Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman

The rapid emergence of various diseases caused by bacterial infections are becoming a concern and have threatened human health. The most common diseases caused by bacterial infections are skin diseases. There are many plants have been identified with the properties of antimicrobial activity that has...

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Main Author:	Kamarulzaman, Nur Syakirah
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Botanical chemistry Phytochemicals Bacteria
Online Access:	https://ir.uitm.edu.my/id/eprint/36915/1/36915.pdf
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id	my-uitm-ir.36915
record_format	uketd_dc
spelling	my-uitm-ir.369152020-11-19T02:44:35Z Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman 2020-11-16 Kamarulzaman, Nur Syakirah Botanical chemistry. Phytochemicals Bacteria The rapid emergence of various diseases caused by bacterial infections are becoming a concern and have threatened human health. The most common diseases caused by bacterial infections are skin diseases. There are many plants have been identified with the properties of antimicrobial activity that has potential to combat bacteria. The main objective of this study is to evaluate the antibacterial activity of Morinda citrifolia leaves towards Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeroginosa. Besides, the aim of this study is to identify the compounds present in Morinda citrifolia leaves by phytochemical analysis using gas chromatography and mass spectrometry (GC-MS) analysis. Another purpose of this study is to evaluate the toxicity properties of M. citrifolia leaves by using brine shrimp lethality bioassay (BSLA). Methods used for this study were plant extraction to obtain the methanolic extract of leaves, disc diffusion method to test the antibacterial activity of Morinda citrifolia leaves extract towards skin infection bacteria, Gas Chromatography-Mass Spectrometry Analysis (GC-MS) to identify the compounds present in Morinda citrifolia leaves and Brine Shrimp Lethality Bioassay (BSLA) to evaluate the toxicity properties of Morinda citrifolia leaves. The results of antibacterial activity screening have revealed that plant extract extract has antibacterial potential on all bacteria tested. GC-MS analysis has revealed major compounds such as are hydroxymethylfurfural (HMF), 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (DDMP), propanoic acid, 2-Methoxy-4-vinylphenol, oleic acid, ascorbic acid, 3-Methoxyacetophenone, 2,3,4,6-Tetramethylphenol, 2-Tetrazene,1,1-diethyl-4,4-dimethyl and sarcosine anhydride. Each compound contains beneficial properties such as antibacterial, antioxidant, anti-inflammatory, analgesic and anticancer activities. The calculated LC50 value is 583.45 μg/ml which is considered low toxic to brine shrimps. In conclusion, M. citrifolia leaves have great potential as new sources of antibacterial agent that are fundamental to combat bacteria that cause skin infections. 2020-11 Thesis https://ir.uitm.edu.my/id/eprint/36915/ https://ir.uitm.edu.my/id/eprint/36915/1/36915.pdf text en public degree Universiti Teknologi Mara Perlis Faculty Of Applied Sciences
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Botanical chemistry Phytochemicals Bacteria
spellingShingle	Botanical chemistry Phytochemicals Bacteria Kamarulzaman, Nur Syakirah Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman
description	The rapid emergence of various diseases caused by bacterial infections are becoming a concern and have threatened human health. The most common diseases caused by bacterial infections are skin diseases. There are many plants have been identified with the properties of antimicrobial activity that has potential to combat bacteria. The main objective of this study is to evaluate the antibacterial activity of Morinda citrifolia leaves towards Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeroginosa. Besides, the aim of this study is to identify the compounds present in Morinda citrifolia leaves by phytochemical analysis using gas chromatography and mass spectrometry (GC-MS) analysis. Another purpose of this study is to evaluate the toxicity properties of M. citrifolia leaves by using brine shrimp lethality bioassay (BSLA). Methods used for this study were plant extraction to obtain the methanolic extract of leaves, disc diffusion method to test the antibacterial activity of Morinda citrifolia leaves extract towards skin infection bacteria, Gas Chromatography-Mass Spectrometry Analysis (GC-MS) to identify the compounds present in Morinda citrifolia leaves and Brine Shrimp Lethality Bioassay (BSLA) to evaluate the toxicity properties of Morinda citrifolia leaves. The results of antibacterial activity screening have revealed that plant extract extract has antibacterial potential on all bacteria tested. GC-MS analysis has revealed major compounds such as are hydroxymethylfurfural (HMF), 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (DDMP), propanoic acid, 2-Methoxy-4-vinylphenol, oleic acid, ascorbic acid, 3-Methoxyacetophenone, 2,3,4,6-Tetramethylphenol, 2-Tetrazene,1,1-diethyl-4,4-dimethyl and sarcosine anhydride. Each compound contains beneficial properties such as antibacterial, antioxidant, anti-inflammatory, analgesic and anticancer activities. The calculated LC50 value is 583.45 μg/ml which is considered low toxic to brine shrimps. In conclusion, M. citrifolia leaves have great potential as new sources of antibacterial agent that are fundamental to combat bacteria that cause skin infections.
format	Thesis
qualification_level	Bachelor degree
author	Kamarulzaman, Nur Syakirah
author_facet	Kamarulzaman, Nur Syakirah
author_sort	Kamarulzaman, Nur Syakirah
title	Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman
title_short	Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman
title_full	Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman
title_fullStr	Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman
title_full_unstemmed	Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman
title_sort	phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / nur syakirah kamarulzaman
granting_institution	Universiti Teknologi Mara Perlis
granting_department	Faculty Of Applied Sciences
publishDate	2020
url	https://ir.uitm.edu.my/id/eprint/36915/1/36915.pdf
_version_	1783734374315851776

Phytochemical analysis, toxicity and antibacterial activities of morinda citrifolia leaves / Nur Syakirah Kamarulzaman

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