Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah
Microorganisms are ubiquitous, present almost everywhere. Various microorganisms can be found in the soil. Some of these microorganisms are capable of degrading petroleum hydrocarbons that contaminate the soil when oil tankers or pipes leaked. Petroleum consists of complex compound mixtures such as...
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my-uitm-ir.48852022-12-06T04:28:07Z Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah 2011 Abdullah, Nor Aishah Biodegradation QR Microbiology Microorganisms are ubiquitous, present almost everywhere. Various microorganisms can be found in the soil. Some of these microorganisms are capable of degrading petroleum hydrocarbons that contaminate the soil when oil tankers or pipes leaked. Petroleum consists of complex compound mixtures such as alkenes and aromatic hydrocarbon. These potential compounds could be eliminated by microbial remediation under suitable condition such as neutral pH, ambient temperature and presence of oxygen. Bioremediation of petroleum hydrocarbons is a complex process that depends on nature and amount of hydrocarbons present in the medium. It is a natural process that does not cause any harm to other organisms and also known as an alternative in cleaning oil spills because the cost is much lower than conventional methods. The purposes of this study are to screen the bacteria that are capable in degrading petroleum hydrocarbons, characterize them and identify the bacterial species. Soil samples were taken from workshops area at Jalan Mantap 25/126, Seksyen 25 Shah Alain. Samples were first enriched to increase the number of bacteria present. Spread plate method was used to isolate the bacteria. This was followed by streak plate method to obtain a single colony of each isolate. A total of 11 isolates were obtained and characterized by Gram staining and confirmed by database of bacteria species in Biolog system. After the screening process the strains undergone bioremediation to degrade total petroleum hydrocarbon in contaminated soils. The utilization of hydrocarbon after the treatment was detected by using GC-MS. All strains were capable to degrade tetradecane effectively compared to other compounds. From the findings, it shows that the strains of Pseudomonas aeruginosa, Acinetobacter baumannii and Pseudoinonas putida degraded hydrocarbon in soil significantly. The greatest bioremediation was performed by Pseudomonas aeruginosa with 41% of hydrocarbon followed by Pseudornonas putida with 30% of hydrocarbon and finally Acinetobacier baumannii with 29% of hydrocarbon after three months of incubation. These bacteria are found to be efficient to degrade petroleum hydrocarbons by utilizing them as their nutrients, carbon and energy sources. 2011 Thesis https://ir.uitm.edu.my/id/eprint/4885/ https://ir.uitm.edu.my/id/eprint/4885/3/4885.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Md Yusof, Farida Zuraina |
institution |
Universiti Teknologi MARA |
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UiTM Institutional Repository |
language |
English |
advisor |
Md Yusof, Farida Zuraina |
topic |
Biodegradation QR Microbiology |
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Biodegradation QR Microbiology Abdullah, Nor Aishah Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah |
description |
Microorganisms are ubiquitous, present almost everywhere. Various microorganisms can be found in the soil. Some of these microorganisms are capable of degrading petroleum hydrocarbons that contaminate the soil when oil tankers or pipes leaked. Petroleum consists of complex compound mixtures such as alkenes and aromatic hydrocarbon. These potential compounds could be eliminated by microbial remediation
under suitable condition such as neutral pH, ambient temperature and presence of oxygen. Bioremediation of petroleum hydrocarbons is a complex process that depends on nature and amount of hydrocarbons present in the medium. It is a natural process that does not cause any harm to other organisms and also known as an alternative in cleaning oil spills because the cost is much lower than conventional methods. The purposes of this study are to screen the bacteria that are capable in degrading petroleum hydrocarbons, characterize them and identify the bacterial species. Soil samples were taken from workshops area at Jalan Mantap 25/126, Seksyen 25 Shah Alain. Samples were first enriched to increase the number of bacteria present. Spread plate method was used to isolate the bacteria. This was followed by streak plate method to obtain a single colony of each isolate. A total of 11 isolates were obtained and characterized by Gram staining and confirmed by database of bacteria species in Biolog system. After the screening process the strains undergone bioremediation to degrade total petroleum hydrocarbon in contaminated soils. The utilization of hydrocarbon after the treatment was detected by using GC-MS. All strains were capable to degrade tetradecane effectively compared to other compounds. From the findings, it shows that the strains of Pseudomonas aeruginosa, Acinetobacter baumannii and Pseudoinonas putida degraded hydrocarbon in soil significantly. The greatest bioremediation was performed by Pseudomonas aeruginosa with 41% of hydrocarbon followed by Pseudornonas putida with 30% of hydrocarbon and finally Acinetobacier baumannii with 29% of
hydrocarbon after three months of incubation. These bacteria are found to be efficient to degrade petroleum hydrocarbons by utilizing them as their nutrients, carbon and energy sources. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Abdullah, Nor Aishah |
author_facet |
Abdullah, Nor Aishah |
author_sort |
Abdullah, Nor Aishah |
title |
Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah |
title_short |
Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah |
title_full |
Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah |
title_fullStr |
Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah |
title_full_unstemmed |
Screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / Nor Aishah Abdullah |
title_sort |
screening of bacteria from contaminated soil for the bioremediation of petroleum hydrocarbon / nor aishah abdullah |
granting_institution |
Universiti Teknologi MARA (UiTM) |
granting_department |
Faculty of Applied Sciences |
publishDate |
2011 |
url |
https://ir.uitm.edu.my/id/eprint/4885/3/4885.pdf |
_version_ |
1783733114569228288 |