Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid

Andrographis paniculata or "Hempedu Bumi‟ is one of the herbal plants from the family Acanthaceae. The major phytochemical compound present in the leaves is Andrographolide which acst as an antifungal agent. Mold or fungal growth can be seen at General Research Laboratory at Faculty of Health S...

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Main Author:	Yazid, Izyan Nadhirah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Botanical chemistry Phytochemicals Drugs and their actions Materia medica
Online Access:	https://ir.uitm.edu.my/id/eprint/50142/1/50142.pdf
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spelling	my-uitm-ir.501422021-09-07T01:33:06Z Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid 2017 Yazid, Izyan Nadhirah Botanical chemistry. Phytochemicals Drugs and their actions Materia medica Andrographis paniculata or "Hempedu Bumi‟ is one of the herbal plants from the family Acanthaceae. The major phytochemical compound present in the leaves is Andrographolide which acst as an antifungal agent. Mold or fungal growth can be seen at General Research Laboratory at Faculty of Health Sciences UiTM Puncak Alam despite the treatment with chemicals. So, the aim of this study is to identify the isolated fungal and its effect with Andrographis paniculata leaves extraction. The isolated fungus was collected with sterile swab and culture on the SDA medium and air sampling method. The isolated fungus was identified by macroscopic method and observed based on the colony morphology. Microscopic method was also performed with slide culture method and use of Lactophenol Cotton Blue stain. The antifungal activity assay used of disk diffusion method with differences concentration of A. paniculata extract which were 1000, 500, 250, 125, 62.5, 31.25 mg/mL respectively. The final identification of isolated fungal is Cladosporium spp. was based on the macroscopic and microscopic identification and reference against published literature. The antifungal activity assay with disc diffusion method showed no inhibition zones for all difference concentrations of Andrographis paniculata extract that was tested. The positive control, Voriconazole (1µg) also showed no inhibition zone. The conclusion for this study is that the Andrographis paniculata leaves extract used in this study is not suitable for the antifungal assay due to prolonged storage. Further study can be done using of fresh extract and other test such as minimal inhibitory concentration and minimal fungicidal concentration for more accurate results. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50142/ https://ir.uitm.edu.my/id/eprint/50142/1/50142.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Mohamad Husain, Roslinah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohamad Husain, Roslinah
topic	Botanical chemistry Phytochemicals Drugs and their actions Materia medica
spellingShingle	Botanical chemistry Phytochemicals Drugs and their actions Materia medica Yazid, Izyan Nadhirah Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid
description	Andrographis paniculata or "Hempedu Bumi‟ is one of the herbal plants from the family Acanthaceae. The major phytochemical compound present in the leaves is Andrographolide which acst as an antifungal agent. Mold or fungal growth can be seen at General Research Laboratory at Faculty of Health Sciences UiTM Puncak Alam despite the treatment with chemicals. So, the aim of this study is to identify the isolated fungal and its effect with Andrographis paniculata leaves extraction. The isolated fungus was collected with sterile swab and culture on the SDA medium and air sampling method. The isolated fungus was identified by macroscopic method and observed based on the colony morphology. Microscopic method was also performed with slide culture method and use of Lactophenol Cotton Blue stain. The antifungal activity assay used of disk diffusion method with differences concentration of A. paniculata extract which were 1000, 500, 250, 125, 62.5, 31.25 mg/mL respectively. The final identification of isolated fungal is Cladosporium spp. was based on the macroscopic and microscopic identification and reference against published literature. The antifungal activity assay with disc diffusion method showed no inhibition zones for all difference concentrations of Andrographis paniculata extract that was tested. The positive control, Voriconazole (1µg) also showed no inhibition zone. The conclusion for this study is that the Andrographis paniculata leaves extract used in this study is not suitable for the antifungal assay due to prolonged storage. Further study can be done using of fresh extract and other test such as minimal inhibitory concentration and minimal fungicidal concentration for more accurate results.
format	Thesis
qualification_level	Bachelor degree
author	Yazid, Izyan Nadhirah
author_facet	Yazid, Izyan Nadhirah
author_sort	Yazid, Izyan Nadhirah
title	Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid
title_short	Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid
title_full	Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid
title_fullStr	Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid
title_full_unstemmed	Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid
title_sort	effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from general research laboratory, medical laboratory centre, faculty of health sciences, uitm puncak alam / izyan nadhirah yazid
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50142/1/50142.pdf
_version_	1783734821715968000

Effect of methanolic leaves extraction of androfraphis paniculata on fungus isolated from General Research Laboratory, Medical Laboratory Centre, Faculty of Health Sciences, UiTM Puncak Alam / Izyan Nadhirah Yazid

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