Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf

Polyhydroxyalkanoates (PHA) are bioplastics, produced by various bacteria as food and energy reservoir. PHA is an alternative for synthetic plastic because they are environmentally friendly and can be degraded naturally by microorganisms. One of the important factors for the growth of PHA producing...

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Main Author:	Mohamad Yusuf, Nurfarahana Jasmin
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Environmental protection Sanitary landfills
Online Access:	https://ir.uitm.edu.my/id/eprint/37207/1/37207.pdf
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spelling	my-uitm-ir.372072022-11-01T09:19:34Z Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf 2018 Mohamad Yusuf, Nurfarahana Jasmin Environmental protection Sanitary landfills Polyhydroxyalkanoates (PHA) are bioplastics, produced by various bacteria as food and energy reservoir. PHA is an alternative for synthetic plastic because they are environmentally friendly and can be degraded naturally by microorganisms. One of the important factors for the growth of PHA producing bacteria is an excess of carbon supply. In order to reduce the overall cost of PHA production, a low cost pure substrate, which is cocoa pod husks (CPH) was used as a carbon source. The objectives of this study were to isolate and screen PHA producing bacteria from landfill samples which are leachate and soil, to identify the PHA producing bacteria by using morphological characterization and 16s rDNA gene sequencing and to determine the best percentage of CPH that can be used as a carbon source for PHA producing bacteria. PHA producing bacteria from leachate and soil from landfill in Jeram, Selangor were screened by using Nile Blue A staining method. Two potential PHA producers with the brightest fluorescence under UV light from each samples were isolated and characterized by using morphological and molecular identification. Results of morphological identification shows all bacterial isolates have a rod shape and have a capsule, three bacterial isolates have an endospore while the remaining does not have endospore. Three out of four were Gram positive bacteria and the remaining was Gram negative bacteria. These isolates were confirmed of their identity as K. pneumoniae, B. cereus and B. toyonenis using 16s rDNA gene sequencing. Different concentration of CPH, which are 2% (w/v), 5% (w/v), 7% (w/v) and 10% (w/v) were used to study the best percentage of CPH that can be used as carbon source. PHA accumulation was the highest at 7% (w/v) for all bacterial species tested and lowest at 10% (w/v) CPH except for B. toyonensis. 2018 Thesis https://ir.uitm.edu.my/id/eprint/37207/ https://ir.uitm.edu.my/id/eprint/37207/1/37207.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Wan Abdul Razak, Wan Razarinah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Wan Abdul Razak, Wan Razarinah
topic	Environmental protection Sanitary landfills
spellingShingle	Environmental protection Sanitary landfills Mohamad Yusuf, Nurfarahana Jasmin Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf
description	Polyhydroxyalkanoates (PHA) are bioplastics, produced by various bacteria as food and energy reservoir. PHA is an alternative for synthetic plastic because they are environmentally friendly and can be degraded naturally by microorganisms. One of the important factors for the growth of PHA producing bacteria is an excess of carbon supply. In order to reduce the overall cost of PHA production, a low cost pure substrate, which is cocoa pod husks (CPH) was used as a carbon source. The objectives of this study were to isolate and screen PHA producing bacteria from landfill samples which are leachate and soil, to identify the PHA producing bacteria by using morphological characterization and 16s rDNA gene sequencing and to determine the best percentage of CPH that can be used as a carbon source for PHA producing bacteria. PHA producing bacteria from leachate and soil from landfill in Jeram, Selangor were screened by using Nile Blue A staining method. Two potential PHA producers with the brightest fluorescence under UV light from each samples were isolated and characterized by using morphological and molecular identification. Results of morphological identification shows all bacterial isolates have a rod shape and have a capsule, three bacterial isolates have an endospore while the remaining does not have endospore. Three out of four were Gram positive bacteria and the remaining was Gram negative bacteria. These isolates were confirmed of their identity as K. pneumoniae, B. cereus and B. toyonenis using 16s rDNA gene sequencing. Different concentration of CPH, which are 2% (w/v), 5% (w/v), 7% (w/v) and 10% (w/v) were used to study the best percentage of CPH that can be used as carbon source. PHA accumulation was the highest at 7% (w/v) for all bacterial species tested and lowest at 10% (w/v) CPH except for B. toyonensis.
format	Thesis
qualification_level	Master's degree
author	Mohamad Yusuf, Nurfarahana Jasmin
author_facet	Mohamad Yusuf, Nurfarahana Jasmin
author_sort	Mohamad Yusuf, Nurfarahana Jasmin
title	Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf
title_short	Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf
title_full	Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf
title_fullStr	Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf
title_full_unstemmed	Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf
title_sort	screening and isolation of polyhydroxyalkanoates (pha)-producing bacteria from landfill by using cocoa pod husks as carbon source / nurfarahana jasmin mohamad yusuf
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2018
url	https://ir.uitm.edu.my/id/eprint/37207/1/37207.pdf
_version_	1783734401149960192

Screening and isolation of polyhydroxyalkanoates (PHA)-producing bacteria from landfill by using cocoa POD husks as carbon source / Nurfarahana Jasmin Mohamad Yusuf

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