Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat

Pasteurella multocida (P. multocida) serotype B is associated with hemorrhagic septicaemia (HS) disease endemic in Africa, India and Asian countries. It is causative agent of thriftily significant disease in livestock. Hence, this study purposed and aimed to identify and express an immunogenic solub...

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Main Author:	Jumahat, Noor Masyitah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	RM Therapeutics Pharmacology
Online Access:	https://ir.uitm.edu.my/id/eprint/27214/1/TM_NOOR%20MASYITAH%20JUMAHAT%20MD%2015_5.pdf
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spelling	my-uitm-ir.272142020-01-14T02:07:13Z Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat 2015-04 Jumahat, Noor Masyitah RM Therapeutics. Pharmacology Immunotherapy. Serotherapy Pasteurella multocida (P. multocida) serotype B is associated with hemorrhagic septicaemia (HS) disease endemic in Africa, India and Asian countries. It is causative agent of thriftily significant disease in livestock. Hence, this study purposed and aimed to identify and express an immunogenic soluble protein of P. multocida in efforts toward development of HS vaccine. Immunogenic soluble protein was identified as lipoprotein B (plpB) using electrospray mass spectrometry. The size of expressed purified recombinant protein was approximately 39kDa. Immunogenicity study of the recombinant protein plpB was carried out using 6 groups o f BALB/c mice. The groups were immunized with recombinant protein (Group 1), soluble recombinant protein (Group 2), insoluble recombinant protein (Group 3), vector (Group 4), soluble protein o f P. multocida (Group 5) and PBS (Group 6 ) respectively. Mice in group 4 and 6 showed signs and symptom of HS after challenge with the parental strain (p-value < 0.05). However, immunised mice with purified recombinant protein did not show signs and symptoms of HS. Based on immunoblotting analysis, purified recombinant protein was significantly immunogenic (p-value < 0.05). Additionally, no inflammation was seen in the tissues o f organs from mice immunized with purified recombinant protein, which indicates that recombinant protein was 100% protective towards P. multocida infection and eventually towards HS disease. Thus, this study shows that the recombinant protein lipoprotein B (plpB) is significantly immunogenic and could be a potential candidate in developing vaccine against HS. 2015-04 Thesis https://ir.uitm.edu.my/id/eprint/27214/ https://ir.uitm.edu.my/id/eprint/27214/1/TM_NOOR%20MASYITAH%20JUMAHAT%20MD%2015_5.pdf text en public masters Universiti Teknologi MARA Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	RM Therapeutics Pharmacology RM Therapeutics Pharmacology
spellingShingle	RM Therapeutics Pharmacology RM Therapeutics Pharmacology Jumahat, Noor Masyitah Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
description	Pasteurella multocida (P. multocida) serotype B is associated with hemorrhagic septicaemia (HS) disease endemic in Africa, India and Asian countries. It is causative agent of thriftily significant disease in livestock. Hence, this study purposed and aimed to identify and express an immunogenic soluble protein of P. multocida in efforts toward development of HS vaccine. Immunogenic soluble protein was identified as lipoprotein B (plpB) using electrospray mass spectrometry. The size of expressed purified recombinant protein was approximately 39kDa. Immunogenicity study of the recombinant protein plpB was carried out using 6 groups o f BALB/c mice. The groups were immunized with recombinant protein (Group 1), soluble recombinant protein (Group 2), insoluble recombinant protein (Group 3), vector (Group 4), soluble protein o f P. multocida (Group 5) and PBS (Group 6 ) respectively. Mice in group 4 and 6 showed signs and symptom of HS after challenge with the parental strain (p-value < 0.05). However, immunised mice with purified recombinant protein did not show signs and symptoms of HS. Based on immunoblotting analysis, purified recombinant protein was significantly immunogenic (p-value < 0.05). Additionally, no inflammation was seen in the tissues o f organs from mice immunized with purified recombinant protein, which indicates that recombinant protein was 100% protective towards P. multocida infection and eventually towards HS disease. Thus, this study shows that the recombinant protein lipoprotein B (plpB) is significantly immunogenic and could be a potential candidate in developing vaccine against HS.
format	Thesis
qualification_level	Master's degree
author	Jumahat, Noor Masyitah
author_facet	Jumahat, Noor Masyitah
author_sort	Jumahat, Noor Masyitah
title	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_short	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_full	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_fullStr	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_full_unstemmed	Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat
title_sort	identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype b / noor masyitah jumahat
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Medicine
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/27214/1/TM_NOOR%20MASYITAH%20JUMAHAT%20MD%2015_5.pdf
_version_	1783733938981699584

Identification and immunogenicity study of soluble protein derived from pasteurella multocida serotype B / Noor Masyitah Jumahat

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