In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril

Neuroinflammation and oxidative stress are major factors of neurodegenerative damage correlated to cognitive impairment in Alzheimer's disease (AD). It results primarily from overproduction of oxidative stress that induce activation of pro-inflammatory cytokines causing to neuronal death. This...

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Main Author:	Jusril, Nor Atiqah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Medical education Medical schools Research
Online Access:	https://ir.uitm.edu.my/id/eprint/37204/1/37204.pdf
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spelling	my-uitm-ir.372042022-11-01T09:13:48Z In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril 2018 Jusril, Nor Atiqah Medical education. Medical schools. Research Herbs. Herbal teas Neuroinflammation and oxidative stress are major factors of neurodegenerative damage correlated to cognitive impairment in Alzheimer's disease (AD). It results primarily from overproduction of oxidative stress that induce activation of pro-inflammatory cytokines causing to neuronal death. This study investigated the anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar added with Centella asiatica in SH-SY5Y neuroblastoma cells. The neuroprotective effect of apple or date vinegar added with various percentage of C. asiatica (0, 0.5, 2, 5%) was determined in vitro. The methanolic extract of apple vinegar added with C. asiatica [AV-CA (2%)] and date vinegar added with C. asiatica [DV-CA (2%)] extracts showed potent neuroprotective effect. Both extracts were subjected to liquid-liquid partitioning yielded aqueous (H20: AV-CA/DV-CA) and ethyl acetate (EA: AV-CA/DV-CA) at [2% CA] extracts. All of extracts were tested for anti-inflammatory, antioxidant, and anti- acetylcholinesterase activities. Anti-inflammatory response against nitric oxide (NO) was measured in SH-SY5Y neuroblastoma cells. Meanwhile, the intercellular reactive oxygen species ROS inhibition was measured as antioxidant activity and percentage inhibition of acetylcholinesterase (AChE) was measured by using commercially available kit's test. In general, all extracts significantly (P < 0.05) inhibited NO production without affecting cell viability. EA-DVCA extract showed better NO production inhibition and induced more attenuation effect on nitrite production with an IC50 value of 563.5 ±0.13 ug/mL. Furthermore, EA: AV-CA extract showed a high percentage ROS inhibition with an IC50 value of 17.54 ± 0.40 ug/mL. Strongest AChE inhibition activity was observed in EA: DV-CA (2%) with (IC50 = 9.087 ug/mL. It is suggested that the ameliorating effect of EA: DV-CA (2%) on LPSinduced neuroinflammation and memory deficit seems to be attenuated via antiinflammatory, inhibition of reactive oxygen species and AChE activities. 2018 Thesis https://ir.uitm.edu.my/id/eprint/37204/ https://ir.uitm.edu.my/id/eprint/37204/1/37204.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Adenan, Mohd Ilham
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Adenan, Mohd Ilham
topic	Medical education Medical schools Research Medical education Medical schools Research
spellingShingle	Medical education Medical schools Research Medical education Medical schools Research Jusril, Nor Atiqah In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril
description	Neuroinflammation and oxidative stress are major factors of neurodegenerative damage correlated to cognitive impairment in Alzheimer's disease (AD). It results primarily from overproduction of oxidative stress that induce activation of pro-inflammatory cytokines causing to neuronal death. This study investigated the anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar added with Centella asiatica in SH-SY5Y neuroblastoma cells. The neuroprotective effect of apple or date vinegar added with various percentage of C. asiatica (0, 0.5, 2, 5%) was determined in vitro. The methanolic extract of apple vinegar added with C. asiatica [AV-CA (2%)] and date vinegar added with C. asiatica [DV-CA (2%)] extracts showed potent neuroprotective effect. Both extracts were subjected to liquid-liquid partitioning yielded aqueous (H20: AV-CA/DV-CA) and ethyl acetate (EA: AV-CA/DV-CA) at [2% CA] extracts. All of extracts were tested for anti-inflammatory, antioxidant, and anti- acetylcholinesterase activities. Anti-inflammatory response against nitric oxide (NO) was measured in SH-SY5Y neuroblastoma cells. Meanwhile, the intercellular reactive oxygen species ROS inhibition was measured as antioxidant activity and percentage inhibition of acetylcholinesterase (AChE) was measured by using commercially available kit's test. In general, all extracts significantly (P < 0.05) inhibited NO production without affecting cell viability. EA-DVCA extract showed better NO production inhibition and induced more attenuation effect on nitrite production with an IC50 value of 563.5 ±0.13 ug/mL. Furthermore, EA: AV-CA extract showed a high percentage ROS inhibition with an IC50 value of 17.54 ± 0.40 ug/mL. Strongest AChE inhibition activity was observed in EA: DV-CA (2%) with (IC50 = 9.087 ug/mL. It is suggested that the ameliorating effect of EA: DV-CA (2%) on LPSinduced neuroinflammation and memory deficit seems to be attenuated via antiinflammatory, inhibition of reactive oxygen species and AChE activities.
format	Thesis
qualification_level	Master's degree
author	Jusril, Nor Atiqah
author_facet	Jusril, Nor Atiqah
author_sort	Jusril, Nor Atiqah
title	In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril
title_short	In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril
title_full	In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril
title_fullStr	In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril
title_full_unstemmed	In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril
title_sort	in vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / nor atiqah jusril
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/37204/1/37204.pdf
_version_	1783734400659226624

In vitro evaluation of anti-inflammatory, antioxidant, and anti-acetylcholinesterase activities of apple and date vinegar extracts added with centella asiatica / Nor Atiqah Jusril

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