In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli

Thromboembolic is a disorder which rose up from the abnormal haemostasis process. Most of anticoagulant drug used in the treatment and prevention of thromboembolic disorder caused side effects such as bleeding. Potential of Rhodomyrtus tomentosa as a native anticoagulant been tested by evaluating th...

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Main Author:	Adli, Norizzaty
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Clinical pathology Laboratory technique
Online Access:	https://ir.uitm.edu.my/id/eprint/28053/1/TD_NORIZZATY%20ADLI%20HS%2015_5.pdf
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id	my-uitm-ir.28053
record_format	uketd_dc
spelling	my-uitm-ir.280532020-02-03T04:40:59Z In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli 2015 Adli, Norizzaty Clinical pathology. Laboratory technique Thromboembolic is a disorder which rose up from the abnormal haemostasis process. Most of anticoagulant drug used in the treatment and prevention of thromboembolic disorder caused side effects such as bleeding. Potential of Rhodomyrtus tomentosa as a native anticoagulant been tested by evaluating the possible haemostatic effect of Rhodomyrtus tomentosa aqueous leaf extract in vitro. In vitro coagulation assays such as Thrombin time (TT), Prothrombin time (PT) and Activated partial thromboplastin time (APTT) tests were performed on normal coagulation control plasma spiked with different concentrations of the aqueous leaf extract (10 - 100%). Total tannin and flavonoid for all different concentrations of leaf extract were measured using spectrophotometer and correlate with clotting times (TT, PT and APTT). The results were analyzed using one way ANOVA followed by post-hoc Dunnet's and Pearson's correlation. The results showed that TT, PT and APTT of normal coagulation control plasma spiked with extract were markedly prolonged. A significant anticoagulant (p < 0.05) showed by PT was at lowest concentration (10%), TT was at concentration 50 - 100% and APTT was at concentration 80% 100%. The aqueous extraction showed a better anticoagulant for PT. The clotting times for TT, PT and APTT were significantly correlated (p < 0.05) with total tannin and flavonoid. Besides, the total tannin and flavonoid was significantly decreased (p < 0.05) when exposed to the light for 5 days. In conclusion, the results highlights that Rhodomyrtus tomentosa contain anticoagulant properties reflected the bioactive compound of tannin and flavonoid. 2015 Thesis https://ir.uitm.edu.my/id/eprint/28053/ https://ir.uitm.edu.my/id/eprint/28053/1/TD_NORIZZATY%20ADLI%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Clinical pathology Laboratory technique
spellingShingle	Clinical pathology Laboratory technique Adli, Norizzaty In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
description	Thromboembolic is a disorder which rose up from the abnormal haemostasis process. Most of anticoagulant drug used in the treatment and prevention of thromboembolic disorder caused side effects such as bleeding. Potential of Rhodomyrtus tomentosa as a native anticoagulant been tested by evaluating the possible haemostatic effect of Rhodomyrtus tomentosa aqueous leaf extract in vitro. In vitro coagulation assays such as Thrombin time (TT), Prothrombin time (PT) and Activated partial thromboplastin time (APTT) tests were performed on normal coagulation control plasma spiked with different concentrations of the aqueous leaf extract (10 - 100%). Total tannin and flavonoid for all different concentrations of leaf extract were measured using spectrophotometer and correlate with clotting times (TT, PT and APTT). The results were analyzed using one way ANOVA followed by post-hoc Dunnet's and Pearson's correlation. The results showed that TT, PT and APTT of normal coagulation control plasma spiked with extract were markedly prolonged. A significant anticoagulant (p < 0.05) showed by PT was at lowest concentration (10%), TT was at concentration 50 - 100% and APTT was at concentration 80% 100%. The aqueous extraction showed a better anticoagulant for PT. The clotting times for TT, PT and APTT were significantly correlated (p < 0.05) with total tannin and flavonoid. Besides, the total tannin and flavonoid was significantly decreased (p < 0.05) when exposed to the light for 5 days. In conclusion, the results highlights that Rhodomyrtus tomentosa contain anticoagulant properties reflected the bioactive compound of tannin and flavonoid.
format	Thesis
qualification_level	Bachelor degree
author	Adli, Norizzaty
author_facet	Adli, Norizzaty
author_sort	Adli, Norizzaty
title	In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_short	In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_full	In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_fullStr	In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_full_unstemmed	In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli
title_sort	in vitro haemostatic activity of rhodomyrtus tomentosa (aiton) hassk. aqueous leaf extract / norizzaty adli
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/28053/1/TD_NORIZZATY%20ADLI%20HS%2015_5.pdf
_version_	1783734044774629376

In vitro haemostatic activity of Rhodomyrtus Tomentosa (Aiton) Hassk. Aqueous leaf extract / Norizzaty Adli

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