Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis

Cryopreservation is widely applied by researchers to preserve genetic materials of a sample under a very low temperature using liquid nitrogen. Rice is possible to be stored and preserved under a very low temperature with the presence of cryoprotectants as to maintain cellular processes of the cells...

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Main Author:	Mohamad Napis, Nur Syamimi
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Genetic engineering applications
Online Access:	https://ir.uitm.edu.my/id/eprint/37297/1/37297.pdf
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id	my-uitm-ir.37297
record_format	uketd_dc
spelling	my-uitm-ir.372972023-08-21T01:59:58Z Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis 2017 Mohamad Napis, Nur Syamimi Genetic engineering applications Cryopreservation is widely applied by researchers to preserve genetic materials of a sample under a very low temperature using liquid nitrogen. Rice is possible to be stored and preserved under a very low temperature with the presence of cryoprotectants as to maintain cellular processes of the cells after cryopreservation. Two cryopreservation protocols which are dehydration and encapsulation-dehydration were used in this study to preserve genetic materials of an indica variety of MR 219 rice. The sterilised seeds of the MR 219 rice variety were cultured on MSO for four weeks to get aseptic seedlings as source of stem explants. Stem explants were, then cultured on MSO for two weeks. The stems (5 mm in length) were excised from 2-week-old plantlet derived from stem explants that were cultured on MSO medium. For dehydration method, stems were dehydrated for 219 minutes in a sterile Petri dish with 3.0 g silica gel to allow only 20 percent of water contained in the stems. O 2017 Thesis https://ir.uitm.edu.my/id/eprint/37297/ https://ir.uitm.edu.my/id/eprint/37297/1/37297.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Mohd, Farida Zuraina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohd, Farida Zuraina
topic	Genetic engineering applications
spellingShingle	Genetic engineering applications Mohamad Napis, Nur Syamimi Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis
description	Cryopreservation is widely applied by researchers to preserve genetic materials of a sample under a very low temperature using liquid nitrogen. Rice is possible to be stored and preserved under a very low temperature with the presence of cryoprotectants as to maintain cellular processes of the cells after cryopreservation. Two cryopreservation protocols which are dehydration and encapsulation-dehydration were used in this study to preserve genetic materials of an indica variety of MR 219 rice. The sterilised seeds of the MR 219 rice variety were cultured on MSO for four weeks to get aseptic seedlings as source of stem explants. Stem explants were, then cultured on MSO for two weeks. The stems (5 mm in length) were excised from 2-week-old plantlet derived from stem explants that were cultured on MSO medium. For dehydration method, stems were dehydrated for 219 minutes in a sterile Petri dish with 3.0 g silica gel to allow only 20 percent of water contained in the stems. O
format	Thesis
qualification_level	Master's degree
author	Mohamad Napis, Nur Syamimi
author_facet	Mohamad Napis, Nur Syamimi
author_sort	Mohamad Napis, Nur Syamimi
title	Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis
title_short	Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis
title_full	Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis
title_fullStr	Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis
title_full_unstemmed	Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis
title_sort	genetic stability of cryopreserved mr 219 rice variety revealed by inter-retrotransposon amplified polymorphism (irap) / nur syamimi mohamad napis
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/37297/1/37297.pdf
_version_	1783734411794055168

Genetic stability of cryopreserved MR 219 rice variety revealed by inter-retrotransposon amplified polymorphism (IRAP) / Nur Syamimi Mohamad Napis

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