Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong

Aedes albopictus is a significant vector capable of transmitting dengue virus (DENV) and Chikungunya virus (CHIKV). Aedes albopictus has heat shock protein 70 (HSP70) protein molecules that are receptor complexes associated with virus replication. Thus, it have been implicated in viral entry, propag...

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Main Author:	Sulong, Nur Azimah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	QP Physiology Special situations and conditions
Online Access:	https://ir.uitm.edu.my/id/eprint/27369/1/TD_NUR%20AZIMAH%20SULONG%20HS%2015_5.pdf
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id	my-uitm-ir.27369
record_format	uketd_dc
spelling	my-uitm-ir.273692020-01-21T03:46:35Z Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong 2015 Sulong, Nur Azimah QP Physiology Special situations and conditions Aedes albopictus is a significant vector capable of transmitting dengue virus (DENV) and Chikungunya virus (CHIKV). Aedes albopictus has heat shock protein 70 (HSP70) protein molecules that are receptor complexes associated with virus replication. Thus, it have been implicated in viral entry, propagation, and transmission of DENV. This study was performed to determine the optimum media and laboratory conditions for the detection of HSP70 extracted from salivary gland tissues from field collected Aedes albopictus mosquitoes. Proteins were subjected to a variety of temperature and media, and its 70 kDa profile, analysed by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Based on the protein profile analysis, protein band of 70 kDa was distinctly detected when preserved in phosphate buffered saline (PBS) and stored at 4°C for a period of 7 days. This protein is analogous to the protein synthesized by dengue infected Aedes albopictus. It is required for the entry of DENV in human cells such as in human monocytes and macrophages. Thus, further study utilising amino acid sequencing must be performed to confirm the presence of HSP70 which has been revealed by SDS-PAGE protein analysis. 2015 Thesis https://ir.uitm.edu.my/id/eprint/27369/ https://ir.uitm.edu.my/id/eprint/27369/1/TD_NUR%20AZIMAH%20SULONG%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	QP Physiology Special situations and conditions
spellingShingle	QP Physiology Special situations and conditions Sulong, Nur Azimah Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong
description	Aedes albopictus is a significant vector capable of transmitting dengue virus (DENV) and Chikungunya virus (CHIKV). Aedes albopictus has heat shock protein 70 (HSP70) protein molecules that are receptor complexes associated with virus replication. Thus, it have been implicated in viral entry, propagation, and transmission of DENV. This study was performed to determine the optimum media and laboratory conditions for the detection of HSP70 extracted from salivary gland tissues from field collected Aedes albopictus mosquitoes. Proteins were subjected to a variety of temperature and media, and its 70 kDa profile, analysed by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Based on the protein profile analysis, protein band of 70 kDa was distinctly detected when preserved in phosphate buffered saline (PBS) and stored at 4°C for a period of 7 days. This protein is analogous to the protein synthesized by dengue infected Aedes albopictus. It is required for the entry of DENV in human cells such as in human monocytes and macrophages. Thus, further study utilising amino acid sequencing must be performed to confirm the presence of HSP70 which has been revealed by SDS-PAGE protein analysis.
format	Thesis
qualification_level	Bachelor degree
author	Sulong, Nur Azimah
author_facet	Sulong, Nur Azimah
author_sort	Sulong, Nur Azimah
title	Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong
title_short	Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong
title_full	Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong
title_fullStr	Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong
title_full_unstemmed	Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong
title_sort	optimization of storage conditions for the detection of heat shock protein 70 (hsp70) from salivary glands of field collected aedes albopictus / nur azimah sulong
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/27369/1/TD_NUR%20AZIMAH%20SULONG%20HS%2015_5.pdf
_version_	1783733958596362240

Optimization of storage conditions for the detection of Heat Shock Protein 70 (HSP70) from salivary glands of field collected Aedes albopictus / Nur Azimah Sulong

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