Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim

The aim of the study was to determine the anti-ulcer effect of Elaeis guineensis Jacq. (Oil palm) leaves ethanolic extract on ethanol-induced ulcer in rats. In the present study, the rats were pre-treated with E. guineensis leaves (100, 200 and 500 mg/kg b.wt.). The phytochemical contents of the lea...

Full description

Saved in:

Bibliographic Details
Main Author:	Abd Rahim, Nurusyifa’
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Antioxidants
Online Access:	https://ir.uitm.edu.my/id/eprint/27324/1/TM_NURUSYIFA%27%20ABD%20RAHIM%20AS%2016_5.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.27324
record_format	uketd_dc
spelling	my-uitm-ir.273242022-06-23T02:18:27Z Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim 2016 Abd Rahim, Nurusyifa’ Antioxidants The aim of the study was to determine the anti-ulcer effect of Elaeis guineensis Jacq. (Oil palm) leaves ethanolic extract on ethanol-induced ulcer in rats. In the present study, the rats were pre-treated with E. guineensis leaves (100, 200 and 500 mg/kg b.wt.). The phytochemical contents of the leaves were screened using Gas Chromatography Mass Spectrophotometer (GC-MS). Meanwhile, antioxidant activity was evaluated using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay with five different concentrations (0.01, 0.05, 0.1, 0.15 and 0.2 mg/mL). From the results, the rats pre-treated with 100 mg/kg b.wt. Significantly (p<0.05) showed gastroprotective effect where only a small area of hemorrhagic lesions appeared on the gastric mucosal layer. Based on the phytochemical screening by GC-MS, the presence of phytol was detected. Phytol is known as diterpenoids, which can be found in chlorophyll and tocopherol. They are known as strong immunomodulatory agent, which is known as one of the components that can increase human immune system in fighting antigens. Antioxidant assay exhibited good antioxidant activity from the ethanolic extract with IC50 value of 0.0817 mg/mL. IC50 value less than 10 mg/mL is considered as an effective antioxidant. The extract at high concentration (0.2 mg/mL) showed the highest percentage of DPPH inhibition by antioxidant with 67.06%. Thus, explaining the extract at high concentration has higher antioxidant activity compare to lower concentration. In the anti-ulcer study, an increase in concentration E. guineensis (0.2 mg/mL), led to an increase in ulcer area. This could be due to the fact that phytol are potentially toxic at high concentration, although they are also good immunomodulatory agents. The extract at low concentration showed an effective gastroprotective activity as compared to the extracts at higher concentrations. Thus, E. guineensis leaves appear to have therapeutic potential in preventing gastric ulceration induced by ethanol. 2016 Thesis https://ir.uitm.edu.my/id/eprint/27324/ https://ir.uitm.edu.my/id/eprint/27324/1/TM_NURUSYIFA%27%20ABD%20RAHIM%20AS%2016_5.pdf text en public masters Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Antioxidants
spellingShingle	Antioxidants Abd Rahim, Nurusyifa’ Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
description	The aim of the study was to determine the anti-ulcer effect of Elaeis guineensis Jacq. (Oil palm) leaves ethanolic extract on ethanol-induced ulcer in rats. In the present study, the rats were pre-treated with E. guineensis leaves (100, 200 and 500 mg/kg b.wt.). The phytochemical contents of the leaves were screened using Gas Chromatography Mass Spectrophotometer (GC-MS). Meanwhile, antioxidant activity was evaluated using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay with five different concentrations (0.01, 0.05, 0.1, 0.15 and 0.2 mg/mL). From the results, the rats pre-treated with 100 mg/kg b.wt. Significantly (p<0.05) showed gastroprotective effect where only a small area of hemorrhagic lesions appeared on the gastric mucosal layer. Based on the phytochemical screening by GC-MS, the presence of phytol was detected. Phytol is known as diterpenoids, which can be found in chlorophyll and tocopherol. They are known as strong immunomodulatory agent, which is known as one of the components that can increase human immune system in fighting antigens. Antioxidant assay exhibited good antioxidant activity from the ethanolic extract with IC50 value of 0.0817 mg/mL. IC50 value less than 10 mg/mL is considered as an effective antioxidant. The extract at high concentration (0.2 mg/mL) showed the highest percentage of DPPH inhibition by antioxidant with 67.06%. Thus, explaining the extract at high concentration has higher antioxidant activity compare to lower concentration. In the anti-ulcer study, an increase in concentration E. guineensis (0.2 mg/mL), led to an increase in ulcer area. This could be due to the fact that phytol are potentially toxic at high concentration, although they are also good immunomodulatory agents. The extract at low concentration showed an effective gastroprotective activity as compared to the extracts at higher concentrations. Thus, E. guineensis leaves appear to have therapeutic potential in preventing gastric ulceration induced by ethanol.
format	Thesis
qualification_level	Master's degree
author	Abd Rahim, Nurusyifa’
author_facet	Abd Rahim, Nurusyifa’
author_sort	Abd Rahim, Nurusyifa’
title	Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_short	Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_full	Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_fullStr	Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_full_unstemmed	Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim
title_sort	antioxidant and anti-ulcer effect of elaeis guineensis jacq. (oil palm) leaves ethanolic extract on experimental rats / nurusyifa’ abd rahim
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/27324/1/TM_NURUSYIFA%27%20ABD%20RAHIM%20AS%2016_5.pdf
_version_	1783733953464631296

Antioxidant and anti-ulcer effect of Elaeis Guineensis Jacq. (Oil Palm) leaves ethanolic extract on experimental rats / Nurusyifa’ Abd Rahim

Similar Items