Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim

Watermelon or Citrullus lanatus (Thunb.) Matsum. And Nakai is a good source of phytonutrients and antioxidants such as Vitamin C, water soluble vitamins which is an essential micronutrient that have several important biological functions. Vitamin C as a potent antioxidant can eliminates reactive oxy...

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Main Author:	Abd Rahim, Nurarifah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Botanical chemistry Phytochemicals Materia medica Fruit and fruit culture
Online Access:	https://ir.uitm.edu.my/id/eprint/50238/1/50238.pdf
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id	my-uitm-ir.50238
record_format	uketd_dc
spelling	my-uitm-ir.502382021-09-09T01:45:48Z Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim 2017 Abd Rahim, Nurarifah Botanical chemistry. Phytochemicals Materia medica Fruit and fruit culture Watermelon or Citrullus lanatus (Thunb.) Matsum. And Nakai is a good source of phytonutrients and antioxidants such as Vitamin C, water soluble vitamins which is an essential micronutrient that have several important biological functions. Vitamin C as a potent antioxidant can eliminates reactive oxygen and nitrogen species and protective against oxidative damage. Due to lack of an enzyme human cannot synthesize ascorbic acid hence, it has to be supplemented through fruits such as watermelon. However, there are insufficient studies on ascorbic acid identification and quantification by using HPLC with better extraction method that can prevent ascorbic acid oxidation in local yellow watermelon. Therefore, this study was conducted to identify and quantify ascorbic acid compound in local yellow watermelon by using 3 Ortho-phosphoric and 8 Acetic acid extraction with 0.1 Ortho-phosphoric : Acetonitrile (95:5) mobile phase in reverse-phase High Performance Liquid Chromatography (HPLC). The retention time for the ascorbic acid in the chromatogram is 2.798 min for both standard and sample. Standard curves were linear over the concentration range from 100 to 500 µg/mL and the limits of detection and quantification were 0.47 and 1.43 µg/mL, respectively. The method was validated by doing spike recovery. The concentration of ascorbic acid in yellow flesh of local watermelon was found to be 19.05 µg/mL with percentage of 0.00002%. In conclusion, it can be stated that reverse-phase HPLC is the most preferred method to identify the presence of ascorbic acid. In addition, 3 Orthophosphoric and 8 Acetic acid in extraction method was a good method in order to prevent ascorbic acid oxidation. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50238/ https://ir.uitm.edu.my/id/eprint/50238/1/50238.pdf text en public degree Universiti Teknologi Mara (Kampus Puncak Alam) Faculty of Health Sciences Md Saad, Wan Mazlina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Md Saad, Wan Mazlina
topic	Botanical chemistry Phytochemicals Materia medica Fruit and fruit culture
spellingShingle	Botanical chemistry Phytochemicals Materia medica Fruit and fruit culture Abd Rahim, Nurarifah Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
description	Watermelon or Citrullus lanatus (Thunb.) Matsum. And Nakai is a good source of phytonutrients and antioxidants such as Vitamin C, water soluble vitamins which is an essential micronutrient that have several important biological functions. Vitamin C as a potent antioxidant can eliminates reactive oxygen and nitrogen species and protective against oxidative damage. Due to lack of an enzyme human cannot synthesize ascorbic acid hence, it has to be supplemented through fruits such as watermelon. However, there are insufficient studies on ascorbic acid identification and quantification by using HPLC with better extraction method that can prevent ascorbic acid oxidation in local yellow watermelon. Therefore, this study was conducted to identify and quantify ascorbic acid compound in local yellow watermelon by using 3 Ortho-phosphoric and 8 Acetic acid extraction with 0.1 Ortho-phosphoric : Acetonitrile (95:5) mobile phase in reverse-phase High Performance Liquid Chromatography (HPLC). The retention time for the ascorbic acid in the chromatogram is 2.798 min for both standard and sample. Standard curves were linear over the concentration range from 100 to 500 µg/mL and the limits of detection and quantification were 0.47 and 1.43 µg/mL, respectively. The method was validated by doing spike recovery. The concentration of ascorbic acid in yellow flesh of local watermelon was found to be 19.05 µg/mL with percentage of 0.00002%. In conclusion, it can be stated that reverse-phase HPLC is the most preferred method to identify the presence of ascorbic acid. In addition, 3 Orthophosphoric and 8 Acetic acid in extraction method was a good method in order to prevent ascorbic acid oxidation.
format	Thesis
qualification_level	Bachelor degree
author	Abd Rahim, Nurarifah
author_facet	Abd Rahim, Nurarifah
author_sort	Abd Rahim, Nurarifah
title	Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_short	Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_full	Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_fullStr	Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_full_unstemmed	Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_sort	detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (hplc) / nurarifah abd rahim
granting_institution	Universiti Teknologi Mara (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50238/1/50238.pdf
_version_	1783734828549537792

Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim

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