Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir

Medicinal mushrooms are well-known for nutritional benefits. However, it is necessary to evaluate the toxicity of medicinal mushrooms. The aim of this study was to identify the potential toxicity of Agaricus blazei, Grifola frondosa and Hericium erinaceus. In this study, screening of phytochemical c...

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Main Author:	Nasir, Nur Shahirah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Individual drugs and other agents Medicinal plants (Culture only)
Online Access:	https://ir.uitm.edu.my/id/eprint/37323/1/37323.pdf
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id	my-uitm-ir.37323
record_format	uketd_dc
spelling	my-uitm-ir.373232022-11-01T08:24:54Z Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir 2017 Nasir, Nur Shahirah Individual drugs and other agents Medicinal plants (Culture only) Medicinal mushrooms are well-known for nutritional benefits. However, it is necessary to evaluate the toxicity of medicinal mushrooms. The aim of this study was to identify the potential toxicity of Agaricus blazei, Grifola frondosa and Hericium erinaceus. In this study, screening of phytochemical compound, evaluating the cytotoxicity effect (MTT assay), determining the mode of cell death (Annexin V), observing the morphological changes of cell and analysing the genotoxicity (Comet Asssay) were done to assess the potential toxicity. All toxicity tests were evaluated against Chinese hamster lung fibroblast cell line (V79) using methanol and aqueous of the extracts at 0.125 mg/ml, 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml and 2 mg/ml. Phytochemical screening showed the extracts contain abundantly of alkaloid. The MTT assay demonstrated that all extracts showed low cytotoxicity effect at 2 mg/ml. Only aqueous extract of A. blazei displayed the highest cytotoxicity effect at 1.7 mg/ml (IC50). The Mode of Cell Death for viable V79 cells for A. blazei was higher at 2 mg/ml (93.9% ±0.721) compared to apoptosis and necrosis using Annexin V. Cell morphology did not show any sign of apoptotic and cell injury at 1 mg/ml and 2 mg/ml of mushroom extract during 24 hours treatment. Lastly, genotoxicity activity for A. blazei, G. frondosa and H. erinaceus in V79 cell results revealed that DNA damages were detected at 1 mg/ml and 2 mg/ml. In conclusion, these three selected medicinal mushrooms had caused toxicity to normal cells as demonstrated by V79 cell line at 1 to 2 mg/ml. 2017 Thesis https://ir.uitm.edu.my/id/eprint/37323/ https://ir.uitm.edu.my/id/eprint/37323/1/37323.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Mohd Yusof, Farida Zuraina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohd Yusof, Farida Zuraina
topic	Individual drugs and other agents Medicinal plants (Culture only)
spellingShingle	Individual drugs and other agents Medicinal plants (Culture only) Nasir, Nur Shahirah Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
description	Medicinal mushrooms are well-known for nutritional benefits. However, it is necessary to evaluate the toxicity of medicinal mushrooms. The aim of this study was to identify the potential toxicity of Agaricus blazei, Grifola frondosa and Hericium erinaceus. In this study, screening of phytochemical compound, evaluating the cytotoxicity effect (MTT assay), determining the mode of cell death (Annexin V), observing the morphological changes of cell and analysing the genotoxicity (Comet Asssay) were done to assess the potential toxicity. All toxicity tests were evaluated against Chinese hamster lung fibroblast cell line (V79) using methanol and aqueous of the extracts at 0.125 mg/ml, 0.25 mg/ml, 0.5 mg/ml, 1 mg/ml and 2 mg/ml. Phytochemical screening showed the extracts contain abundantly of alkaloid. The MTT assay demonstrated that all extracts showed low cytotoxicity effect at 2 mg/ml. Only aqueous extract of A. blazei displayed the highest cytotoxicity effect at 1.7 mg/ml (IC50). The Mode of Cell Death for viable V79 cells for A. blazei was higher at 2 mg/ml (93.9% ±0.721) compared to apoptosis and necrosis using Annexin V. Cell morphology did not show any sign of apoptotic and cell injury at 1 mg/ml and 2 mg/ml of mushroom extract during 24 hours treatment. Lastly, genotoxicity activity for A. blazei, G. frondosa and H. erinaceus in V79 cell results revealed that DNA damages were detected at 1 mg/ml and 2 mg/ml. In conclusion, these three selected medicinal mushrooms had caused toxicity to normal cells as demonstrated by V79 cell line at 1 to 2 mg/ml.
format	Thesis
qualification_level	Master's degree
author	Nasir, Nur Shahirah
author_facet	Nasir, Nur Shahirah
author_sort	Nasir, Nur Shahirah
title	Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_short	Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_full	Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_fullStr	Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_full_unstemmed	Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir
title_sort	cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using chinese hamster lung fibroblast cell line (v79) / nur shahirah nasir
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/37323/1/37323.pdf
_version_	1783734416103702528

Cytotoxicity and genotoxicity studies of selected medicinal mushrooms extracts using Chinese hamster lung fibroblast cell line (V79) / Nur Shahirah Nasir

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