Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir

Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir

Chirality is a chemical property that is very important to current pharmaceutical development processes. This study explores the employment of circular dichroism (CD) spectroscopy as a powerful tool for the absolute configuration (AC) assignment of labdane diterpenes which are considered as flexible...

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Main Author: A Kadir, Muhamad Faid
Format: Thesis
Language:English
Published: 2018
Online Access:https://ir.uitm.edu.my/id/eprint/38255/1/38255.pdf
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spelling my-uitm-ir.382552023-01-26T09:38:07Z Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir 2018 A Kadir, Muhamad Faid Chirality is a chemical property that is very important to current pharmaceutical development processes. This study explores the employment of circular dichroism (CD) spectroscopy as a powerful tool for the absolute configuration (AC) assignment of labdane diterpenes which are considered as flexible chiral molecules. Andrographis paniculata (Burm. f.) Wall, ex Nees is a medicinal plant whose primary constituent is the labdane diterpene, andrographolide. The first objective of this study was to isolate and characterize andrographolide as the major constituent from A. paniculata using various chromatographic and spectroscopic methods. Upon the determination of relative configuration of andrographolide, the second objective was to measure the CD properties of andrographolide via experimental ECD and VCD techniques in solution form. The last objective was to determine the AC of andrographolide by the comparison of experimental ECD and VCD spectra to simulated data using TDDFT calculation. The functional theory employed was CAM-B3LYP/6-31+G(d,p) level as implemented in Gaussian 09W software package. From this study, andrographolide (1) was successfully isolated and characterized and its initial relative configuration was determined by ID and 2D NMR spectroscopy. Comparison of the experimental and simulated ECD and VCD data of andrographolide resulted in very good agreement qualitatively leading to the establishment of its absolute configuration (in solution state) as 3R, 4R, 5S, 9R, 10R, and 14S. In conclusion, this work clearly supports the use of CD techniques for the AC determination of naturally occurring labdane-type diterpenes and it may be applied to other structurally complex and flexible natural products. A concerted application of both ECD and VCD techniques should be considered as the main and preferable approach over other chiroptical methods. 2018 Thesis https://ir.uitm.edu.my/id/eprint/38255/ https://ir.uitm.edu.my/id/eprint/38255/1/38255.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Ahmad, Rohaya
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Ahmad, Rohaya
description Chirality is a chemical property that is very important to current pharmaceutical development processes. This study explores the employment of circular dichroism (CD) spectroscopy as a powerful tool for the absolute configuration (AC) assignment of labdane diterpenes which are considered as flexible chiral molecules. Andrographis paniculata (Burm. f.) Wall, ex Nees is a medicinal plant whose primary constituent is the labdane diterpene, andrographolide. The first objective of this study was to isolate and characterize andrographolide as the major constituent from A. paniculata using various chromatographic and spectroscopic methods. Upon the determination of relative configuration of andrographolide, the second objective was to measure the CD properties of andrographolide via experimental ECD and VCD techniques in solution form. The last objective was to determine the AC of andrographolide by the comparison of experimental ECD and VCD spectra to simulated data using TDDFT calculation. The functional theory employed was CAM-B3LYP/6-31+G(d,p) level as implemented in Gaussian 09W software package. From this study, andrographolide (1) was successfully isolated and characterized and its initial relative configuration was determined by ID and 2D NMR spectroscopy. Comparison of the experimental and simulated ECD and VCD data of andrographolide resulted in very good agreement qualitatively leading to the establishment of its absolute configuration (in solution state) as 3R, 4R, 5S, 9R, 10R, and 14S. In conclusion, this work clearly supports the use of CD techniques for the AC determination of naturally occurring labdane-type diterpenes and it may be applied to other structurally complex and flexible natural products. A concerted application of both ECD and VCD techniques should be considered as the main and preferable approach over other chiroptical methods.
format Thesis
qualification_level Master's degree
author A Kadir, Muhamad Faid
spellingShingle A Kadir, Muhamad Faid
Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir
author_facet A Kadir, Muhamad Faid
author_sort A Kadir, Muhamad Faid
title Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir
title_short Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir
title_full Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir
title_fullStr Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir
title_full_unstemmed Absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / Muhamad Faid A Kadir
title_sort absolute configuration determination of andrographolide via experimental and theoretical circular dichroism spectroscopic techniques / muhamad faid a kadir
granting_institution Universiti Teknologi MARA (UiTM)
granting_department Faculty of Applied Sciences
publishDate 2018
url https://ir.uitm.edu.my/id/eprint/38255/1/38255.pdf
_version_ 1783734460465807360

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