast. However, bacteria play the central role in hydrocarbon degradat ion. The driving force for petroleum biodegradation is the ability of microorganisms to utilize hydrocarbons to satisfy their cell growth and energy needs. Studies on the microbial processes to degrade hydrocarbon from several countries in arid region to date have established information on the best consortium of microorganisms to be used. However, very few reports are available on studies in tropical countries like Malaysia. Thus, this study investigates microbial species present in petroleum sludge that are capable of degrading hydrocarbon. Hydrocarbons used in this study include low chain hydrocarbon; n-decane, tetradecane, pentadecane and do-decane and also PAH namely; phenathrane, anthracene and dibenzothiophene. A series of experiments were conducted to enrich and isolates bacteria strains that are capable of degrading the hydrocarbons. These were followed by hydrocarbon degradation test and identification of bacteria strains that are capable of degrading hydrocarbon. Hydrocarbon degradation tests were done by using microtiter plate technique with INT as the indicator. A total of 53 strains capab le of degrading hydrocarbon were successfully isolated. Three of best strains were selected to be identified by using biochemical test. The best active strains were selected base on their ability to degrade PAH compound namely; phenathrane, anthracene and dibenzothiophene. These three strains were identified as Clavibacter michiganesis ss insidiosus, Brevibacterium otitidis and Rhodococcus rhodochrus.
format	Thesis
qualification_level	Master's degree
author	Dzulkafli, Noor Fazreen
author_facet	Dzulkafli, Noor Fazreen
author_sort	Dzulkafli, Noor Fazreen
title	Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli
title_short	Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli
title_full	Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli
title_fullStr	Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli
title_full_unstemmed	Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli
title_sort	bioremediation of petroleum sludge from the exxon mobil petroleum refining plant at kerteh, malaysia / noor fazreen dzulkafli
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Civil Engineering
publishDate	2007
url	https://ir.uitm.edu.my/id/eprint/27210/2/27210.pdf
_version_	1783733937940463616

Bioremediation of petroleum sludge from the Exxon Mobil petroleum refining plant at Kerteh, Malaysia / Noor Fazreen Dzulkafli

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