Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan

A total of 106 local white-rot fungi were screened for their ability to produce the enzyme laccase and to decolourise four textile dyes namely Remazol Brilliant Blue (RBB), Remazol Red (RR), Remazol Yellow (RY) and Procion Yellow (PY). Of these, 22 white-rot fungal isolates were able to produce lacc...

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Main Author:	Md. Kassim @ Hasan, Azlina
Format:	Thesis
Language:	English
Published:	2007
Subjects:	Dyes Textile industries
Online Access:	https://ir.uitm.edu.my/id/eprint/27268/2/27268.pdf
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id	my-uitm-ir.27268
record_format	uketd_dc
spelling	my-uitm-ir.272682023-11-16T01:01:46Z Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan 2007 Md. Kassim @ Hasan, Azlina Dyes Textile industries A total of 106 local white-rot fungi were screened for their ability to produce the enzyme laccase and to decolourise four textile dyes namely Remazol Brilliant Blue (RBB), Remazol Red (RR), Remazol Yellow (RY) and Procion Yellow (PY). Of these, 22 white-rot fungal isolates were able to produce laccase with activities exceeding 10 U/L. Among these 22 strains, seven decolourised RBB efficiently when cultured on solid media. Isolate UiTM80 was the most efficient decolourising strain and was able to decolourise RBB completely within 6 days. The corresponding laccase production in submerged fermentation reached a maximum of 67 U/L in 5 days. A Plackett-Burman Design and Response Surface Methodology (RSM) were employed to optimise the media for laccase production by UiTM80. Eight parameters were screened for their effects on laccase production; out of which pH and yeast extract were identified as the most significant media components. The RSM study suggested that the optimum media composition for laccase production is as follow: glucose, 10.0 giL; Kl-hP04, 0.50 g/L; trace elements, 5.00 mL/L; yeast extract, 9.83 g/L; ammonium tartarate, 5.24 g/L and pH 7.42. The experimental yield of laccase in this medium was 1,672 ± 950 U/L against a predicted yield of 1,850 U/L. Scale-up studies in a 5 litres-bioreactor showed that 10% inoculum (v/v) of UiTM80 was able to remove 91% of COD and 97% of colour from a 75 ppm RBB dye solution. In industrial textile wastewater, the fungus was able to remove 93% of COD and 33% of colour. 2007 Thesis https://ir.uitm.edu.my/id/eprint/27268/ https://ir.uitm.edu.my/id/eprint/27268/2/27268.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Dyes Textile industries
spellingShingle	Dyes Textile industries Md. Kassim @ Hasan, Azlina Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan
description	A total of 106 local white-rot fungi were screened for their ability to produce the enzyme laccase and to decolourise four textile dyes namely Remazol Brilliant Blue (RBB), Remazol Red (RR), Remazol Yellow (RY) and Procion Yellow (PY). Of these, 22 white-rot fungal isolates were able to produce laccase with activities exceeding 10 U/L. Among these 22 strains, seven decolourised RBB efficiently when cultured on solid media. Isolate UiTM80 was the most efficient decolourising strain and was able to decolourise RBB completely within 6 days. The corresponding laccase production in submerged fermentation reached a maximum of 67 U/L in 5 days. A Plackett-Burman Design and Response Surface Methodology (RSM) were employed to optimise the media for laccase production by UiTM80. Eight parameters were screened for their effects on laccase production; out of which pH and yeast extract were identified as the most significant media components. The RSM study suggested that the optimum media composition for laccase production is as follow: glucose, 10.0 giL; Kl-hP04, 0.50 g/L; trace elements, 5.00 mL/L; yeast extract, 9.83 g/L; ammonium tartarate, 5.24 g/L and pH 7.42. The experimental yield of laccase in this medium was 1,672 ± 950 U/L against a predicted yield of 1,850 U/L. Scale-up studies in a 5 litres-bioreactor showed that 10% inoculum (v/v) of UiTM80 was able to remove 91% of COD and 97% of colour from a 75 ppm RBB dye solution. In industrial textile wastewater, the fungus was able to remove 93% of COD and 33% of colour.
format	Thesis
qualification_level	Master's degree
author	Md. Kassim @ Hasan, Azlina
author_facet	Md. Kassim @ Hasan, Azlina
author_sort	Md. Kassim @ Hasan, Azlina
title	Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan
title_short	Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan
title_full	Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan
title_fullStr	Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan
title_full_unstemmed	Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan
title_sort	production of laccase and decolourisation of textile dyes by local white-rot fungi / azlina hj md. kassim @ hasan
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2007
url	https://ir.uitm.edu.my/id/eprint/27268/2/27268.pdf
_version_	1783733945630720000

Production of laccase and decolourisation of textile dyes by local white-rot fungi / Azlina Hj Md. Kassim @ Hasan

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