Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali

Telomerase is an enzyme that catalyzes the addition of TTAGGG repeats to the end chromosomes, using its intrinsic RNA component as template. Telomerase activity is also shown to be expressed in most permanent cell lines and tumors. In this study, the oral rinse was used because it is a non-invasive...

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Main Author:	Che Ghazali, Norul Hajar
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Oral and dental medicine Pathology Diseases
Online Access:	https://ir.uitm.edu.my/id/eprint/48555/1/48555.pdf
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spelling	my-uitm-ir.485552022-07-07T07:31:46Z Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali 2013 Che Ghazali, Norul Hajar Oral and dental medicine. Pathology. Diseases Telomerase is an enzyme that catalyzes the addition of TTAGGG repeats to the end chromosomes, using its intrinsic RNA component as template. Telomerase activity is also shown to be expressed in most permanent cell lines and tumors. In this study, the oral rinse was used because it is a non-invasive method of collecting sample. In addition, the previous study has shown that telomerase activity can easily be detected by using FFPE tissue. There are several types of oral cancers, but around 90% are OSCC. The aim of this study was to investigate the feasibility detection of telomerase activity level in oral rinse and FFPE tissue sample from OSCC patients. Oral rinse collected from 20 normal subjects, 20 precancer and OSCC patients and 20 of FFPE OSCC tissues obtained from Oral Cancer Research Coordinating Centre of University Malaya (OCRCC). Protein extraction was performed by using protein isolation kit for oral rinse and protein FFPE extraction kit for FFPE samples. Telomerase activity level was confirmed by nonradioactive photometric enzyme immunoassay, ELISA using microplate reader and the absorbance value at wavelength 450 nm was measured for each sample. The 690 nm wavelength was used as the reference. Test absorbance values were reported as the A450 nm reading blank against the reference wavelength of A690 nm. It was positively detected that telomerase activity for pre cancer and patients with OSCC in oral rinse was 75% (15/20), while telomerase activity with OSCC patients in FFPE tissue samples was 95% (19/20), which was positively proven. In conclusion, the present work of employing non radioactive Telomerase PCR ELISA method has been successfully revealed FFPE tissues give higher telomerase activity in OSCC samples if compare with oral rinses. Thus, telomerase activity in oral rinses and FFPE tissues may be used as a diagnostic tool in early OSCC detection using this photometric enzyme immunoassay. 2013 Thesis https://ir.uitm.edu.my/id/eprint/48555/ https://ir.uitm.edu.my/id/eprint/48555/1/48555.pdf text en public masters Universiti Teknologi MARA Faculty of Dentistry Khor, Goot Heah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Khor, Goot Heah
topic	Oral and dental medicine Pathology Diseases
spellingShingle	Oral and dental medicine Pathology Diseases Che Ghazali, Norul Hajar Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali
description	Telomerase is an enzyme that catalyzes the addition of TTAGGG repeats to the end chromosomes, using its intrinsic RNA component as template. Telomerase activity is also shown to be expressed in most permanent cell lines and tumors. In this study, the oral rinse was used because it is a non-invasive method of collecting sample. In addition, the previous study has shown that telomerase activity can easily be detected by using FFPE tissue. There are several types of oral cancers, but around 90% are OSCC. The aim of this study was to investigate the feasibility detection of telomerase activity level in oral rinse and FFPE tissue sample from OSCC patients. Oral rinse collected from 20 normal subjects, 20 precancer and OSCC patients and 20 of FFPE OSCC tissues obtained from Oral Cancer Research Coordinating Centre of University Malaya (OCRCC). Protein extraction was performed by using protein isolation kit for oral rinse and protein FFPE extraction kit for FFPE samples. Telomerase activity level was confirmed by nonradioactive photometric enzyme immunoassay, ELISA using microplate reader and the absorbance value at wavelength 450 nm was measured for each sample. The 690 nm wavelength was used as the reference. Test absorbance values were reported as the A450 nm reading blank against the reference wavelength of A690 nm. It was positively detected that telomerase activity for pre cancer and patients with OSCC in oral rinse was 75% (15/20), while telomerase activity with OSCC patients in FFPE tissue samples was 95% (19/20), which was positively proven. In conclusion, the present work of employing non radioactive Telomerase PCR ELISA method has been successfully revealed FFPE tissues give higher telomerase activity in OSCC samples if compare with oral rinses. Thus, telomerase activity in oral rinses and FFPE tissues may be used as a diagnostic tool in early OSCC detection using this photometric enzyme immunoassay.
format	Thesis
qualification_level	Master's degree
author	Che Ghazali, Norul Hajar
author_facet	Che Ghazali, Norul Hajar
author_sort	Che Ghazali, Norul Hajar
title	Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali
title_short	Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali
title_full	Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali
title_fullStr	Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali
title_full_unstemmed	Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali
title_sort	detection of telomerase activity in oral squamous cell carcinoma patients by telomerase pcr elisa / norul hajar che ghazali
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Dentistry
publishDate	2013
url	https://ir.uitm.edu.my/id/eprint/48555/1/48555.pdf
_version_	1783734788812701696

Detection of telomerase activity in oral squamous cell carcinoma patients by telomerase PCR ELISA / Norul Hajar Che Ghazali

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