Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan

Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan

Background: Few years back, high demands and limited supplies of hematoxylin dyes cause the price of the dyes increase and pose an apparent risk to rainforest ecology of producing country. Alternative to hematoxylin dyes determined to overcome the issue. Objective: determination of ability of aque...

Full description

Saved in:
Bibliographic Details
Main Author: Mohd Azlan, Siti Sarra Hazwani
Format: Thesis
Language:English
Published: 2017
Subjects:
Cytology
Online Access:https://ir.uitm.edu.my/id/eprint/50368/1/50368.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-uitm-ir.50368
record_format uketd_dc
spelling my-uitm-ir.503682021-09-13T03:09:06Z Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan 2017 Mohd Azlan, Siti Sarra Hazwani Cytology Flowers and flower culture. Ornamental plants Background: Few years back, high demands and limited supplies of hematoxylin dyes cause the price of the dyes increase and pose an apparent risk to rainforest ecology of producing country. Alternative to hematoxylin dyes determined to overcome the issue. Objective: determination of ability of aqueous extract of natural dye that derived from flower as a cytological dye for cell screening. Method: Fresh flower petals were desiccated, oven-dried, and undergone aqueous extraction to extract natural dyes. The natural dyes extracts were used in Pap Stain procedure by replacing the Hematoxylin dyes in staining buccal smear. The quality of the stain rated by 10 people and Weighted Kappa test was done using Statistical Package for the Social Sciences (SPSS) statistical analysis software. Results: The kappa values of Bougainvillea spectabilis extract with presence of mordant, Dianthus caryophyllus extract with presence of mordant, Hibiscus sabdariffa extract with presence of mordant, Costus woodsonii extract with and without presence of mordant are 0.442, 0.438, 0.538, 0.462 and 0.558 respectively, showed moderate strength of agreement. While Hibiscus sabdariffa extract without presence of mordant, Dianthus caryophyllus extract without presence of mordant and Rosa spp. extract with presence of mordant resulted 0.288, 0.243, 0.375, and 0.231 respectively showed fair strength in agreement. Lastly, Rosa spp. extract without presence of mordant showed poor strength in agreement as it‟s the kappa value is 0.000. Conclusion: All the aqueous extracts have the ability to stain nucleus. However, those extracts are incomparable to Pap Stain 2017 Thesis https://ir.uitm.edu.my/id/eprint/50368/ https://ir.uitm.edu.my/id/eprint/50368/1/50368.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Abu, Mohd Nazri
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Abu, Mohd Nazri
topic Cytology
Cytology
spellingShingle Cytology
Cytology
Mohd Azlan, Siti Sarra Hazwani
Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan
description Background: Few years back, high demands and limited supplies of hematoxylin dyes cause the price of the dyes increase and pose an apparent risk to rainforest ecology of producing country. Alternative to hematoxylin dyes determined to overcome the issue. Objective: determination of ability of aqueous extract of natural dye that derived from flower as a cytological dye for cell screening. Method: Fresh flower petals were desiccated, oven-dried, and undergone aqueous extraction to extract natural dyes. The natural dyes extracts were used in Pap Stain procedure by replacing the Hematoxylin dyes in staining buccal smear. The quality of the stain rated by 10 people and Weighted Kappa test was done using Statistical Package for the Social Sciences (SPSS) statistical analysis software. Results: The kappa values of Bougainvillea spectabilis extract with presence of mordant, Dianthus caryophyllus extract with presence of mordant, Hibiscus sabdariffa extract with presence of mordant, Costus woodsonii extract with and without presence of mordant are 0.442, 0.438, 0.538, 0.462 and 0.558 respectively, showed moderate strength of agreement. While Hibiscus sabdariffa extract without presence of mordant, Dianthus caryophyllus extract without presence of mordant and Rosa spp. extract with presence of mordant resulted 0.288, 0.243, 0.375, and 0.231 respectively showed fair strength in agreement. Lastly, Rosa spp. extract without presence of mordant showed poor strength in agreement as it‟s the kappa value is 0.000. Conclusion: All the aqueous extracts have the ability to stain nucleus. However, those extracts are incomparable to Pap Stain
format Thesis
qualification_level Bachelor degree
author Mohd Azlan, Siti Sarra Hazwani
author_facet Mohd Azlan, Siti Sarra Hazwani
author_sort Mohd Azlan, Siti Sarra Hazwani
title Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan
title_short Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan
title_full Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan
title_fullStr Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan
title_full_unstemmed Aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / Siti Sarra Hazwani Mohd Azlan
title_sort aqueous extracts of natural dyes originated from selected flower petals as alternative to cytological stain on buccal smear / siti sarra hazwani mohd azlan
granting_institution Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department Faculty of Health Sciences
publishDate 2017
url https://ir.uitm.edu.my/id/eprint/50368/1/50368.pdf
_version_ 1783734831724625920

Similar Items