A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle

This study was conducted to verify the prevalence of cryptosporidiosis among children in Selangor. Consenting children aged 12 years and below, admitted with diarrhoea to Hospital Selayang and Hospital Sungai Buloh or contracting diarrhoea in hospitalization, were included as subjects. Each stool sa...

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Main Author:	Rossle, Nurul Fariza
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Infectious and parasitic diseases
Online Access:	https://ir.uitm.edu.my/id/eprint/27287/1/TM_NURUL%20FARIZA%20ROSSLE%20MD%2016_5.pdf
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id	my-uitm-ir.27287
record_format	uketd_dc
spelling	my-uitm-ir.272872022-06-21T07:19:40Z A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle 2016 Rossle, Nurul Fariza Infectious and parasitic diseases This study was conducted to verify the prevalence of cryptosporidiosis among children in Selangor. Consenting children aged 12 years and below, admitted with diarrhoea to Hospital Selayang and Hospital Sungai Buloh or contracting diarrhoea in hospitalization, were included as subjects. Each stool sample was distributed into six aliquots and tested for cryptosporidiosis using direct wet mount. Sheather’s sugar flotation, formalin-ether sedimentation, modified Ziehl-Neelsen staining, direct monoclonal fluorescent antibody, and polymerase chain reaction (PCR). Out of 160 stool samples, 6 (3.75%) were positive for Cryptosporidium spp. All children positive for cryptosporidiosis were aged <4 years. Direct wet mount, formalin-ether sedimentation concentration, modified Ziehl-Neelsen staining, and direct monoclonal fluorescent antibody testing showed the same number of positive samples (3.75%). Sheather's sugar flotation detected one less positive sample (3.13%). No noticeable DNA bands were observed on the electrophoresed gel from stool sample amplification, although there were faint bands for positive control using purified Cryptosporidium oocysts. The youngest child infected was 6 months old while the oldest was 3 years 4 months old. Same number of males and females were infected with four Malays and one Chinese and Indian each. There is no significant association between Cryptosporidium infection with gender and race. Age posed the most significant difference with all infected children being less than four years old. Further molecular epidemiological study on a larger scale incorporating humans, animals and environmental samples from the whole of Malaysia is recommended to clarify the dynamic of Cryptosporidium transmission in the country. 2016 Thesis https://ir.uitm.edu.my/id/eprint/27287/ https://ir.uitm.edu.my/id/eprint/27287/1/TM_NURUL%20FARIZA%20ROSSLE%20MD%2016_5.pdf text en public masters Universiti Teknologi MARA Faculty of Medicine
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Infectious and parasitic diseases
spellingShingle	Infectious and parasitic diseases Rossle, Nurul Fariza A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle
description	This study was conducted to verify the prevalence of cryptosporidiosis among children in Selangor. Consenting children aged 12 years and below, admitted with diarrhoea to Hospital Selayang and Hospital Sungai Buloh or contracting diarrhoea in hospitalization, were included as subjects. Each stool sample was distributed into six aliquots and tested for cryptosporidiosis using direct wet mount. Sheather’s sugar flotation, formalin-ether sedimentation, modified Ziehl-Neelsen staining, direct monoclonal fluorescent antibody, and polymerase chain reaction (PCR). Out of 160 stool samples, 6 (3.75%) were positive for Cryptosporidium spp. All children positive for cryptosporidiosis were aged <4 years. Direct wet mount, formalin-ether sedimentation concentration, modified Ziehl-Neelsen staining, and direct monoclonal fluorescent antibody testing showed the same number of positive samples (3.75%). Sheather's sugar flotation detected one less positive sample (3.13%). No noticeable DNA bands were observed on the electrophoresed gel from stool sample amplification, although there were faint bands for positive control using purified Cryptosporidium oocysts. The youngest child infected was 6 months old while the oldest was 3 years 4 months old. Same number of males and females were infected with four Malays and one Chinese and Indian each. There is no significant association between Cryptosporidium infection with gender and race. Age posed the most significant difference with all infected children being less than four years old. Further molecular epidemiological study on a larger scale incorporating humans, animals and environmental samples from the whole of Malaysia is recommended to clarify the dynamic of Cryptosporidium transmission in the country.
format	Thesis
qualification_level	Master's degree
author	Rossle, Nurul Fariza
author_facet	Rossle, Nurul Fariza
author_sort	Rossle, Nurul Fariza
title	A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle
title_short	A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle
title_full	A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle
title_fullStr	A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle
title_full_unstemmed	A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle
title_sort	study on cryptosporidium spp. isolated from children with diarrhoea admitted to selayang hospital and sungai buloh hospital in selangor, malaysia / nurul fariza rossle
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Medicine
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/27287/1/TM_NURUL%20FARIZA%20ROSSLE%20MD%2016_5.pdf
_version_	1783733949828169728

A study on cryptosporidium spp. isolated from children with diarrhoea admitted to Selayang Hospital and Sungai Buloh Hospital in Selangor, Malaysia / Nurul Fariza Rossle

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