Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir

P. betle leaves are known to have antimicrobial and antioxidant activities widely used as traditional medicine in Asian countries. This study investigated the antimicrobial activities of ethanolic and aqueous extract of P. betle against S. aureus (ATCC 25923) by determining the Minimum Inhibitory Co...

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Main Author:	Md Nasir, Nor Anis Nadhirah
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Microbial ecology
Online Access:	https://ir.uitm.edu.my/id/eprint/39828/1/39828.pdf
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spelling	my-uitm-ir.398282022-04-07T01:22:31Z Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir 2012-09 Md Nasir, Nor Anis Nadhirah Microbial ecology P. betle leaves are known to have antimicrobial and antioxidant activities widely used as traditional medicine in Asian countries. This study investigated the antimicrobial activities of ethanolic and aqueous extract of P. betle against S. aureus (ATCC 25923) by determining the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and its effects on the katA expression in S. aureus. The antioxidative activities were tested against H2O2 and oxidative burst in neutrophil. Hydrogen peroxide sensitivity test was performed to determine antioxidant of the ethanolic extract against H2O2. Catalase expression of S. aureuS was analyzed by the SDS-PAGE, NATIVE-PAGE analysis and further confirmed using ferric chloride and potassium ferricyanide. Its effect on oxidative burst was determined using chemiluminescence assay. Ethanolic extract of P.betle leaves showed significant higher antimicrobial activity compared to the aqueous extract where p<0.05. MICs values of ethanolic and aqueous extracts were 5mg/ml and 10mg/ml respectively. The hydrogen peroxide test showed the ethanolic extract able to detoxify hydrogen peroxide resulting in thirteen percent survival of cells. Interestingly, P. betle extract itself showed effective killing of S. aureus within thirty minutes. The RLU in the presence of ethanolic extract showed significant reduction (p<0.05) in oxidative burst by neutrophils. Catalase (58.3kDa) encoded by katA in S. aureus showed significant reduction after one hour treatment with ethanolic extract. The possible mechanism by which ethanolic extract of P.betle is inhibitory to S. aureus by downregulation of an important protein, catalase. Further work is required to quantitate the mRNA of katA expression following treatment with ethanolic of P. betle to confirm mechanism involved. 2012-09 Thesis https://ir.uitm.edu.my/id/eprint/39828/ https://ir.uitm.edu.my/id/eprint/39828/1/39828.pdf text en public masters Universiti Teknologi MARA Faculty of Health Science Mohamad Hussain, Roslinah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohamad Hussain, Roslinah (Dr.)
topic	Microbial ecology
spellingShingle	Microbial ecology Md Nasir, Nor Anis Nadhirah Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir
description	P. betle leaves are known to have antimicrobial and antioxidant activities widely used as traditional medicine in Asian countries. This study investigated the antimicrobial activities of ethanolic and aqueous extract of P. betle against S. aureus (ATCC 25923) by determining the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and its effects on the katA expression in S. aureus. The antioxidative activities were tested against H2O2 and oxidative burst in neutrophil. Hydrogen peroxide sensitivity test was performed to determine antioxidant of the ethanolic extract against H2O2. Catalase expression of S. aureuS was analyzed by the SDS-PAGE, NATIVE-PAGE analysis and further confirmed using ferric chloride and potassium ferricyanide. Its effect on oxidative burst was determined using chemiluminescence assay. Ethanolic extract of P.betle leaves showed significant higher antimicrobial activity compared to the aqueous extract where p<0.05. MICs values of ethanolic and aqueous extracts were 5mg/ml and 10mg/ml respectively. The hydrogen peroxide test showed the ethanolic extract able to detoxify hydrogen peroxide resulting in thirteen percent survival of cells. Interestingly, P. betle extract itself showed effective killing of S. aureus within thirty minutes. The RLU in the presence of ethanolic extract showed significant reduction (p<0.05) in oxidative burst by neutrophils. Catalase (58.3kDa) encoded by katA in S. aureus showed significant reduction after one hour treatment with ethanolic extract. The possible mechanism by which ethanolic extract of P.betle is inhibitory to S. aureus by downregulation of an important protein, catalase. Further work is required to quantitate the mRNA of katA expression following treatment with ethanolic of P. betle to confirm mechanism involved.
format	Thesis
qualification_level	Master's degree
author	Md Nasir, Nor Anis Nadhirah
author_facet	Md Nasir, Nor Anis Nadhirah
author_sort	Md Nasir, Nor Anis Nadhirah
title	Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir
title_short	Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir
title_full	Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir
title_fullStr	Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir
title_full_unstemmed	Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir
title_sort	antimicrobial and antioxidative effects of piper betle leaves extract on neutrophil survival and kata expression in staphylococcus aureus / nor anis nadhirah md nasir
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Health Science
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/39828/1/39828.pdf
_version_	1783734545373200384

Antimicrobial and antioxidative effects of Piper betle leaves extract on neutrophil survival and katA expression in Staphylococcus Aureus / Nor Anis Nadhirah Md Nasir

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