Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus

In this study, Donax grandis leaves were used as a sample. The leaves were chosen as it is believed to contain high phytomedicine properties. This study was conducted to evaluate the total phenolic content, antioxidant properties and antibacterial activities that were performed against Staphylococcu...

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Main Author:	Mohd Yunus, Nur Shuhada Nabilla
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Oxidation Microbial ecology
Online Access:	https://ir.uitm.edu.my/id/eprint/32872/1/32872.pdf
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id	my-uitm-ir.32872
record_format	uketd_dc
spelling	my-uitm-ir.328722020-07-28T02:45:03Z Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus 2020-07-27 Mohd Yunus, Nur Shuhada Nabilla Oxidation Microbial ecology QV Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents In this study, Donax grandis leaves were used as a sample. The leaves were chosen as it is believed to contain high phytomedicine properties. This study was conducted to evaluate the total phenolic content, antioxidant properties and antibacterial activities that were performed against Staphylococcus aureus and Escherichia coli, a food borne bacteria. The methods that were used include the determination of total phenolic content (TPC), free radical scavenging activity of 1, 1-Diphenyl–2-Picrylhydrazyl (DPPH) and investigation of zone of inhibition against tested bacteria by using agar disc diffusion. The higher TPC depicted at 100 mg/ml with 1021.8 mg GAE/ dry weight, while the highest percent scavenging obtained through DPPH assay was 81.27% at 100 mg/ml. Thus, the higher the concentration of the Donax grandis methanolic leaves extract, the greater the potential of antioxidant activities. In the other hand, the IC50 value of the ascorbic acid was 22.55 mg/ml, whereas the IC50 value of the Donax grandis methanolic leaves extracts was 87.55 mg/ml. This shows that Donax grandis methanolic leaves extracts had an intermediate antioxidant activity compared to the standard ascorbic acid that had strong antioxidant activity. Generally, the presence of antioxidant properties in plant extract could express the potential of the extract as an antibacterial agent. Unfortunately, the zone of inhibition for antibacterial activities of Donax grandis methanolic leaves extract did not appeared, perhaps due to the concentration used in the agar disc diffusion assay was differ as used in antibiotic test. Therefore, the concentrations used were not enough to exhibit the antibacterial activities against tested bacteria. To be concluded, the effect on the antibacterial activities of Donax grandis leaves extract shows unexpected outcome against food borne bacteria in this study, but the presence of antioxidant properties in Donax grandis leaves extracts were successfully been investigated. 2020-07 Thesis https://ir.uitm.edu.my/id/eprint/32872/ https://ir.uitm.edu.my/id/eprint/32872/1/32872.pdf text en public degree Universiti Teknologi Mara Perlis Faculty of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Oxidation Microbial ecology Oxidation
spellingShingle	Oxidation Microbial ecology Oxidation Mohd Yunus, Nur Shuhada Nabilla Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus
description	In this study, Donax grandis leaves were used as a sample. The leaves were chosen as it is believed to contain high phytomedicine properties. This study was conducted to evaluate the total phenolic content, antioxidant properties and antibacterial activities that were performed against Staphylococcus aureus and Escherichia coli, a food borne bacteria. The methods that were used include the determination of total phenolic content (TPC), free radical scavenging activity of 1, 1-Diphenyl–2-Picrylhydrazyl (DPPH) and investigation of zone of inhibition against tested bacteria by using agar disc diffusion. The higher TPC depicted at 100 mg/ml with 1021.8 mg GAE/ dry weight, while the highest percent scavenging obtained through DPPH assay was 81.27% at 100 mg/ml. Thus, the higher the concentration of the Donax grandis methanolic leaves extract, the greater the potential of antioxidant activities. In the other hand, the IC50 value of the ascorbic acid was 22.55 mg/ml, whereas the IC50 value of the Donax grandis methanolic leaves extracts was 87.55 mg/ml. This shows that Donax grandis methanolic leaves extracts had an intermediate antioxidant activity compared to the standard ascorbic acid that had strong antioxidant activity. Generally, the presence of antioxidant properties in plant extract could express the potential of the extract as an antibacterial agent. Unfortunately, the zone of inhibition for antibacterial activities of Donax grandis methanolic leaves extract did not appeared, perhaps due to the concentration used in the agar disc diffusion assay was differ as used in antibiotic test. Therefore, the concentrations used were not enough to exhibit the antibacterial activities against tested bacteria. To be concluded, the effect on the antibacterial activities of Donax grandis leaves extract shows unexpected outcome against food borne bacteria in this study, but the presence of antioxidant properties in Donax grandis leaves extracts were successfully been investigated.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Yunus, Nur Shuhada Nabilla
author_facet	Mohd Yunus, Nur Shuhada Nabilla
author_sort	Mohd Yunus, Nur Shuhada Nabilla
title	Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus
title_short	Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus
title_full	Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus
title_fullStr	Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus
title_full_unstemmed	Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus
title_sort	antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / nur shuhada nabilla mohd yunus
granting_institution	Universiti Teknologi Mara Perlis
granting_department	Faculty of Applied Sciences
publishDate	2020
url	https://ir.uitm.edu.my/id/eprint/32872/1/32872.pdf
_version_	1783734187807735808

Antioxcidant and antibacterial activities of donax grandis leaves against food borne bacteria (staphylococcus aureus and escherichia coli) / Nur Shuhada Nabilla Mohd Yunus

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