Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady

Alzheimer's disease (AD) is characterized by loss of memory and deterioration of cognitive function. To date, AD treatment remains a great challenge as pathogenesis of this disease is still poorly understood. Reliable AD biomarkers would therefore be of high relevance, not only to assist early...

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Main Author:	Enche Ady, Che Nor Adlia
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Diseases of the circulatory (Cardiovascular) system Diseases of the musculoskeletal system
Online Access:	https://ir.uitm.edu.my/id/eprint/38869/1/38869.pdf
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spelling	my-uitm-ir.388692021-08-29T12:09:21Z Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady 2017-09 Enche Ady, Che Nor Adlia Diseases of the circulatory (Cardiovascular) system Diseases of the musculoskeletal system Alzheimer's disease (AD) is characterized by loss of memory and deterioration of cognitive function. To date, AD treatment remains a great challenge as pathogenesis of this disease is still poorly understood. Reliable AD biomarkers would therefore be of high relevance, not only to assist early detection but also to uncover potential drug targets that can be manipulated to slow down disease progression. This study compared metabolomic profiles of blood serum from AD patients to those of the non-demented controls. Qualitative metabolomic profiles of blood serum were acquired using the liquid chromatography/mass spectrometry quadrupole time-of-flight (LC-MSQTOF) and Nuclear Magnetic Resonance (NMR). The most important finding that emerged from this study is that lipids and amino acids are responsible for the metabolic changes linked to AD. The present study has revealed four LC-MS-derived metabolites [(N-(2- hydroxyethyl)palmita ide, N-(2-hydroxyethyl)icosanamide, dihydroceramide, Phyto sphingosine] and four NMR-derived metabolites (isoleucine, creatinine, VLDL, lipid) that might be implicated in AD pathogenesis. These metabolites panel may serve as potential biomarkers which are associated with neuronal P amyloid release, apoptosis, dysregulated energy metabolism and brain oxidative impairment that will lead to neuronal cell death. In terms of pathways, sphingolipid metabolism and biosynthesis of valine, leucine and isoleucine were found to be perturbed in AD. Comparison of metabolomic profiles obtained through LC-MS and NMR indicated high complementarity between these two platforms. The present findings demonstrated the complementary nature of NMR and LC/MS, implying that combination use of NMR and LC/MS facilitated a more comprehensive profiling of metabolite. 2017-09 Thesis https://ir.uitm.edu.my/id/eprint/38869/ https://ir.uitm.edu.my/id/eprint/38869/1/38869.pdf text en public masters Universiti Teknologi MARA Faculty of Pharmacy Ramasamy, Kalavathy (Prof. Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ramasamy, Kalavathy (Prof. Dr.)
topic	Diseases of the circulatory (Cardiovascular) system Diseases of the musculoskeletal system
spellingShingle	Diseases of the circulatory (Cardiovascular) system Diseases of the musculoskeletal system Enche Ady, Che Nor Adlia Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady
description	Alzheimer's disease (AD) is characterized by loss of memory and deterioration of cognitive function. To date, AD treatment remains a great challenge as pathogenesis of this disease is still poorly understood. Reliable AD biomarkers would therefore be of high relevance, not only to assist early detection but also to uncover potential drug targets that can be manipulated to slow down disease progression. This study compared metabolomic profiles of blood serum from AD patients to those of the non-demented controls. Qualitative metabolomic profiles of blood serum were acquired using the liquid chromatography/mass spectrometry quadrupole time-of-flight (LC-MSQTOF) and Nuclear Magnetic Resonance (NMR). The most important finding that emerged from this study is that lipids and amino acids are responsible for the metabolic changes linked to AD. The present study has revealed four LC-MS-derived metabolites [(N-(2- hydroxyethyl)palmita ide, N-(2-hydroxyethyl)icosanamide, dihydroceramide, Phyto sphingosine] and four NMR-derived metabolites (isoleucine, creatinine, VLDL, lipid) that might be implicated in AD pathogenesis. These metabolites panel may serve as potential biomarkers which are associated with neuronal P amyloid release, apoptosis, dysregulated energy metabolism and brain oxidative impairment that will lead to neuronal cell death. In terms of pathways, sphingolipid metabolism and biosynthesis of valine, leucine and isoleucine were found to be perturbed in AD. Comparison of metabolomic profiles obtained through LC-MS and NMR indicated high complementarity between these two platforms. The present findings demonstrated the complementary nature of NMR and LC/MS, implying that combination use of NMR and LC/MS facilitated a more comprehensive profiling of metabolite.
format	Thesis
qualification_level	Master's degree
author	Enche Ady, Che Nor Adlia
author_facet	Enche Ady, Che Nor Adlia
author_sort	Enche Ady, Che Nor Adlia
title	Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady
title_short	Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady
title_full	Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady
title_fullStr	Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady
title_full_unstemmed	Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady
title_sort	serum metabolic profiling for detection of alzheimer's disease / che nor adlia enche ady
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Pharmacy
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/38869/1/38869.pdf
_version_	1783734488573935616

Serum metabolic profiling for detection of Alzheimer's disease / Che Nor Adlia Enche Ady

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