Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi

L-glutaminase was found as an effective enzyme in medical and food industry especially for cancer therapy and flavor enhancing agent become a remarkable attention for seeking more information of its uses and application as well as its origin of production. Various L-gutaminase producers from differe...

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Main Author:	Rohawi, Nur Syakila
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Bacteria
Online Access:	https://ir.uitm.edu.my/id/eprint/28085/1/TD_NUR%20SYAKILA%20ROHAWI%20HS%2015_5.pdf
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id	my-uitm-ir.28085
record_format	uketd_dc
spelling	my-uitm-ir.280852020-02-03T04:29:57Z Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi 2015 Rohawi, Nur Syakila Bacteria L-glutaminase was found as an effective enzyme in medical and food industry especially for cancer therapy and flavor enhancing agent become a remarkable attention for seeking more information of its uses and application as well as its origin of production. Various L-gutaminase producers from different sources have been studied from the past years. The aim of this study is to isolate L-glutaminase producing bacteria from the red clay soil using microbiological and molecular technique. L-glutaminase producer have been isolated from rapid screening culture using submerged fermentation method. Bacteria DNA of positive L-glutaminase producer were extracted and PCR was carried out to amplify 16S rDNA gene. PCR products were sequenced and the nucleotide sequence were determine using BLAST program in NCBI website. Out of 36 bacteria isolated, 4 bacteria were positive in screening test. Result of sequencing showed Comamonas sp, Stenotrophomonas sp and Pseudomonas sp were isolated. This shows that red clay soil has been inhabitant by L-glutaminase producing bacteria. 2015 Thesis https://ir.uitm.edu.my/id/eprint/28085/ https://ir.uitm.edu.my/id/eprint/28085/1/TD_NUR%20SYAKILA%20ROHAWI%20HS%2015_5.pdf text en public degree Universiti Teknologi MARA Faculty of Health Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Bacteria
spellingShingle	Bacteria Rohawi, Nur Syakila Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi
description	L-glutaminase was found as an effective enzyme in medical and food industry especially for cancer therapy and flavor enhancing agent become a remarkable attention for seeking more information of its uses and application as well as its origin of production. Various L-gutaminase producers from different sources have been studied from the past years. The aim of this study is to isolate L-glutaminase producing bacteria from the red clay soil using microbiological and molecular technique. L-glutaminase producer have been isolated from rapid screening culture using submerged fermentation method. Bacteria DNA of positive L-glutaminase producer were extracted and PCR was carried out to amplify 16S rDNA gene. PCR products were sequenced and the nucleotide sequence were determine using BLAST program in NCBI website. Out of 36 bacteria isolated, 4 bacteria were positive in screening test. Result of sequencing showed Comamonas sp, Stenotrophomonas sp and Pseudomonas sp were isolated. This shows that red clay soil has been inhabitant by L-glutaminase producing bacteria.
format	Thesis
qualification_level	Bachelor degree
author	Rohawi, Nur Syakila
author_facet	Rohawi, Nur Syakila
author_sort	Rohawi, Nur Syakila
title	Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi
title_short	Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi
title_full	Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi
title_fullStr	Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi
title_full_unstemmed	Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi
title_sort	isolation and molecular characterization of l-glutaminase producing bacteria isolated from red clay soil in puncak alam selangor / nur syakila rohawi
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Sciences
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/28085/1/TD_NUR%20SYAKILA%20ROHAWI%20HS%2015_5.pdf
_version_	1783734049400946688

Isolation and molecular characterization of L-Glutaminase producing bacteria isolated from red clay soil in Puncak Alam Selangor / Nur Syakila Rohawi

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