The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi

The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi

Chromolaena odorata is known to possess antimicrobial effects against wide range of microorganisms including Pseudomonas aeruginosa. However, the inhibitory effects of C. odorata extracts against the biofilm growth mode of Pseudomonas aeruginosa remain uncertain. Therefore, this study was carried ou...

Full description

Saved in:
Bibliographic Details
Main Author: Wan Mohd Zawawi, Wan Mohamad Afifi
Format: Thesis
Language:English
Published: 2020
Subjects:
Microbial ecology
Online Access:https://ir.uitm.edu.my/id/eprint/32411/1/32411.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-uitm-ir.32411
record_format uketd_dc
spelling my-uitm-ir.324112024-02-23T03:46:21Z The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi 2020 Wan Mohd Zawawi, Wan Mohamad Afifi Microbial ecology Chromolaena odorata is known to possess antimicrobial effects against wide range of microorganisms including Pseudomonas aeruginosa. However, the inhibitory effects of C. odorata extracts against the biofilm growth mode of Pseudomonas aeruginosa remain uncertain. Therefore, this study was carried out to determine the antibiofilm activity of C. odorata extracts against P. aeruginosa under aerobic and anaerobic conditions. Phytochemical screening using Gas Chromatography Mass Spectrometry (GCMS) revealed the major constituents in Chromolaena odorata ethanolic extracts (COEE) as Germacrene D, Caryophyllene and δ- Cadinene. Microbroth dilution assay showed that absence of oxygen did not affect MIC and MBC of COEE against P. aeruginosa. However, antibacterial susceptibility test showed that the size of inhibition zone of COEE against P. aeruginosa were slightly different between the aerobic and anaerobic conditions. Colony forming unit counting of biofilm cells showed inhibition greater than 50% at COEE test concentration of 50mg/ml and 200mg/ml under both aerobic and anaerobic conditions. Also, COEE treatment resulted in changes in the biochemical composition of P. aeruginosa biofilm extracellular matrixes under both experimental conditions as indicated by variation in the infrared spectra in the region between 1700 and 900cm-1. A combination of SDS polyacrylamide gel electrophoresis and densitometry was used to analyze the total protein profile of P. aeruginosa biofilm under aerobic and anaerobic conditions. Treatment with COEE was found to alter the total protein profile of P. aeruginosa biofilm under aerobic and anaerobic conditions. Densitometric analysis between 400 and 750 nm demonstrated higher expression of proteins of 16 kDa, 34 kDa and 44 kDa under aerobic condition whilst lower expression of 25 kDa, 41 kDa and 55 kDa proteins was observed under anaerobic condition. Meanwhile, two dimensional polyacrylamide gel electrophoresis (2D PAGE) combined with MALDI TOF-TOF successfully identified 19 biofilm proteins under both experimental conditions. The three highest functional categories of identified P. aeruginosa biofilm proteins were found to be responsible for translation, ribosomal structure and biogenesis (56%), energy production and conversion (22%) and redox homeostasis (11%). 2020 Thesis https://ir.uitm.edu.my/id/eprint/32411/ https://ir.uitm.edu.my/id/eprint/32411/1/32411.pdf text en public masters Universiti Teknologi MARA Shah Alam Faculty of Applied Sciences Abd Hamid, Umi Marshida
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Abd Hamid, Umi Marshida
topic Microbial ecology
spellingShingle Microbial ecology
Wan Mohd Zawawi, Wan Mohamad Afifi
The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi
description Chromolaena odorata is known to possess antimicrobial effects against wide range of microorganisms including Pseudomonas aeruginosa. However, the inhibitory effects of C. odorata extracts against the biofilm growth mode of Pseudomonas aeruginosa remain uncertain. Therefore, this study was carried out to determine the antibiofilm activity of C. odorata extracts against P. aeruginosa under aerobic and anaerobic conditions. Phytochemical screening using Gas Chromatography Mass Spectrometry (GCMS) revealed the major constituents in Chromolaena odorata ethanolic extracts (COEE) as Germacrene D, Caryophyllene and δ- Cadinene. Microbroth dilution assay showed that absence of oxygen did not affect MIC and MBC of COEE against P. aeruginosa. However, antibacterial susceptibility test showed that the size of inhibition zone of COEE against P. aeruginosa were slightly different between the aerobic and anaerobic conditions. Colony forming unit counting of biofilm cells showed inhibition greater than 50% at COEE test concentration of 50mg/ml and 200mg/ml under both aerobic and anaerobic conditions. Also, COEE treatment resulted in changes in the biochemical composition of P. aeruginosa biofilm extracellular matrixes under both experimental conditions as indicated by variation in the infrared spectra in the region between 1700 and 900cm-1. A combination of SDS polyacrylamide gel electrophoresis and densitometry was used to analyze the total protein profile of P. aeruginosa biofilm under aerobic and anaerobic conditions. Treatment with COEE was found to alter the total protein profile of P. aeruginosa biofilm under aerobic and anaerobic conditions. Densitometric analysis between 400 and 750 nm demonstrated higher expression of proteins of 16 kDa, 34 kDa and 44 kDa under aerobic condition whilst lower expression of 25 kDa, 41 kDa and 55 kDa proteins was observed under anaerobic condition. Meanwhile, two dimensional polyacrylamide gel electrophoresis (2D PAGE) combined with MALDI TOF-TOF successfully identified 19 biofilm proteins under both experimental conditions. The three highest functional categories of identified P. aeruginosa biofilm proteins were found to be responsible for translation, ribosomal structure and biogenesis (56%), energy production and conversion (22%) and redox homeostasis (11%).
format Thesis
qualification_level Master's degree
author Wan Mohd Zawawi, Wan Mohamad Afifi
author_facet Wan Mohd Zawawi, Wan Mohamad Afifi
author_sort Wan Mohd Zawawi, Wan Mohamad Afifi
title The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi
title_short The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi
title_full The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi
title_fullStr The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi
title_full_unstemmed The effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / Wan Mohamad Afifi Wan Mohd Zawawi
title_sort effects of chromolaena odorata ethanolic extract on pseudomonas aeruginosa biofilm formation / wan mohamad afifi wan mohd zawawi
granting_institution Universiti Teknologi MARA Shah Alam
granting_department Faculty of Applied Sciences
publishDate 2020
url https://ir.uitm.edu.my/id/eprint/32411/1/32411.pdf
_version_ 1794191818742562816

Similar Items