Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan

Streptococcus pneumonia is the main cause of pneumococcal diseases ranging from non-invasive condition of otitis media and sinusitis to more invasive conditions of meningitis and septicemia particularly in children, elderly and immunocompromised. Virulence genes found in Streptococcus pneumoniae are...

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Main Author:	Nor Azlan, Yuhaniz Atiqah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Virology
Online Access:	https://ir.uitm.edu.my/id/eprint/28068/1/TD_YUHANIZ%20ATIQAH%20NOR%20AZLAN%20HS%2015_5.pdf
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spelling	my-uitm-ir.280682020-01-31T07:40:58Z Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan 2015 Nor Azlan, Yuhaniz Atiqah Virology Clinical pathology. Laboratory technique Streptococcus pneumonia is the main cause of pneumococcal diseases ranging from non-invasive condition of otitis media and sinusitis to more invasive conditions of meningitis and septicemia particularly in children, elderly and immunocompromised. Virulence genes found in Streptococcus pneumoniae are lytA, ply, PsaA, PspA, cbpA, PavA, nanA, nanB, eno, and psrP. There is limited information about the distribution of virulence genes in Streptococcus pneumoniae isolates from laboratory strains Streptococcus pneumoniae in comparison with clinical strains. Therefore, this study was conducted to detect virulence genes (lytA, ply, PsaA, PspA, cbpA, PavA, nanA, nanB, eno, and psrP) and to compare the virulence genes distribution in Streptococcus pneumoniae from laboratory reference strains with clinical strains. A sample Streptococcus pneumoniae (ATCC 6305) from laboratory strains was obtained from Microbiology laboratory, UiTM Puncak Alam and the bacterial DNA was extracted using the boiling method. The detection of lytA, ply, PsaA, PspA, cbp A, PavA, nanA, nanB, eno, and psrP in Streptococcus pneumoniae from laboratory reference strains was done using SYBR Green real-time PCR. In this study, the biochemical and molecular tests confirmed the isolates as Streptococcus pneumoniae (ATCC 6305). The real-time PCR showed that all isolates were positive for virulence genes LytA, Ply, NanB and PspA genes. No amplification was seen for PsaA, cbpA, PavA, nanA, eno and psrP genes. Hence, this study indicates that, with such genotypic distribution pattern, Streptococcus pneumoniae from laboratory strains is a less pathogenicity as only four virulence genes detected compared with virulence gene's presence in the clinical strains. The application of real-time PCR proved a rapid and specific method for the detection of virulence genes in Streptococcus pneumoniae. 2015 Thesis https://ir.uitm.edu.my/id/eprint/28068/ https://ir.uitm.edu.my/id/eprint/28068/1/TD_YUHANIZ%20ATIQAH%20NOR%20AZLAN%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Virology Virology
spellingShingle	Virology Virology Nor Azlan, Yuhaniz Atiqah Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan
description	Streptococcus pneumonia is the main cause of pneumococcal diseases ranging from non-invasive condition of otitis media and sinusitis to more invasive conditions of meningitis and septicemia particularly in children, elderly and immunocompromised. Virulence genes found in Streptococcus pneumoniae are lytA, ply, PsaA, PspA, cbpA, PavA, nanA, nanB, eno, and psrP. There is limited information about the distribution of virulence genes in Streptococcus pneumoniae isolates from laboratory strains Streptococcus pneumoniae in comparison with clinical strains. Therefore, this study was conducted to detect virulence genes (lytA, ply, PsaA, PspA, cbpA, PavA, nanA, nanB, eno, and psrP) and to compare the virulence genes distribution in Streptococcus pneumoniae from laboratory reference strains with clinical strains. A sample Streptococcus pneumoniae (ATCC 6305) from laboratory strains was obtained from Microbiology laboratory, UiTM Puncak Alam and the bacterial DNA was extracted using the boiling method. The detection of lytA, ply, PsaA, PspA, cbp A, PavA, nanA, nanB, eno, and psrP in Streptococcus pneumoniae from laboratory reference strains was done using SYBR Green real-time PCR. In this study, the biochemical and molecular tests confirmed the isolates as Streptococcus pneumoniae (ATCC 6305). The real-time PCR showed that all isolates were positive for virulence genes LytA, Ply, NanB and PspA genes. No amplification was seen for PsaA, cbpA, PavA, nanA, eno and psrP genes. Hence, this study indicates that, with such genotypic distribution pattern, Streptococcus pneumoniae from laboratory strains is a less pathogenicity as only four virulence genes detected compared with virulence gene's presence in the clinical strains. The application of real-time PCR proved a rapid and specific method for the detection of virulence genes in Streptococcus pneumoniae.
format	Thesis
qualification_level	Bachelor degree
author	Nor Azlan, Yuhaniz Atiqah
author_facet	Nor Azlan, Yuhaniz Atiqah
author_sort	Nor Azlan, Yuhaniz Atiqah
title	Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan
title_short	Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan
title_full	Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan
title_fullStr	Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan
title_full_unstemmed	Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan
title_sort	molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / yuhaniz atiqah nor azlan
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/28068/1/TD_YUHANIZ%20ATIQAH%20NOR%20AZLAN%20HS%2015_5.pdf
_version_	1783734047023824896

Molecular identification of virulence genes in laboratory strains streptococcus pneumoniae / Yuhaniz Atiqah Nor Azlan

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