A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi

Background: Coriander (Coriandrum sativum) belongs to the Apiaceae family. It has been claimed to have antibacterial activity against Gram–positive and Gram-negative as well as fungi. This has lead to studying of phytochemical properties of Coriandrum sativum L aerial parts as an antimicrobial activ...

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Main Author:	Hazmi, Nur Diyanah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Botanical chemistry Phytochemicals QR Microbiology Vegetables
Online Access:	https://ir.uitm.edu.my/id/eprint/50230/1/50230.pdf
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id	my-uitm-ir.50230
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spelling	my-uitm-ir.502302021-09-09T02:09:04Z A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi 2017 Hazmi, Nur Diyanah Botanical chemistry. Phytochemicals QR Microbiology Vegetables Background: Coriander (Coriandrum sativum) belongs to the Apiaceae family. It has been claimed to have antibacterial activity against Gram–positive and Gram-negative as well as fungi. This has lead to studying of phytochemical properties of Coriandrum sativum L aerial parts as an antimicrobial activity against pathogenic microorganisms extracted by adding hexane as an additional steps for cleaning purpose and comparing the effectiveness as mentioned in previous study. Method: The plants of Coriandrum Sativum were purchased from local market in Selangor. The aerial parts were collected and washed with distilled water and dried in the hot air oven for 2 days, then made to powder form. The powdered samples with concentration (100g/500ml) was placed in 2 solvents which are methanol and hexane. As for hexane, the powder was soaked in 24 hours before being extracted by using methanol for the next day for 2 days. The extract from these solvents were soaked and evaporated under pressure by using rotary evaporator with the final concentration 1000mg/ml. Preliminary phytochemical screening and antimicrobial sensitivity testing (AST) were performed against pathogenic organism and compared the effectiveness. Results: The results of antimicrobial assay showed that methanolic extract of Coriandrum sativum L exhibited most antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis respectively. While for hexane-washed methanolic extract of Coriandrum sativum L have marked antibacterial activity against Bacillus cereus, Proteus mirabilis and Staphylococcus aureus. None of the extract exhibited antimicrobial activity against Acinetobacter baumannii. Comparison between methanolic extract and hexane-washed methanolic extract show significant result at same concentration. Conclusion: In conclusion, the leaves extract of Coriandrum sativum plant have marked antibacterial activity against many bacteria. From this study, it can be said that, additional steps by adding hexane as lipid removing purpose of Coriandrum sativum shows no significance changes. Nevertheless, both extractions showed a wide range of antibacterial activity and can be used for the medical purpose 2017 Thesis https://ir.uitm.edu.my/id/eprint/50230/ https://ir.uitm.edu.my/id/eprint/50230/1/50230.pdf text en public degree Universiti Teknologi Mara (Kampus Puncak Alam) Faculty of Health Sciences Rambely, Azlin Sham
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Rambely, Azlin Sham
topic	Botanical chemistry Phytochemicals QR Microbiology Vegetables
spellingShingle	Botanical chemistry Phytochemicals QR Microbiology Vegetables Hazmi, Nur Diyanah A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi
description	Background: Coriander (Coriandrum sativum) belongs to the Apiaceae family. It has been claimed to have antibacterial activity against Gram–positive and Gram-negative as well as fungi. This has lead to studying of phytochemical properties of Coriandrum sativum L aerial parts as an antimicrobial activity against pathogenic microorganisms extracted by adding hexane as an additional steps for cleaning purpose and comparing the effectiveness as mentioned in previous study. Method: The plants of Coriandrum Sativum were purchased from local market in Selangor. The aerial parts were collected and washed with distilled water and dried in the hot air oven for 2 days, then made to powder form. The powdered samples with concentration (100g/500ml) was placed in 2 solvents which are methanol and hexane. As for hexane, the powder was soaked in 24 hours before being extracted by using methanol for the next day for 2 days. The extract from these solvents were soaked and evaporated under pressure by using rotary evaporator with the final concentration 1000mg/ml. Preliminary phytochemical screening and antimicrobial sensitivity testing (AST) were performed against pathogenic organism and compared the effectiveness. Results: The results of antimicrobial assay showed that methanolic extract of Coriandrum sativum L exhibited most antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis respectively. While for hexane-washed methanolic extract of Coriandrum sativum L have marked antibacterial activity against Bacillus cereus, Proteus mirabilis and Staphylococcus aureus. None of the extract exhibited antimicrobial activity against Acinetobacter baumannii. Comparison between methanolic extract and hexane-washed methanolic extract show significant result at same concentration. Conclusion: In conclusion, the leaves extract of Coriandrum sativum plant have marked antibacterial activity against many bacteria. From this study, it can be said that, additional steps by adding hexane as lipid removing purpose of Coriandrum sativum shows no significance changes. Nevertheless, both extractions showed a wide range of antibacterial activity and can be used for the medical purpose
format	Thesis
qualification_level	Bachelor degree
author	Hazmi, Nur Diyanah
author_facet	Hazmi, Nur Diyanah
author_sort	Hazmi, Nur Diyanah
title	A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi
title_short	A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi
title_full	A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi
title_fullStr	A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi
title_full_unstemmed	A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi
title_sort	preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum l against pathogenic microorganisms / nur diyanah hazmi
granting_institution	Universiti Teknologi Mara (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50230/1/50230.pdf
_version_	1783734827301732352

A preliminary studies on phytochemicals and antimicrobial potential of coriandrum sativum L against pathogenic microorganisms / Nur Diyanah Hazmi

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