Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman

Lycium barbarum or known as goji is a type of plant species with potential medicinal values. It derives from Solanaceae family and native to some areas of China. The present study was conducted to apply micropropagation technique on L. barbarum for local propagation and to assess the antioxidant act...

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Main Author:	Osman, Nurul Izzati
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Analytical chemistry QR Microbiology
Online Access:	https://ir.uitm.edu.my/id/eprint/39797/1/39797.pdf
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id	my-uitm-ir.39797
record_format	uketd_dc
spelling	my-uitm-ir.397972022-11-01T08:07:50Z Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman 2012 Osman, Nurul Izzati Analytical chemistry QR Microbiology Lycium barbarum or known as goji is a type of plant species with potential medicinal values. It derives from Solanaceae family and native to some areas of China. The present study was conducted to apply micropropagation technique on L. barbarum for local propagation and to assess the antioxidant activity by DPPH (diphenyl picryhydrazyl) assay. The best explant and corresponding treatment for micropropagation part were determined. In addition, the antioxidant activities of different ages (two to five month old) and plant parts (leaf, stem and root) of in vitro seedlings were also identified. Leaves and nodes were used as explants in micropropagation. For in vitro regeneration, the optimum combination was found in leaf explant treated with 0.5 mg/L NAA and 0.5 mg/L BAP in MS media. For callus induction, the treatment of 0.3 mg/L 2,4-D and 0.1 mg/L or 0.3 mg/L BAP in MS media were the optimal treatments in leaf explant while 0.1 mg/L BAP with either 0.3 mg/L or 0.5 mg/L 2,4-D in MS media were the optimal treatments in nodal explant. As for somatic embryogenesis, after a series of subculture on MS basal media, the treatment of 10 mg/L 2,4-D and 0.1 mg/L BAP in MS media during callus induction phase in leaf explant were identified to be the best combination. Meanwhile, from the DPPH assay, the methanolic extracts from leaf and stem of two month old seedlings were found to have the highest antioxidant activity with the EC₅₀ value of 0.08 mg/mL. The results revealed that L. barbarum has the potency to be excellently micropropagated and possesses an outstanding activity of antioxidant. 2012 Thesis https://ir.uitm.edu.my/id/eprint/39797/ https://ir.uitm.edu.my/id/eprint/39797/1/39797.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Plantation and Agrotechnology Awal, Asmah Jaafar Sidik, Norrizah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Awal, Asmah Jaafar Sidik, Norrizah
topic	Analytical chemistry QR Microbiology
spellingShingle	Analytical chemistry QR Microbiology Osman, Nurul Izzati Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman
description	Lycium barbarum or known as goji is a type of plant species with potential medicinal values. It derives from Solanaceae family and native to some areas of China. The present study was conducted to apply micropropagation technique on L. barbarum for local propagation and to assess the antioxidant activity by DPPH (diphenyl picryhydrazyl) assay. The best explant and corresponding treatment for micropropagation part were determined. In addition, the antioxidant activities of different ages (two to five month old) and plant parts (leaf, stem and root) of in vitro seedlings were also identified. Leaves and nodes were used as explants in micropropagation. For in vitro regeneration, the optimum combination was found in leaf explant treated with 0.5 mg/L NAA and 0.5 mg/L BAP in MS media. For callus induction, the treatment of 0.3 mg/L 2,4-D and 0.1 mg/L or 0.3 mg/L BAP in MS media were the optimal treatments in leaf explant while 0.1 mg/L BAP with either 0.3 mg/L or 0.5 mg/L 2,4-D in MS media were the optimal treatments in nodal explant. As for somatic embryogenesis, after a series of subculture on MS basal media, the treatment of 10 mg/L 2,4-D and 0.1 mg/L BAP in MS media during callus induction phase in leaf explant were identified to be the best combination. Meanwhile, from the DPPH assay, the methanolic extracts from leaf and stem of two month old seedlings were found to have the highest antioxidant activity with the EC₅₀ value of 0.08 mg/mL. The results revealed that L. barbarum has the potency to be excellently micropropagated and possesses an outstanding activity of antioxidant.
format	Thesis
qualification_level	Master's degree
author	Osman, Nurul Izzati
author_facet	Osman, Nurul Izzati
author_sort	Osman, Nurul Izzati
title	Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman
title_short	Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman
title_full	Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman
title_fullStr	Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman
title_full_unstemmed	Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman
title_sort	micropropagation and antioxidant screening of lycium barbarum l. (goji) / nurul izzati osman
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/39797/1/39797.pdf
_version_	1783734540955549696

Micropropagation and antioxidant screening of Lycium barbarum L. (goji) / Nurul Izzati Osman

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