Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani

The biodegradability of petroleum waste product was evaluated using oil sludge taken from Exxon Mobil petroleum refiner y treatment plant in Kerteh, Terengganu. This study was a further extension of studies conducted by Dzulkafli (2007) where the qualitative analysis was established. The analysis in...

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Main Author:	Abdul Ghani, Norhafiza
Format:	Thesis
Language:	English
Published:	2008
Subjects:	Biodegradation
Online Access:	https://ir.uitm.edu.my/id/eprint/27241/1/TM_NORHAFIZA%20ABDUL%20GHANI%20EC%2008_5.pdf
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id	my-uitm-ir.27241
record_format	uketd_dc
spelling	my-uitm-ir.272412020-01-14T01:46:26Z Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani 2008 Abdul Ghani, Norhafiza Biodegradation TD Environmental technology. Sanitary engineering The biodegradability of petroleum waste product was evaluated using oil sludge taken from Exxon Mobil petroleum refiner y treatment plant in Kerteh, Terengganu. This study was a further extension of studies conducted by Dzulkafli (2007) where the qualitative analysis was established. The analysis involves the utilization of bacterial strains most effective in PAHs degradation as classified by Dzulkafli (2007). The PAHs adopted were phenanthrene, anthracene and dibenzothiophene which were used as source of carbon. PCR and DNA Sequencing were conducted to confirm the identity of bacterial strain classified by Dzulkafli (2007). These molecular technique s provide s up to 90% accuracy of identification. Using these techniques these three strains identified belong to genus of Pseudomonas namely; Uncultured Pseudomonas sp. clone 2-A, Pseudomonas Stutzeri strain HS-D36 and Pseudomonas Stutzeri strain LS40 1. Turbidity test was conducted for growth rates of each strain. Pseudomonas Stutzeri strain HS-D36 indicated the highest growth rates followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401. Identification of the most effective PAH degrading by bacterial strain was established by determining of average degradation rates. Anthracene proved to be the most degraded by these strains with the highest average degradation rate followed by phenanthrene and dibenzothiophene. While, Pseudomonas Stutzeri strain HS-D36 revealed to be the best strain in degrading PAHs, with the highest degradation followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401. 2008 Thesis https://ir.uitm.edu.my/id/eprint/27241/ https://ir.uitm.edu.my/id/eprint/27241/1/TM_NORHAFIZA%20ABDUL%20GHANI%20EC%2008_5.pdf text en public masters Universiti Teknologi MARA Faculty of Civil Engineering
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Biodegradation Biodegradation
spellingShingle	Biodegradation Biodegradation Abdul Ghani, Norhafiza Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
description	The biodegradability of petroleum waste product was evaluated using oil sludge taken from Exxon Mobil petroleum refiner y treatment plant in Kerteh, Terengganu. This study was a further extension of studies conducted by Dzulkafli (2007) where the qualitative analysis was established. The analysis involves the utilization of bacterial strains most effective in PAHs degradation as classified by Dzulkafli (2007). The PAHs adopted were phenanthrene, anthracene and dibenzothiophene which were used as source of carbon. PCR and DNA Sequencing were conducted to confirm the identity of bacterial strain classified by Dzulkafli (2007). These molecular technique s provide s up to 90% accuracy of identification. Using these techniques these three strains identified belong to genus of Pseudomonas namely; Uncultured Pseudomonas sp. clone 2-A, Pseudomonas Stutzeri strain HS-D36 and Pseudomonas Stutzeri strain LS40 1. Turbidity test was conducted for growth rates of each strain. Pseudomonas Stutzeri strain HS-D36 indicated the highest growth rates followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401. Identification of the most effective PAH degrading by bacterial strain was established by determining of average degradation rates. Anthracene proved to be the most degraded by these strains with the highest average degradation rate followed by phenanthrene and dibenzothiophene. While, Pseudomonas Stutzeri strain HS-D36 revealed to be the best strain in degrading PAHs, with the highest degradation followed by Uncultured Pseudomonas sp. clone 2-A and Pseudomonas Stutzeri strain LS401.
format	Thesis
qualification_level	Master's degree
author	Abdul Ghani, Norhafiza
author_facet	Abdul Ghani, Norhafiza
author_sort	Abdul Ghani, Norhafiza
title	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_short	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_full	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_fullStr	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_full_unstemmed	Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani
title_sort	quantitative determination on biodegradation of pahs by bacteria from oil sludge / norhafiza abdul ghani
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Civil Engineering
publishDate	2008
url	https://ir.uitm.edu.my/id/eprint/27241/1/TM_NORHAFIZA%20ABDUL%20GHANI%20EC%2008_5.pdf
_version_	1783733941721628672

Quantitative determination on biodegradation of PAHs by bacteria from oil sludge / Norhafiza Abdul Ghani

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