Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang

Acanthamoeba spp. is an opportunistic free-living amoeba that widely distributed in the environment and it has tendency to produce serious corneal infection that can result in permanent visual impairment or blindness known as chronic Acanthamoeba keratitis (CAK). Approximately 92% of Acanthamoeba ke...

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Main Author:	Amarang, Siti Aisyah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	Invertebrates RE Ophthalmology
Online Access:	https://ir.uitm.edu.my/id/eprint/50366/1/50366.pdf
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id	my-uitm-ir.50366
record_format	uketd_dc
spelling	my-uitm-ir.503662021-09-13T02:48:53Z Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang 2017 Amarang, Siti Aisyah Invertebrates RE Ophthalmology Acanthamoeba spp. is an opportunistic free-living amoeba that widely distributed in the environment and it has tendency to produce serious corneal infection that can result in permanent visual impairment or blindness known as chronic Acanthamoeba keratitis (CAK). Approximately 92% of Acanthamoeba keratitis patients were among contact lens wearers. Therefore, the usage of effective multipurpose contact lens solutions is currently recommended to prevent Acanthamoeba keratitis among contact lens wearers. This study was performed to determine the effectiveness of multipurpose contact lens solutions against Acanthamoeba spp. after 4, 6, 8 and 24 hours soaking time and also to classify the group of Acanthamoeba spp. based on their morphology using methylene blue stain. To determine the effectiveness of multipurpose contact lens solutions, two clinical specimens from keratitis patients were tested with five multipurpose contact lens solutions in which AOsept ® Plus, Oxysept ® , Opti-free ® Puremoist ® and Complete ® Revitalens ™ . The specimens were isolated in triplicate on nonnutrient agar seeded with heat-killed Escherichia coli suspension and been incubate at 30⁰C. Daily screening for the presence of Acanthamoeba spp. was done up to two weeks by using inverted microscope. The morphology of Acanthamoeba spp. was identified using methylene blue stain. Fisher‟s exact test was applied to determine the significance value of the results. The results revealed that both of hydrogen peroxide and non-hydrogen peroxide multipurpose contact lens solutions used in this study did not shows any anti-Acanthamoeba activity after 4, 6, 8 and 24 hours. This result is not significance since the p-value was higher than 0.001. Regarding to the morphology of Acanthamoeba spp., both of clinical isolates belongs to Group II of Acanthamoeba spp. As a conclusion, multipurpose contact lens solutions used in this study were not effective as anti-Acanthamoeba agents because of the active compounds in the contact lens solutions were insufficient. Thus, the addition of extra active compound in contact lens solutions such as propylene glycol for the disinfection of Acanthamoeba spp. should be developed in order to reduce the incidence rates of CAK among contact lens wearers. 2017 Thesis https://ir.uitm.edu.my/id/eprint/50366/ https://ir.uitm.edu.my/id/eprint/50366/1/50366.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Tengku Ahmad Basri, Tengku Shahrul Anuar
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Tengku Ahmad Basri, Tengku Shahrul Anuar
topic	Invertebrates RE Ophthalmology
spellingShingle	Invertebrates RE Ophthalmology Amarang, Siti Aisyah Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang
description	Acanthamoeba spp. is an opportunistic free-living amoeba that widely distributed in the environment and it has tendency to produce serious corneal infection that can result in permanent visual impairment or blindness known as chronic Acanthamoeba keratitis (CAK). Approximately 92% of Acanthamoeba keratitis patients were among contact lens wearers. Therefore, the usage of effective multipurpose contact lens solutions is currently recommended to prevent Acanthamoeba keratitis among contact lens wearers. This study was performed to determine the effectiveness of multipurpose contact lens solutions against Acanthamoeba spp. after 4, 6, 8 and 24 hours soaking time and also to classify the group of Acanthamoeba spp. based on their morphology using methylene blue stain. To determine the effectiveness of multipurpose contact lens solutions, two clinical specimens from keratitis patients were tested with five multipurpose contact lens solutions in which AOsept ® Plus, Oxysept ® , Opti-free ® Puremoist ® and Complete ® Revitalens ™ . The specimens were isolated in triplicate on nonnutrient agar seeded with heat-killed Escherichia coli suspension and been incubate at 30⁰C. Daily screening for the presence of Acanthamoeba spp. was done up to two weeks by using inverted microscope. The morphology of Acanthamoeba spp. was identified using methylene blue stain. Fisher‟s exact test was applied to determine the significance value of the results. The results revealed that both of hydrogen peroxide and non-hydrogen peroxide multipurpose contact lens solutions used in this study did not shows any anti-Acanthamoeba activity after 4, 6, 8 and 24 hours. This result is not significance since the p-value was higher than 0.001. Regarding to the morphology of Acanthamoeba spp., both of clinical isolates belongs to Group II of Acanthamoeba spp. As a conclusion, multipurpose contact lens solutions used in this study were not effective as anti-Acanthamoeba agents because of the active compounds in the contact lens solutions were insufficient. Thus, the addition of extra active compound in contact lens solutions such as propylene glycol for the disinfection of Acanthamoeba spp. should be developed in order to reduce the incidence rates of CAK among contact lens wearers.
format	Thesis
qualification_level	Bachelor degree
author	Amarang, Siti Aisyah
author_facet	Amarang, Siti Aisyah
author_sort	Amarang, Siti Aisyah
title	Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang
title_short	Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang
title_full	Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang
title_fullStr	Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang
title_full_unstemmed	Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang
title_sort	anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba spp. of keratitis patients / siti aisyah amarang
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2017
url	https://ir.uitm.edu.my/id/eprint/50366/1/50366.pdf
_version_	1783734831210823680

Anti-acanthamoeba activity of multipurpose contact lens solutions against acanthamoeba SPP. of keratitis patients / Siti Aisyah Amarang

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