Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin

Marine-derived actinomycetes possess distinct and complex metabolic capabilities, resulting in the wide diversity of their secondary metabolites in terms of chemical structure and biological activity. The bioactive compounds of actinomycetes isolated from mangrove soils are reported to possess vario...

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Main Author:	Ariffin, Suhaidi
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Plant physiology
Online Access:	https://ir.uitm.edu.my/id/eprint/27582/1/TP_SUHAIDI%20ARIFFIN%20AS%2015_5.pdf
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id	my-uitm-ir.27582
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spelling	my-uitm-ir.275822022-06-13T07:40:31Z Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin 2015 Ariffin, Suhaidi Plant physiology Marine-derived actinomycetes possess distinct and complex metabolic capabilities, resulting in the wide diversity of their secondary metabolites in terms of chemical structure and biological activity. The bioactive compounds of actinomycetes isolated from mangrove soils are reported to possess various biological activities such as antimicrobial, cytotoxicity, anticancer and antioxidant. The aims of this study are to isolate and characterize diverse populations of marine actinomycetes from mangrove soils, to identify selected morphologically distinct isolates by using 16S rRNA sequence analysis, to screen their potential biological activities as antimicrobial as well as β-lactamase inhibitor agents, and to elucidate the structure of selected bioactive compounds. A total of 73 actinomycete strains were isolated from II mangrove locations in Malaysia. Of these, morphological observations and 16S rRNA sequence analysis indicate the presence of representative species from at least 3 genera Streptomyces, Pseudonocardia and Saccharomonospora. The majority of the isolates belong to genus Streptomyces. It was found that the most productive medium for isolation is starch casein nitrate agar (SCNA). It was also found that addition of 3% sodium chloride (NaCl) improved the isolation rate of mangrove actinomycetes. Disc diffusion assay showed that 9.6% (direct broth culture) and 8.2% (ethyl acetate extract) of these isolates were able to produce antimicrobial compounds active against Staphylococcus aureus, Bacillus subtilis, Candida albicans as well as Saccharomyces cerevisiae. Hexane extracts however exhibited no activity. A modified microdilution plate assay showed that 16.4% (ethyl acetate extract) of these strains were able to generate antimicrobial compounds active against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Candida albicans. A rapid screening assay for β-lactamase inhibitors activity by resazurin microdilution plate assay was developed and showed that 4.9% of actinomycete isolates were able to produce inhibitor compounds against Staphylococcus aureus ATCC 43300. The KMS I isolate was chosen as a lead candidate because this strain showed consistent production of antimicrobial and inhibitor active compounds throughout the screening experiments. Nuclear magnetic resonance (NMR) spectroscopy analysis showed that the active compound (KMS 12B) was 4-hydroxybenzoic acid. 2015 Thesis https://ir.uitm.edu.my/id/eprint/27582/ https://ir.uitm.edu.my/id/eprint/27582/1/TP_SUHAIDI%20ARIFFIN%20AS%2015_5.pdf text en public phd doctoral Universiti Teknologi MARA Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Plant physiology
spellingShingle	Plant physiology Ariffin, Suhaidi Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin
description	Marine-derived actinomycetes possess distinct and complex metabolic capabilities, resulting in the wide diversity of their secondary metabolites in terms of chemical structure and biological activity. The bioactive compounds of actinomycetes isolated from mangrove soils are reported to possess various biological activities such as antimicrobial, cytotoxicity, anticancer and antioxidant. The aims of this study are to isolate and characterize diverse populations of marine actinomycetes from mangrove soils, to identify selected morphologically distinct isolates by using 16S rRNA sequence analysis, to screen their potential biological activities as antimicrobial as well as β-lactamase inhibitor agents, and to elucidate the structure of selected bioactive compounds. A total of 73 actinomycete strains were isolated from II mangrove locations in Malaysia. Of these, morphological observations and 16S rRNA sequence analysis indicate the presence of representative species from at least 3 genera Streptomyces, Pseudonocardia and Saccharomonospora. The majority of the isolates belong to genus Streptomyces. It was found that the most productive medium for isolation is starch casein nitrate agar (SCNA). It was also found that addition of 3% sodium chloride (NaCl) improved the isolation rate of mangrove actinomycetes. Disc diffusion assay showed that 9.6% (direct broth culture) and 8.2% (ethyl acetate extract) of these isolates were able to produce antimicrobial compounds active against Staphylococcus aureus, Bacillus subtilis, Candida albicans as well as Saccharomyces cerevisiae. Hexane extracts however exhibited no activity. A modified microdilution plate assay showed that 16.4% (ethyl acetate extract) of these strains were able to generate antimicrobial compounds active against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Candida albicans. A rapid screening assay for β-lactamase inhibitors activity by resazurin microdilution plate assay was developed and showed that 4.9% of actinomycete isolates were able to produce inhibitor compounds against Staphylococcus aureus ATCC 43300. The KMS I isolate was chosen as a lead candidate because this strain showed consistent production of antimicrobial and inhibitor active compounds throughout the screening experiments. Nuclear magnetic resonance (NMR) spectroscopy analysis showed that the active compound (KMS 12B) was 4-hydroxybenzoic acid.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Ariffin, Suhaidi
author_facet	Ariffin, Suhaidi
author_sort	Ariffin, Suhaidi
title	Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin
title_short	Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin
title_full	Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin
title_fullStr	Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin
title_full_unstemmed	Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin
title_sort	screening for β-lactamase inhibitors from mangrove soil actinomycetes / suhaidi ariffin
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/27582/1/TP_SUHAIDI%20ARIFFIN%20AS%2015_5.pdf
_version_	1783733977105825792

Screening for β-lactamase inhibitors from mangrove soil actinomycetes / Suhaidi Ariffin

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