Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah

Ionizing radiation is one of a radicals sources inside human body which resulted in oxidative damage. Overproduction of ROS and imbalance of antioxidant production can contribute to various pathological condition such as cancer and tissues injuries. To date, lack of information about capability of w...

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Main Author:	Hanifah, Ruzaana
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Clinical pathology Laboratory technique
Online Access:	https://ir.uitm.edu.my/id/eprint/27801/1/TD_RUZAANA%20HANIFAH%20HS%2015_5.pdf
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id	my-uitm-ir.27801
record_format	uketd_dc
spelling	my-uitm-ir.278012020-02-22T16:01:03Z Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah 2015 Hanifah, Ruzaana Clinical pathology. Laboratory technique Ionizing radiation is one of a radicals sources inside human body which resulted in oxidative damage. Overproduction of ROS and imbalance of antioxidant production can contribute to various pathological condition such as cancer and tissues injuries. To date, lack of information about capability of watermelon in reducing oxidative damage cause by oxidant such as IR. Therefore, the present study was aimed to evaluate the radio protective effect of 50% watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice against low dose ionizing irradiation - induced oxidative stress in liver. Eighteen healthy male ICR mice was randomly divided into three group consist of negative control group, radiation and treatment groups (n=6). Negative control and radiation groups mice received filtered tap water, but radiation group was exposed to low dose radiation (IOOflGy) on day 15 whereas treatment group was supplemented with 50 % of watermelon juice ad libitum for 14 days prior to total body X-ray exposure in single dose. SODs inhibition activities, total glutathione (GSH) and Malondialdehye (MDA) level was assessed in mice liver. Level of DNA damage was studied by measuring olive tail moment using Comet assay. The SOD inhibition activities and GSH level showed significant reduction in radiation and treatment group compared to negative control group (P < 0.05), however no significant marked between both groups although treatment group had higher mean compared to radiation group. MDA level among all the groups showed no significant differences, but in treatment group showed decrease of MDA level compared to radiation group. However, finding of DNA damage observed significantly increased in radiation group compared to negative control and treatment groups (P < 0.05). In conclusion, present study reveal biological evidence that support radio protection effect of 50% watermelon juice in molecular changes and several biochemical changes against radiation-induced oxidative damage for 14 days in mice liver. 2015 Thesis https://ir.uitm.edu.my/id/eprint/27801/ https://ir.uitm.edu.my/id/eprint/27801/1/TD_RUZAANA%20HANIFAH%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Clinical pathology Laboratory technique
spellingShingle	Clinical pathology Laboratory technique Hanifah, Ruzaana Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah
description	Ionizing radiation is one of a radicals sources inside human body which resulted in oxidative damage. Overproduction of ROS and imbalance of antioxidant production can contribute to various pathological condition such as cancer and tissues injuries. To date, lack of information about capability of watermelon in reducing oxidative damage cause by oxidant such as IR. Therefore, the present study was aimed to evaluate the radio protective effect of 50% watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice against low dose ionizing irradiation - induced oxidative stress in liver. Eighteen healthy male ICR mice was randomly divided into three group consist of negative control group, radiation and treatment groups (n=6). Negative control and radiation groups mice received filtered tap water, but radiation group was exposed to low dose radiation (IOOflGy) on day 15 whereas treatment group was supplemented with 50 % of watermelon juice ad libitum for 14 days prior to total body X-ray exposure in single dose. SODs inhibition activities, total glutathione (GSH) and Malondialdehye (MDA) level was assessed in mice liver. Level of DNA damage was studied by measuring olive tail moment using Comet assay. The SOD inhibition activities and GSH level showed significant reduction in radiation and treatment group compared to negative control group (P < 0.05), however no significant marked between both groups although treatment group had higher mean compared to radiation group. MDA level among all the groups showed no significant differences, but in treatment group showed decrease of MDA level compared to radiation group. However, finding of DNA damage observed significantly increased in radiation group compared to negative control and treatment groups (P < 0.05). In conclusion, present study reveal biological evidence that support radio protection effect of 50% watermelon juice in molecular changes and several biochemical changes against radiation-induced oxidative damage for 14 days in mice liver.
format	Thesis
qualification_level	Bachelor degree
author	Hanifah, Ruzaana
author_facet	Hanifah, Ruzaana
author_sort	Hanifah, Ruzaana
title	Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah
title_short	Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah
title_full	Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah
title_fullStr	Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah
title_full_unstemmed	Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah
title_sort	radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / ruzaana hanifah
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/27801/1/TD_RUZAANA%20HANIFAH%20HS%2015_5.pdf
_version_	1783734013216686080

Radio protective effect of 500/0 watermelon (citrullus lanatus (thunb.) matsum. and nakai) juice in liver / Ruzaana Hanifah

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