Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari

Enterocytozaon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidjosis is also higher in immunooompetent individuals. The previous st...

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Main Author: Azhari, Azah Ashikin
Format: Thesis
Language:English
Published: 2016
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/27694/1/TD_AZAH%20ASHIKIN%20AZHARI%20HS%2015_5.pdf
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spelling my-uitm-ir.276942020-02-05T07:37:40Z Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari 2016 Azhari, Azah Ashikin Immunology Bacteria Clinical pathology. Laboratory technique Enterocytozaon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidjosis is also higher in immunooompetent individuals. The previous study of microsporidial infection is mostly focused on immunodeficiency or immunosuppressive individuals. Information regarding microsporidial infections among immunocompetent individuals is lacking. Hence, this cross sectional study was carried out to determine prevalence of E‘ bieneusi infection and to find out the occurrence of E. bieneusi according to age group and gender among the Prom-Malay and Senoi tribes in Sungai Lembing, Pahang, Malaysia. Stool samples were collected fiom 209 voluntary participants; 96 males and 113 females that aged between one to 85 years old. Demographic data, socioeconomic, environmental, and behavioral infomation was collected by using pre-tested questionnaire. Overall prevalence of E‘ bieneusi infection was 3.83 % (8/209). From the observation, majority of individual infected with E. bieneusi was male (833 %) can be observed in those aged 215 years old‘ These samples were detected Small Subunit rDNA gene by using Polymerase chain reaction amplification with specific primer EBIEFl and EBIER], as demonstrated by an amplicon of 607-bp. This is the first report of E‘ bieneusi in Malaysia. It is hoped that these findings will allow greater emphasis from public health authrities through public education on personal hygiene and the consumption of boiled or filtered water in order to reduce the infection. 2016 Thesis https://ir.uitm.edu.my/id/eprint/27694/ https://ir.uitm.edu.my/id/eprint/27694/1/TD_AZAH%20ASHIKIN%20AZHARI%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Science
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
topic Immunology
Bacteria
Immunology
spellingShingle Immunology
Bacteria
Immunology
Azhari, Azah Ashikin
Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
description Enterocytozaon bieneusi is most common of microsporidian species that infects many types of cells including human and animals. These organisms are causing long lasting diarrhea primarily in immunocompromised patients but microsporidjosis is also higher in immunooompetent individuals. The previous study of microsporidial infection is mostly focused on immunodeficiency or immunosuppressive individuals. Information regarding microsporidial infections among immunocompetent individuals is lacking. Hence, this cross sectional study was carried out to determine prevalence of E‘ bieneusi infection and to find out the occurrence of E. bieneusi according to age group and gender among the Prom-Malay and Senoi tribes in Sungai Lembing, Pahang, Malaysia. Stool samples were collected fiom 209 voluntary participants; 96 males and 113 females that aged between one to 85 years old. Demographic data, socioeconomic, environmental, and behavioral infomation was collected by using pre-tested questionnaire. Overall prevalence of E‘ bieneusi infection was 3.83 % (8/209). From the observation, majority of individual infected with E. bieneusi was male (833 %) can be observed in those aged 215 years old‘ These samples were detected Small Subunit rDNA gene by using Polymerase chain reaction amplification with specific primer EBIEFl and EBIER], as demonstrated by an amplicon of 607-bp. This is the first report of E‘ bieneusi in Malaysia. It is hoped that these findings will allow greater emphasis from public health authrities through public education on personal hygiene and the consumption of boiled or filtered water in order to reduce the infection.
format Thesis
qualification_level Bachelor degree
author Azhari, Azah Ashikin
author_facet Azhari, Azah Ashikin
author_sort Azhari, Azah Ashikin
title Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_short Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_full Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_fullStr Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_full_unstemmed Identification and characterization of entermytazuun bieneusi isolates from the Proto—Malay and Senoi tribes in Sungai Lembing, Pahang / Azah Ashikin Azhari
title_sort identification and characterization of entermytazuun bieneusi isolates from the proto—malay and senoi tribes in sungai lembing, pahang / azah ashikin azhari
granting_institution Universiti Teknologi MARA
granting_department Faculty of Health Science
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/27694/1/TD_AZAH%20ASHIKIN%20AZHARI%20HS%2015_5.pdf
_version_ 1783733994970415104