Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim

Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim

Vitamin C or ascorbic acid is an essential water-soluble vitamin known for its antioxidant properties and act as a cofactor in numerous physiological reactions in the body. Being unable to be synthesized by human, ascorbic acid is consumed from most fruits and vegetables. Citrullus lanatus, a tropic...

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Main Author: Hashim, Nurul Nabilah Akmal
Format: Thesis
Language:English
Published: 2017
Subjects:
Analytical chemistry
Fruit and fruit culture
Online Access:https://ir.uitm.edu.my/id/eprint/50363/1/50363.pdf
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spelling my-uitm-ir.503632021-09-13T02:23:27Z Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim 2017 Hashim, Nurul Nabilah Akmal Analytical chemistry Fruit and fruit culture Vitamin C or ascorbic acid is an essential water-soluble vitamin known for its antioxidant properties and act as a cofactor in numerous physiological reactions in the body. Being unable to be synthesized by human, ascorbic acid is consumed from most fruits and vegetables. Citrullus lanatus, a tropical fruit with green smooth hard rind and juicy flesh provides a rich source of ascorbic acid. For this study, Thin Layer Chromatography (TLC) and reverse phase High Performance Liquid Chromatography (RP-HPLC) separation methods were used to determine ascorbic acid in the red flesh of Citrullus lanatus. Ascorbic acid was extracted from Citrullus lanatus red flesh juice using 3 % orthophosphoric acid and 8 % acetic acid by solid-liquid extraction method. Qualitative estimation was carried out by TLC method using several solvent systems and the optimized solvent was applied as the mobile phase in RP-HPLC. As for quantitative analysis, RP-HPLC was performed on a ZORBAX Eclipse XDB-C18 analytical column (250 x 4.6 mm , 5µm) using isocratic elution mobile phase 0.1 % orthophosphoric acid and acetonitrile (95:5, v/v) with flow rate of 1 mL/min, column temperature of 25 ˚C, injection volume of 20 µl and the wavelength detection of 254 nm. The limits of detection (LOD) and quantification (LOQ) were found as 0.47 µg/mL and 1.43 µg/mL respectively. Ascorbic acid was detected by RP-HPLC separation method at retention time of 2.796 minutes. High selectivity and sensitivity of RP-HPLC separation method compared to TLC allows ascorbic acid in red flesh of Citrullus lanatus to be detected and quantitated. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50363/ https://ir.uitm.edu.my/id/eprint/50363/1/50363.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Md Saad, Wan Mazlina
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Md Saad, Wan Mazlina
topic Analytical chemistry
Fruit and fruit culture
spellingShingle Analytical chemistry
Fruit and fruit culture
Hashim, Nurul Nabilah Akmal
Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim
description Vitamin C or ascorbic acid is an essential water-soluble vitamin known for its antioxidant properties and act as a cofactor in numerous physiological reactions in the body. Being unable to be synthesized by human, ascorbic acid is consumed from most fruits and vegetables. Citrullus lanatus, a tropical fruit with green smooth hard rind and juicy flesh provides a rich source of ascorbic acid. For this study, Thin Layer Chromatography (TLC) and reverse phase High Performance Liquid Chromatography (RP-HPLC) separation methods were used to determine ascorbic acid in the red flesh of Citrullus lanatus. Ascorbic acid was extracted from Citrullus lanatus red flesh juice using 3 % orthophosphoric acid and 8 % acetic acid by solid-liquid extraction method. Qualitative estimation was carried out by TLC method using several solvent systems and the optimized solvent was applied as the mobile phase in RP-HPLC. As for quantitative analysis, RP-HPLC was performed on a ZORBAX Eclipse XDB-C18 analytical column (250 x 4.6 mm , 5µm) using isocratic elution mobile phase 0.1 % orthophosphoric acid and acetonitrile (95:5, v/v) with flow rate of 1 mL/min, column temperature of 25 ˚C, injection volume of 20 µl and the wavelength detection of 254 nm. The limits of detection (LOD) and quantification (LOQ) were found as 0.47 µg/mL and 1.43 µg/mL respectively. Ascorbic acid was detected by RP-HPLC separation method at retention time of 2.796 minutes. High selectivity and sensitivity of RP-HPLC separation method compared to TLC allows ascorbic acid in red flesh of Citrullus lanatus to be detected and quantitated.
format Thesis
qualification_level Bachelor degree
author Hashim, Nurul Nabilah Akmal
author_facet Hashim, Nurul Nabilah Akmal
author_sort Hashim, Nurul Nabilah Akmal
title Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim
title_short Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim
title_full Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim
title_fullStr Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim
title_full_unstemmed Determination of ascorbic acid in citrullus lanatus red flesh juice by Thin Layer Chromatography (TLC) and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) / Nurul Nabilah Akmal Hashim
title_sort determination of ascorbic acid in citrullus lanatus red flesh juice by thin layer chromatography (tlc) and reverse phase high performance liquid chromatography (rp-hplc) / nurul nabilah akmal hashim
granting_institution Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department Faculty of Health Sciences
publishDate 2017
url https://ir.uitm.edu.my/id/eprint/50363/1/50363.pdf
_version_ 1783734830347845632

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