Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik

Hibiscus sabdariffa is a flowering plant species of Malvaceae family which known as Roselle. It is used traditionally for many purposes such as hot and cold beverage that consumed by African, India, Mexico, Brazil, China, Iran and Malaysia. The H. sabdariffa is reported traditionally used as antisep...

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Main Author:	Peterik, Adeline Alui
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Botanical chemistry Phytochemicals Antibacterial agents
Online Access:	https://ir.uitm.edu.my/id/eprint/50492/1/50492.pdf
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id	my-uitm-ir.50492
record_format	uketd_dc
spelling	my-uitm-ir.504922021-09-15T01:45:23Z Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik 2019 Peterik, Adeline Alui Botanical chemistry. Phytochemicals Antibiotic therapy. Antibiotics Antibacterial agents Flowers and flower culture. Ornamental plants Hibiscus sabdariffa is a flowering plant species of Malvaceae family which known as Roselle. It is used traditionally for many purposes such as hot and cold beverage that consumed by African, India, Mexico, Brazil, China, Iran and Malaysia. The H. sabdariffa is reported traditionally used as antiseptic, aphrodisiac, astringent, cholagogue, demulcent, digestive, diuretic, emollient, purgative, refrigerant, sedative, stomachic and tonic. In many developing countries, the use of medical plants as traditional medicines is well known in rural areas. Therefore, a natural ingredient or plant which gives much less adverse effect is needed to replace the role of the commercial antibiotic in therapeutic antibiotic treatment. Hence, the aim of this study is to determine the potential of H. sabdariffa extract as natural antimicrobial agent against pathogenic bacteria as well as to detect its bioactive compounds. The H. sabdariffa powder was extracted with ethanol then performed antibacterial susceptibility testing (AST) against tested pathogenic bacteria using disc diffusion method. Then, Minimum Inhibitory Concentration (MIC) was done using broth microdilution method with microtiter plate. The preliminary phytochemical screening was done using different types of reagent for each bioactive compounds. The result for AST shows that the Staphylococcus epidermidis was most sensitive with the largest mean of inhibition zone (19.67 mm). It was followed by Staphylococcus aureus (15 mm), Pseudomonas aeruginosa (11 mm), and Escherichia coli (10.33 mm). All tested pathogenic bacteria had the same value of MIC, 7.82 mg/ml. the preliminary phytochemical screening revealed that H. sabdariffa contains alkaloids, glycosides, flavonoids and tannins. This finding showed that H. sabdariffa had antimicrobial activity against pathogenic bacteria and hold great promise as a natural product in antimicrobial agents. 2019 Thesis https://ir.uitm.edu.my/id/eprint/50492/ https://ir.uitm.edu.my/id/eprint/50492/1/50492.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Rambely, Azlin Sham
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Rambely, Azlin Sham
topic	Botanical chemistry Phytochemicals Botanical chemistry Phytochemicals Antibacterial agents Botanical chemistry Phytochemicals
spellingShingle	Botanical chemistry Phytochemicals Botanical chemistry Phytochemicals Antibacterial agents Botanical chemistry Phytochemicals Peterik, Adeline Alui Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik
description	Hibiscus sabdariffa is a flowering plant species of Malvaceae family which known as Roselle. It is used traditionally for many purposes such as hot and cold beverage that consumed by African, India, Mexico, Brazil, China, Iran and Malaysia. The H. sabdariffa is reported traditionally used as antiseptic, aphrodisiac, astringent, cholagogue, demulcent, digestive, diuretic, emollient, purgative, refrigerant, sedative, stomachic and tonic. In many developing countries, the use of medical plants as traditional medicines is well known in rural areas. Therefore, a natural ingredient or plant which gives much less adverse effect is needed to replace the role of the commercial antibiotic in therapeutic antibiotic treatment. Hence, the aim of this study is to determine the potential of H. sabdariffa extract as natural antimicrobial agent against pathogenic bacteria as well as to detect its bioactive compounds. The H. sabdariffa powder was extracted with ethanol then performed antibacterial susceptibility testing (AST) against tested pathogenic bacteria using disc diffusion method. Then, Minimum Inhibitory Concentration (MIC) was done using broth microdilution method with microtiter plate. The preliminary phytochemical screening was done using different types of reagent for each bioactive compounds. The result for AST shows that the Staphylococcus epidermidis was most sensitive with the largest mean of inhibition zone (19.67 mm). It was followed by Staphylococcus aureus (15 mm), Pseudomonas aeruginosa (11 mm), and Escherichia coli (10.33 mm). All tested pathogenic bacteria had the same value of MIC, 7.82 mg/ml. the preliminary phytochemical screening revealed that H. sabdariffa contains alkaloids, glycosides, flavonoids and tannins. This finding showed that H. sabdariffa had antimicrobial activity against pathogenic bacteria and hold great promise as a natural product in antimicrobial agents.
format	Thesis
qualification_level	Bachelor degree
author	Peterik, Adeline Alui
author_facet	Peterik, Adeline Alui
author_sort	Peterik, Adeline Alui
title	Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik
title_short	Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik
title_full	Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik
title_fullStr	Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik
title_full_unstemmed	Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik
title_sort	antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / adeline alui peterik
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50492/1/50492.pdf
_version_	1783734834770739200

Antimicrobial activity of roselle (hibiscus sabdariffa) calyx extract against pathogenic bacteria / Adeline Alui Peterik

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