Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz

Staphylococcus aureus (S. aureus) is a normal flora of the nostrils in healthy population and most healthy carriers have S. aureus on their skin without showing any active infection. About 30% of human population carries S. aureus in their nose, which is an important risk for nosocomial and communit...

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Main Author:	Abdul Aziz, Iman
Format:	Thesis
Language:	English
Published:	2016
Subjects:	Medical education Medical schools Research Pharmaceutical technology
Online Access:	https://ir.uitm.edu.my/id/eprint/38710/1/38710.pdf
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id	my-uitm-ir.38710
record_format	uketd_dc
spelling	my-uitm-ir.387102022-07-05T07:41:46Z Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz 2016 Abdul Aziz, Iman Medical education. Medical schools. Research Pharmaceutical technology Staphylococcus aureus (S. aureus) is a normal flora of the nostrils in healthy population and most healthy carriers have S. aureus on their skin without showing any active infection. About 30% of human population carries S. aureus in their nose, which is an important risk for nosocomial and community-acquired infections worldwide. As Medical Laboratory Technology (MLT) students will be interacting and exposed to hospital environments in the future, they may be the potential nasal carriers for spreading the organism to hospital patients. This cross-sectional study involving 74 pre-clinical and 70 clinical students aims to determine the prevalence of S. aureus from nasal isolates among MLT students in UiTM Puncak Alam, classify these students into persistent, intermittent or non-carriers, and evaluate the association between S. aureus nasal colonization and possible risk factors in this population. After signing an informed consent, students completed a Survey Form related to possible risk factors for colonization, and nasal swabs were collected. S. aureus isolated undergo further reconfirmation by the amplification of by real-time Polymerase Chain Reaction (RT-PCR) to determine the presence of nuc gene. To determine the carriage types, each student were subjected to two additional nasal swabs collection, each taken three weeks apart. From 144 nasal swabs collected, 18 (12.5%) were identified as S. aureus carriers. Ten (6.9%) were persistent carriers, 8 (5.6%o) were intermittent carriers and 126 (87.5%) were non-carries. There were no statistical significant association between S. aureus nasal carrier status and gender, race, clinical status, and medical and hygienic factors (p>0.05). In conclusion, only small percentage of MLT students were identified as intermittent or persistent carriers of S. aureus nasal carrier while the majority were non-carrier. 2016 Thesis https://ir.uitm.edu.my/id/eprint/38710/ https://ir.uitm.edu.my/id/eprint/38710/1/38710.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Mastuki, Mohd Fahmi
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mastuki, Mohd Fahmi
topic	Medical education Medical schools Research Pharmaceutical technology
spellingShingle	Medical education Medical schools Research Pharmaceutical technology Abdul Aziz, Iman Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
description	Staphylococcus aureus (S. aureus) is a normal flora of the nostrils in healthy population and most healthy carriers have S. aureus on their skin without showing any active infection. About 30% of human population carries S. aureus in their nose, which is an important risk for nosocomial and community-acquired infections worldwide. As Medical Laboratory Technology (MLT) students will be interacting and exposed to hospital environments in the future, they may be the potential nasal carriers for spreading the organism to hospital patients. This cross-sectional study involving 74 pre-clinical and 70 clinical students aims to determine the prevalence of S. aureus from nasal isolates among MLT students in UiTM Puncak Alam, classify these students into persistent, intermittent or non-carriers, and evaluate the association between S. aureus nasal colonization and possible risk factors in this population. After signing an informed consent, students completed a Survey Form related to possible risk factors for colonization, and nasal swabs were collected. S. aureus isolated undergo further reconfirmation by the amplification of by real-time Polymerase Chain Reaction (RT-PCR) to determine the presence of nuc gene. To determine the carriage types, each student were subjected to two additional nasal swabs collection, each taken three weeks apart. From 144 nasal swabs collected, 18 (12.5%) were identified as S. aureus carriers. Ten (6.9%) were persistent carriers, 8 (5.6%o) were intermittent carriers and 126 (87.5%) were non-carries. There were no statistical significant association between S. aureus nasal carrier status and gender, race, clinical status, and medical and hygienic factors (p>0.05). In conclusion, only small percentage of MLT students were identified as intermittent or persistent carriers of S. aureus nasal carrier while the majority were non-carrier.
format	Thesis
qualification_level	Bachelor degree
author	Abdul Aziz, Iman
author_facet	Abdul Aziz, Iman
author_sort	Abdul Aziz, Iman
title	Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_short	Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_full	Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_fullStr	Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_full_unstemmed	Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz
title_sort	molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in uitm puncak alam / iman abdul aziz
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2016
url	https://ir.uitm.edu.my/id/eprint/38710/1/38710.pdf
_version_	1783734480765190144

Molecular identification and carriage pattern of staphylococcus aureus from nasal isolates among medical laboratory technology students in UiTM Puncak Alam / Iman Abdul Aziz

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