A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim

The emerging resistance of pathogenic bacteria against the currently artificial antimicrobial agents demands the search of new antimicrobial agents. The use of medicinal plants as natural substitute of artificial antimicrobial agents is the paramount area of research to overcome the drug resistance...

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Main Author:	Abdul Halim, Ahmad Farizzuan
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Bacteria Antibacterial agents Materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/50068/1/50068.pdf
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id	my-uitm-ir.50068
record_format	uketd_dc
spelling	my-uitm-ir.500682021-09-06T03:13:18Z A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim 2017 Abdul Halim, Ahmad Farizzuan Bacteria Antibacterial agents Materia medica The emerging resistance of pathogenic bacteria against the currently artificial antimicrobial agents demands the search of new antimicrobial agents. The use of medicinal plants as natural substitute of artificial antimicrobial agents is the paramount area of research to overcome the drug resistance of pathogens. Therefore, this study was designed to investigate the antimicrobial activity from the combination of Lawsonia inermis and Zingiber zerumbet ethanolic extracts and their efficacy against selected skin pathogens. In the present study, antimicrobial activity from the combination of Lawsonia inermis and Zingiber zerumbet extracts are investigated against skin pathogens of four bacteria including two Gram positive (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram negative (Pseudomonas aeruginosa and Proteus mirabilis) using disc diffusion method. Ethanol was used as extraction solvent, while dimethyl sulfoxide (DMSO) and distilled water as dissolution solvents. The minimal concentration of antibiotic to kill or inhibit the microorganism was determined using microdilution broth method. Ethanolic extract from the combination of Lawsonia inermis and Zingiber zerumbet displayed noteworthy antimicrobial activity against both gram positive and gram negative bacteria. All tested bacteria are significant (P <0.05) when compared with single extract of Lawsonia inermis and Zingiber zerumbet. The minimum value of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for different bacterial strains ranged from 7.81mg/ml to 62.50mg/ml. In conclusion, it was proven that combination of both extracts yield better antimicrobial activity compared to the single extract. The use of combination from both extracts are of great significance as substitute antimicrobial agent in therapeutics. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50068/ https://ir.uitm.edu.my/id/eprint/50068/1/50068.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Rambely, Azlin Sham
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Rambely, Azlin Sham
topic	Bacteria Antibacterial agents Materia medica
spellingShingle	Bacteria Antibacterial agents Materia medica Abdul Halim, Ahmad Farizzuan A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim
description	The emerging resistance of pathogenic bacteria against the currently artificial antimicrobial agents demands the search of new antimicrobial agents. The use of medicinal plants as natural substitute of artificial antimicrobial agents is the paramount area of research to overcome the drug resistance of pathogens. Therefore, this study was designed to investigate the antimicrobial activity from the combination of Lawsonia inermis and Zingiber zerumbet ethanolic extracts and their efficacy against selected skin pathogens. In the present study, antimicrobial activity from the combination of Lawsonia inermis and Zingiber zerumbet extracts are investigated against skin pathogens of four bacteria including two Gram positive (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram negative (Pseudomonas aeruginosa and Proteus mirabilis) using disc diffusion method. Ethanol was used as extraction solvent, while dimethyl sulfoxide (DMSO) and distilled water as dissolution solvents. The minimal concentration of antibiotic to kill or inhibit the microorganism was determined using microdilution broth method. Ethanolic extract from the combination of Lawsonia inermis and Zingiber zerumbet displayed noteworthy antimicrobial activity against both gram positive and gram negative bacteria. All tested bacteria are significant (P <0.05) when compared with single extract of Lawsonia inermis and Zingiber zerumbet. The minimum value of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for different bacterial strains ranged from 7.81mg/ml to 62.50mg/ml. In conclusion, it was proven that combination of both extracts yield better antimicrobial activity compared to the single extract. The use of combination from both extracts are of great significance as substitute antimicrobial agent in therapeutics.
format	Thesis
qualification_level	Bachelor degree
author	Abdul Halim, Ahmad Farizzuan
author_facet	Abdul Halim, Ahmad Farizzuan
author_sort	Abdul Halim, Ahmad Farizzuan
title	A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim
title_short	A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim
title_full	A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim
title_fullStr	A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim
title_full_unstemmed	A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim
title_sort	study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / ahmad farizzuan abdul halim
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50068/1/50068.pdf
_version_	1783734817039319040

A study of antimicrobial activity against skin pathogens: combination of lawsonia inermis and zingiber zerumbet ethanolic extracts / Ahmad Farizzuan Abdul Halim

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