Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan

DEL is the weakest RhD-positive phenotype that commonly mistyped as RhDnegative. It may induce alloimmunization when transfused to RhD-negative recipients. Serologically, it can only be detected via adsorption-elution test. In Malaysia, few data exist regarding DEL phenotype. The objective of this s...

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Main Author:	Maswan, Nor Elizatul Izzati
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Examination of the blood
Online Access:	https://ir.uitm.edu.my/id/eprint/28031/1/TD_NOR%20ELIZATUL%20IZZATI%20MASWAN%20HS%2015_5.pdf
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id	my-uitm-ir.28031
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spelling	my-uitm-ir.280312020-02-11T03:40:13Z Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan 2015 Maswan, Nor Elizatul Izzati Examination of the blood DEL is the weakest RhD-positive phenotype that commonly mistyped as RhDnegative. It may induce alloimmunization when transfused to RhD-negative recipients. Serologically, it can only be detected via adsorption-elution test. In Malaysia, few data exist regarding DEL phenotype. The objective of this study was to detect DEL phenotype in RhD-negative blood using adsorption-elution technique and Sequence Specific Primer-Polymerase Chain Reaction (SSP-PCR). A total of 43 RhD-negative blood samples were collected from Pusat Darah Negara. Rh phenotype for each sample was tested. Heat elution by incubation at 56°C for 10 minutes were implemented. Indirect Antiglobulin Test against Rh(+) cells and Rh(-) cells were completed on the eluates and last wash supernatant. Then, identification of DEL carrying RHD1227A was performed via SSP-PCR. Rh-phenotype identified were ccee with 79.07%, Ccee with 13.95%,4.65% of ccEe phenotype and CCee phenotype with only 2.33%. One (2.33%) out of 43 samples was identified as DEL phenotype carrying RHDI227A allele when tested using SSP-PCR but none was identified from adsorption-elution. SSP-PCR was more sensitive and specific compared to adsorption-elution test. Hence, implementation of SSP-PCR for efficient DEL phenotypes typing is highly recommended. 2015 Thesis https://ir.uitm.edu.my/id/eprint/28031/ https://ir.uitm.edu.my/id/eprint/28031/1/TD_NOR%20ELIZATUL%20IZZATI%20MASWAN%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Examination of the blood
spellingShingle	Examination of the blood Maswan, Nor Elizatul Izzati Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan
description	DEL is the weakest RhD-positive phenotype that commonly mistyped as RhDnegative. It may induce alloimmunization when transfused to RhD-negative recipients. Serologically, it can only be detected via adsorption-elution test. In Malaysia, few data exist regarding DEL phenotype. The objective of this study was to detect DEL phenotype in RhD-negative blood using adsorption-elution technique and Sequence Specific Primer-Polymerase Chain Reaction (SSP-PCR). A total of 43 RhD-negative blood samples were collected from Pusat Darah Negara. Rh phenotype for each sample was tested. Heat elution by incubation at 56°C for 10 minutes were implemented. Indirect Antiglobulin Test against Rh(+) cells and Rh(-) cells were completed on the eluates and last wash supernatant. Then, identification of DEL carrying RHD1227A was performed via SSP-PCR. Rh-phenotype identified were ccee with 79.07%, Ccee with 13.95%,4.65% of ccEe phenotype and CCee phenotype with only 2.33%. One (2.33%) out of 43 samples was identified as DEL phenotype carrying RHDI227A allele when tested using SSP-PCR but none was identified from adsorption-elution. SSP-PCR was more sensitive and specific compared to adsorption-elution test. Hence, implementation of SSP-PCR for efficient DEL phenotypes typing is highly recommended.
format	Thesis
qualification_level	Bachelor degree
author	Maswan, Nor Elizatul Izzati
author_facet	Maswan, Nor Elizatul Izzati
author_sort	Maswan, Nor Elizatul Izzati
title	Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan
title_short	Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan
title_full	Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan
title_fullStr	Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan
title_full_unstemmed	Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan
title_sort	detection of del phenotype in rhd-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / nor elizatul izzati maswan
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/28031/1/TD_NOR%20ELIZATUL%20IZZATI%20MASWAN%20HS%2015_5.pdf
_version_	1783734038894215168

Detection of Del Phenotype in RHD-negative blood using heat elution technique and sequence specific primerpolymerase chain reaction / Nor Elizatul Izzati Maswan

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