Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin

Formalin-fixed archive samples have been used as an alternative sources in a research study. Even though prolong formalin fixation causes the tissues undergo degradation due to formaldehyde modification reaction, the samples still valuable in genomic and histology study. However, this study using lu...

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Main Author:	Hussin, Hasliza
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Genetics Organic substances
Online Access:	https://ir.uitm.edu.my/id/eprint/50139/1/50139.pdf
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id	my-uitm-ir.50139
record_format	uketd_dc
spelling	my-uitm-ir.501392021-09-07T01:31:33Z Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin 2017 Hussin, Hasliza Genetics Chordates. Vertebrates Organic substances Formalin-fixed archive samples have been used as an alternative sources in a research study. Even though prolong formalin fixation causes the tissues undergo degradation due to formaldehyde modification reaction, the samples still valuable in genomic and histology study. However, this study using lung archive tissues still uncommon. Samples used are the lung tissue harvested from a rabbit fixed in 10 % formalin for seven and eight years. Tissue was processed for paraffin-embedded and stained with hematoxylin and eosin for histology assessment. RNA was extracted from samples, examined the integrity and concentration along with microRNA by using Agilent 2100 Bioanalyzer. Histology of all rabbit’s lung archive samples was comparable and has enough characteristic of lung tissue morphology. RNA with moderate to low integrity number (RIN) was isolated from archived samples with RIN 2.2 to 5.0 in 2010 samples and RIN 1.7 to 2.1 in 2009 samples. RNA concentration higher in 2009 sample (901 pg/µl) and lower in 2010 sample (41 pg/µl). Other than that, 27 to 56 percent of microRNA could be isolated from archive lung tissue with acceptable concentration (75.4 pg/µl to 169.6 pg/µl) and length (25 nt to 28 nt). In conclusion, our results demonstrated that formalin-fixed lung samples preserve better histology but due to the chemical modification RNA detect in moderate low integrity. However, RNA and miRNA concentration obtained was in an acceptable value. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50139/ https://ir.uitm.edu.my/id/eprint/50139/1/50139.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Mustakim, Maimunah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mustakim, Maimunah
topic	Genetics Genetics Organic substances
spellingShingle	Genetics Genetics Organic substances Hussin, Hasliza Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin
description	Formalin-fixed archive samples have been used as an alternative sources in a research study. Even though prolong formalin fixation causes the tissues undergo degradation due to formaldehyde modification reaction, the samples still valuable in genomic and histology study. However, this study using lung archive tissues still uncommon. Samples used are the lung tissue harvested from a rabbit fixed in 10 % formalin for seven and eight years. Tissue was processed for paraffin-embedded and stained with hematoxylin and eosin for histology assessment. RNA was extracted from samples, examined the integrity and concentration along with microRNA by using Agilent 2100 Bioanalyzer. Histology of all rabbit’s lung archive samples was comparable and has enough characteristic of lung tissue morphology. RNA with moderate to low integrity number (RIN) was isolated from archived samples with RIN 2.2 to 5.0 in 2010 samples and RIN 1.7 to 2.1 in 2009 samples. RNA concentration higher in 2009 sample (901 pg/µl) and lower in 2010 sample (41 pg/µl). Other than that, 27 to 56 percent of microRNA could be isolated from archive lung tissue with acceptable concentration (75.4 pg/µl to 169.6 pg/µl) and length (25 nt to 28 nt). In conclusion, our results demonstrated that formalin-fixed lung samples preserve better histology but due to the chemical modification RNA detect in moderate low integrity. However, RNA and miRNA concentration obtained was in an acceptable value.
format	Thesis
qualification_level	Bachelor degree
author	Hussin, Hasliza
author_facet	Hussin, Hasliza
author_sort	Hussin, Hasliza
title	Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin
title_short	Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin
title_full	Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin
title_fullStr	Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin
title_full_unstemmed	Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin
title_sort	histology and rna integrity of long-term formalin fixation storage rabbit's lung archive tissues / hasliza hussin
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50139/1/50139.pdf
_version_	1783734821210554368

Histology and RNA integrity of long-term formalin fixation storage rabbit's lung archive tissues / Hasliza Hussin

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