Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi
Staphylococcus epidermidis is gram positive bacteria and it is the most frequent cause of nosocomial infections due to indwelling medical devices and its dealings with human innate host. S.epidermidis is main virulence factor cause biofilm formation. Biofilm is a group of microorganism stick to each...
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| Language: | English |
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2017
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| Online Access: | https://ir.uitm.edu.my/id/eprint/50200/1/50200.pdf |
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my-uitm-ir.502002021-09-08T08:37:09Z Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi 2017 Azmi, Noramira Genetics QR Microbiology Clinical pathology. Laboratory technique Staphylococcus epidermidis is gram positive bacteria and it is the most frequent cause of nosocomial infections due to indwelling medical devices and its dealings with human innate host. S.epidermidis is main virulence factor cause biofilm formation. Biofilm is a group of microorganism stick to each other and adhere to cell surface. Biofilm development process in three steps which is initial adhesion, intercellular aggregation and accumulation, and final detachment. S.epidermidis controls the expression of clusters of virulence genes using the accessory gene regulator (Agr) quorum-sensing in biofilm formation. A sample Staphylococcus epidermidis bacterial DNA was extracted using boiling method. Real-Time Polymerase Chain Reaction (qPCR) will be useful for the detection of agr in Staphylococcus epidermidis. The application of real-time PCR proved a rapid and specific method for the detection agr Staphylococcus epidermidis in biofilm formation 2017 Thesis https://ir.uitm.edu.my/id/eprint/50200/ https://ir.uitm.edu.my/id/eprint/50200/1/50200.pdf text en public degree Universiti Teknologi Mara (Kampus Puncak Alam) Faculty of Health Sciences Mastuki, Mohd Fahmi |
| institution |
Universiti Teknologi MARA |
| collection |
UiTM Institutional Repository |
| language |
English |
| advisor |
Mastuki, Mohd Fahmi |
| topic |
Genetics QR Microbiology Genetics |
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Genetics QR Microbiology Genetics Azmi, Noramira Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi |
| description |
Staphylococcus epidermidis is gram positive bacteria and it is the most frequent cause of nosocomial infections due to indwelling medical devices and its dealings with human innate host. S.epidermidis is main virulence factor cause biofilm formation. Biofilm is a group of microorganism stick to each other and adhere to cell surface. Biofilm development process in three steps which is initial adhesion, intercellular aggregation and accumulation, and final detachment. S.epidermidis controls the expression of clusters of virulence genes using the accessory gene regulator (Agr) quorum-sensing in biofilm formation. A sample Staphylococcus epidermidis bacterial DNA was extracted using boiling method. Real-Time Polymerase Chain Reaction (qPCR) will be useful for the detection of agr in Staphylococcus epidermidis. The application of real-time PCR proved a rapid and specific method for the detection agr Staphylococcus epidermidis in biofilm formation |
| format |
Thesis |
| qualification_level |
Bachelor degree |
| author |
Azmi, Noramira |
| author_facet |
Azmi, Noramira |
| author_sort |
Azmi, Noramira |
| title |
Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi |
| title_short |
Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi |
| title_full |
Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi |
| title_fullStr |
Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi |
| title_full_unstemmed |
Molecular detection of accessory gene regulator (AGR) staphylococcus in biofilm formation / Noramira Azmi |
| title_sort |
molecular detection of accessory gene regulator (agr) staphylococcus in biofilm formation / noramira azmi |
| granting_institution |
Universiti Teknologi Mara (Kampus Puncak Alam) |
| granting_department |
Faculty of Health Sciences |
| publishDate |
2017 |
| url |
https://ir.uitm.edu.my/id/eprint/50200/1/50200.pdf |
| _version_ |
1783734824883716096 |