Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph

The rationale of using an artificial membrane feeder is to minimize the use of animals such as rats in direct host feeding method for mosquito blood feeding. This study focuses on testing vegetable oil, water, and glycerol to determine the best heating material to be used in the artificial membrane...

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Main Author:	Joseph, Mario
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Diptera (Flies) Infectious and parasitic diseases
Online Access:	https://ir.uitm.edu.my/id/eprint/50146/1/50146.pdf
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id	my-uitm-ir.50146
record_format	uketd_dc
spelling	my-uitm-ir.501462021-09-07T01:31:25Z Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph 2017 Joseph, Mario Diptera (Flies) Infectious and parasitic diseases Diet therapy. Dietary cookbooks The rationale of using an artificial membrane feeder is to minimize the use of animals such as rats in direct host feeding method for mosquito blood feeding. This study focuses on testing vegetable oil, water, and glycerol to determine the best heating material to be used in the artificial membrane feeder. This study also compares the feeding and oviposition rates of mosquitoes based on two different feeding method,s namely direct host feeding (DHF) and artificial feeding (AF). The heating materials were tested by pre-heating to ~80°C and ~50°C for heating the blood to 37±1°C and to maintain the temperature at 37±1°C, respectively. The mosquitoes were fed for 15 minutes for both methods and the eggs produced were calculated up to seven days after each feeding session. The feeding and oviposition rates were analyzed by using independent t test. The best heating material was glycerol and there is significant difference for both feeding rate (76.67±6.67% for DHF(r) and 26.67±6.67% for AF) and oviposition rate (43.13±1.59 eggs for DHF and 29.38±2.9 eggs for AF) for both methods where P<0.05, respectively. However, the eggs produced from AF method (235 eggs per 8 mosquitoes) were still sufficient for mosquito colony maintenance, and with increased sample size both parameters are expected to increase. In conclusion, the artificial membrane feeder proposed is sufficient to be used as alternative method in feeding mosquitoes for research purposes. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50146/ https://ir.uitm.edu.my/id/eprint/50146/1/50146.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Camalxaman, Siti Nazrina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Camalxaman, Siti Nazrina
topic	Diptera (Flies) Infectious and parasitic diseases Diptera (Flies)
spellingShingle	Diptera (Flies) Infectious and parasitic diseases Diptera (Flies) Joseph, Mario Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph
description	The rationale of using an artificial membrane feeder is to minimize the use of animals such as rats in direct host feeding method for mosquito blood feeding. This study focuses on testing vegetable oil, water, and glycerol to determine the best heating material to be used in the artificial membrane feeder. This study also compares the feeding and oviposition rates of mosquitoes based on two different feeding method,s namely direct host feeding (DHF) and artificial feeding (AF). The heating materials were tested by pre-heating to ~80°C and ~50°C for heating the blood to 37±1°C and to maintain the temperature at 37±1°C, respectively. The mosquitoes were fed for 15 minutes for both methods and the eggs produced were calculated up to seven days after each feeding session. The feeding and oviposition rates were analyzed by using independent t test. The best heating material was glycerol and there is significant difference for both feeding rate (76.67±6.67% for DHF(r) and 26.67±6.67% for AF) and oviposition rate (43.13±1.59 eggs for DHF and 29.38±2.9 eggs for AF) for both methods where P<0.05, respectively. However, the eggs produced from AF method (235 eggs per 8 mosquitoes) were still sufficient for mosquito colony maintenance, and with increased sample size both parameters are expected to increase. In conclusion, the artificial membrane feeder proposed is sufficient to be used as alternative method in feeding mosquitoes for research purposes.
format	Thesis
qualification_level	Bachelor degree
author	Joseph, Mario
author_facet	Joseph, Mario
author_sort	Joseph, Mario
title	Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph
title_short	Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph
title_full	Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph
title_fullStr	Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph
title_full_unstemmed	Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph
title_sort	comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / mario joseph
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50146/1/50146.pdf
_version_	1783734822207750144

Comparison of different heating materials for the development of an artificial feeding method for aedes albopictus / Mario Joseph

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