Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman

Centella asiatica, Moringae oleifera and Clitoria ternatea are widely used in Malaysian dishes, either to be eaten raw or as part of cooking ingredients. However, their potentials in preventing foodborne pathogens activity are not much explored. Therefore, the aim of this study was to investigate th...

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Main Author:	Othman, Nursuraya
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Clinical pathology Laboratory technique Materia medica Medicinal plants (Culture only)
Online Access:	https://ir.uitm.edu.my/id/eprint/50360/1/50360.pdf
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id	my-uitm-ir.50360
record_format	uketd_dc
spelling	my-uitm-ir.503602021-09-13T02:21:20Z Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman 2017 Othman, Nursuraya Clinical pathology. Laboratory technique Materia medica Medicinal plants (Culture only) Centella asiatica, Moringae oleifera and Clitoria ternatea are widely used in Malaysian dishes, either to be eaten raw or as part of cooking ingredients. However, their potentials in preventing foodborne pathogens activity are not much explored. Therefore, the aim of this study was to investigate the antimicrobial activities of Centella asiatica, Moringae oleifera and Clitoria ternatea extracts, and their synergistic effect against foodborne pathogens. In this study, the solvent extracts of the plants were tested against Listeria monocytogenes and Escherichia coli. Tests were done using disc diffusion method for antimicrobial sensitive testing (AST), broth micro dilution method for minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) by sub culturing the MIC sample in Mueller Hinton agar. The results showed that all plant extracts showed activity against both pathogens. Combination of all three types of extract against Listeria monocytogenes exhibited the largest inhibition zone. The AST result was supported by MIC and MBC results in which the combination of all three types of extract scored the lowest MIC and MBC concentration by 25%. In conclusion, Centella asiatica, Moringae oleifera and Clitoria ternatea extract were proven to have antimicrobial activity against the foodborne pathogens. However, the combination of all three types of extract did show some synergistic effect when tested against Listeria monocytogenes and Escherichia coli. However, the effectiveness of antimicrobial activity may vary in natural host since this study was performed in vitro entirely. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50360/ https://ir.uitm.edu.my/id/eprint/50360/1/50360.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Mustakim, Maimunah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mustakim, Maimunah
topic	Clinical pathology Laboratory technique Materia medica Medicinal plants (Culture only)
spellingShingle	Clinical pathology Laboratory technique Materia medica Medicinal plants (Culture only) Othman, Nursuraya Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman
description	Centella asiatica, Moringae oleifera and Clitoria ternatea are widely used in Malaysian dishes, either to be eaten raw or as part of cooking ingredients. However, their potentials in preventing foodborne pathogens activity are not much explored. Therefore, the aim of this study was to investigate the antimicrobial activities of Centella asiatica, Moringae oleifera and Clitoria ternatea extracts, and their synergistic effect against foodborne pathogens. In this study, the solvent extracts of the plants were tested against Listeria monocytogenes and Escherichia coli. Tests were done using disc diffusion method for antimicrobial sensitive testing (AST), broth micro dilution method for minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) by sub culturing the MIC sample in Mueller Hinton agar. The results showed that all plant extracts showed activity against both pathogens. Combination of all three types of extract against Listeria monocytogenes exhibited the largest inhibition zone. The AST result was supported by MIC and MBC results in which the combination of all three types of extract scored the lowest MIC and MBC concentration by 25%. In conclusion, Centella asiatica, Moringae oleifera and Clitoria ternatea extract were proven to have antimicrobial activity against the foodborne pathogens. However, the combination of all three types of extract did show some synergistic effect when tested against Listeria monocytogenes and Escherichia coli. However, the effectiveness of antimicrobial activity may vary in natural host since this study was performed in vitro entirely.
format	Thesis
qualification_level	Bachelor degree
author	Othman, Nursuraya
author_facet	Othman, Nursuraya
author_sort	Othman, Nursuraya
title	Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman
title_short	Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman
title_full	Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman
title_fullStr	Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman
title_full_unstemmed	Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman
title_sort	comparative study of antimicrobial activity between centella asiatica, moringae oleifera, and clitoria ternatea against selected foodborne pathogens / nursuraya othman
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50360/1/50360.pdf
_version_	1783734829579239424

Comparative study of antimicrobial activity between Centella asiatica, Moringae oleifera, and Clitoria ternatea against selected foodborne pathogens / Nursuraya Othman

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