Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim

Aquilaria malaccensis Lamk. belongs to the Thymelaeaceae family and known as agarwood in the world and Gaharu in Malaysia, that is commercially identified as a potential aromatic plant. Inoculation for resinous compounds in A. malaccensis naturally is difficult. Hence, an artificial inoculation via...

Full description

Saved in:

Bibliographic Details
Main Author:	Ibrahim, Nordiana Najwa
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Seeds Seed technology Ficus
Online Access:	https://ir.uitm.edu.my/id/eprint/37181/1/37181.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.37181
record_format	uketd_dc
spelling	my-uitm-ir.371812022-11-01T09:07:41Z Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim 2016 Ibrahim, Nordiana Najwa Seeds. Seed technology Ficus Aquilaria malaccensis Lamk. belongs to the Thymelaeaceae family and known as agarwood in the world and Gaharu in Malaysia, that is commercially identified as a potential aromatic plant. Inoculation for resinous compounds in A. malaccensis naturally is difficult. Hence, an artificial inoculation via in vitro inoculation method was developed. Stem explants of A. malaccensis were cultured on MS media supplemented with 1.0 mg/L BAP and 0.1 mg/L 2,4-D for the callus induction. Embryogenic callus was successfully initiated on MS media supplemented with 1.0 mg/L and 0.1 mg/L 2,4-D added with 500 mg/L casein hydrolysate by producing globular, heart-shaped and torpedo stages. Addition of additive including casein hydrolysate added with 10.0 mg/L absicic acid (ABA) give a significance effect on the development and maturation of the embryogenic callus. Therefore, induction of a high frequency of somatic embryogenesis in stem explants on MS media is possible. Optimum callus formation from MS media added with 500 mg/L casein hydrolysate and 10.0 mg/L ABA which was used to induce resin of A. malaccensis through in vitro artificial inoculation. The inoculant known as 4 Moon Booster which was inoculated into MS media then the interaction of the inoculant to embryogenic callus and media was successfully obtained. The result revealed that, embryogenic callus turned into dark brown from creamy white color and give an odour. In addition, the inoculated media were also turned to brown and produced a sense of sensory when the container cap is opened. So, the inoculant gives significance artificial inoculation effect on the embryogenic callus and to the media. A reliable protocol useful for A. malaccensis through somatic embryogenesis was successfully established for artificial inoculation purposes. 2016 Thesis https://ir.uitm.edu.my/id/eprint/37181/ https://ir.uitm.edu.my/id/eprint/37181/1/37181.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Awal, Asmah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Awal, Asmah
topic	Seeds Seed technology Ficus
spellingShingle	Seeds Seed technology Ficus Ibrahim, Nordiana Najwa Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim
description	Aquilaria malaccensis Lamk. belongs to the Thymelaeaceae family and known as agarwood in the world and Gaharu in Malaysia, that is commercially identified as a potential aromatic plant. Inoculation for resinous compounds in A. malaccensis naturally is difficult. Hence, an artificial inoculation via in vitro inoculation method was developed. Stem explants of A. malaccensis were cultured on MS media supplemented with 1.0 mg/L BAP and 0.1 mg/L 2,4-D for the callus induction. Embryogenic callus was successfully initiated on MS media supplemented with 1.0 mg/L and 0.1 mg/L 2,4-D added with 500 mg/L casein hydrolysate by producing globular, heart-shaped and torpedo stages. Addition of additive including casein hydrolysate added with 10.0 mg/L absicic acid (ABA) give a significance effect on the development and maturation of the embryogenic callus. Therefore, induction of a high frequency of somatic embryogenesis in stem explants on MS media is possible. Optimum callus formation from MS media added with 500 mg/L casein hydrolysate and 10.0 mg/L ABA which was used to induce resin of A. malaccensis through in vitro artificial inoculation. The inoculant known as 4 Moon Booster which was inoculated into MS media then the interaction of the inoculant to embryogenic callus and media was successfully obtained. The result revealed that, embryogenic callus turned into dark brown from creamy white color and give an odour. In addition, the inoculated media were also turned to brown and produced a sense of sensory when the container cap is opened. So, the inoculant gives significance artificial inoculation effect on the embryogenic callus and to the media. A reliable protocol useful for A. malaccensis through somatic embryogenesis was successfully established for artificial inoculation purposes.
format	Thesis
qualification_level	Master's degree
author	Ibrahim, Nordiana Najwa
author_facet	Ibrahim, Nordiana Najwa
author_sort	Ibrahim, Nordiana Najwa
title	Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim
title_short	Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim
title_full	Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim
title_fullStr	Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim
title_full_unstemmed	Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim
title_sort	protocol of somatic embryo towards in vitro artificial inoculation on aquilaria malaccensis lamk. (gaharu) / nordiana najwa ibrahim
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/37181/1/37181.pdf
_version_	1783734395608236032

Protocol of somatic embryo towards in vitro artificial inoculation on Aquilaria malaccensis Lamk. (gaharu) / Nordiana Najwa Ibrahim

Similar Items