The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis

The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis

Ceiziella asiatica is a member of a tropical plant family. is known to have medicinal properties such as anti-oxidative stress and anti-inflammation. Since human periodontal ligament fibroblasts (HPLF) play a crucial role in the progression and healing of periodontal tissue inflammation. Therefore e...

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Main Author: Mohamed Younis, Awatef
Format: Thesis
Language:English
Published: 2011
Subjects:
RK Dentistry
Online Access:https://ir.uitm.edu.my/id/eprint/4829/1/TM_AWATEF%20MOHAMED%20YOUNIS%20DS%2011_5.pdf
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spelling my-uitm-ir.48292022-07-25T03:57:07Z The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis 2011 Mohamed Younis, Awatef RK Dentistry Ceiziella asiatica is a member of a tropical plant family. is known to have medicinal properties such as anti-oxidative stress and anti-inflammation. Since human periodontal ligament fibroblasts (HPLF) play a crucial role in the progression and healing of periodontal tissue inflammation. Therefore enhancing the role of the HPLF would result in promoting and accelerating the healing of periodontal tissues. The present study was designed to assess the effect of different concentrations of aqueous extract of Centella asiatica on the proliferation, cytoprotective activity and alkaline phosphatase activity of human periodontal ligament fibroblast in-vitro. Aqueous extract of Centella asiatica was prepared and concentrations of 2-40 .tg/ml were applied to the human periodontal ligament fibroblasts culture. The viability of human periodontal ligament fibroblast was investigated by MTT cell proliferation assay. The protective effect of Ceiitella asiatica against oxidative stress was done by measuring its effect on the 1-1 202 -induced cell damage. The differentiation of the periodontal ligament fibroblast cell was assessed by alkaline phosphatase (ALP) expression using Fluorescence Microplate Reader. The data were subjected to statistical analysis using ANOVA and Tukey's post hoc test. 2011 Thesis https://ir.uitm.edu.my/id/eprint/4829/ https://ir.uitm.edu.my/id/eprint/4829/1/TM_AWATEF%20MOHAMED%20YOUNIS%20DS%2011_5.pdf text en public masters Universiti Teknologi MARA Faculty of Dentistry
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
topic RK Dentistry
spellingShingle RK Dentistry
Mohamed Younis, Awatef
The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis
description Ceiziella asiatica is a member of a tropical plant family. is known to have medicinal properties such as anti-oxidative stress and anti-inflammation. Since human periodontal ligament fibroblasts (HPLF) play a crucial role in the progression and healing of periodontal tissue inflammation. Therefore enhancing the role of the HPLF would result in promoting and accelerating the healing of periodontal tissues. The present study was designed to assess the effect of different concentrations of aqueous extract of Centella asiatica on the proliferation, cytoprotective activity and alkaline phosphatase activity of human periodontal ligament fibroblast in-vitro. Aqueous extract of Centella asiatica was prepared and concentrations of 2-40 .tg/ml were applied to the human periodontal ligament fibroblasts culture. The viability of human periodontal ligament fibroblast was investigated by MTT cell proliferation assay. The protective effect of Ceiitella asiatica against oxidative stress was done by measuring its effect on the 1-1 202 -induced cell damage. The differentiation of the periodontal ligament fibroblast cell was assessed by alkaline phosphatase (ALP) expression using Fluorescence Microplate Reader. The data were subjected to statistical analysis using ANOVA and Tukey's post hoc test.
format Thesis
qualification_level Master's degree
author Mohamed Younis, Awatef
author_facet Mohamed Younis, Awatef
author_sort Mohamed Younis, Awatef
title The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis
title_short The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis
title_full The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis
title_fullStr The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis
title_full_unstemmed The effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / Awatef Mohamed Younis
title_sort effects of centella asia tica extract on human periodontal ligament fibroblasts cell line (2630) / awatef mohamed younis
granting_institution Universiti Teknologi MARA
granting_department Faculty of Dentistry
publishDate 2011
url https://ir.uitm.edu.my/id/eprint/4829/1/TM_AWATEF%20MOHAMED%20YOUNIS%20DS%2011_5.pdf
_version_ 1783733110904455168

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