Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah

Probiotics are living, and non-pathogenic microorganism, which gives health benefits to the human when administered in adequate amount. Lactobacillus spp. is one of the probiotic organism, which plays a major role in food fermentation and also take a part in therapeutic aspects of human health. Ther...

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主要作者:	Ishamshah, Ismah Firzanah
格式:	Thesis
语言:	English
出版:	2019
主题:	QR Microbiology Individual drugs and other agents SF Animal culture
在线阅读:	https://ir.uitm.edu.my/id/eprint/50580/1/50580.pdf
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id	my-uitm-ir.50580
record_format	uketd_dc
spelling	my-uitm-ir.505802021-09-21T01:45:46Z Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah 2019 Ishamshah, Ismah Firzanah QR Microbiology Individual drugs and other agents SF Animal culture Probiotics are living, and non-pathogenic microorganism, which gives health benefits to the human when administered in adequate amount. Lactobacillus spp. is one of the probiotic organism, which plays a major role in food fermentation and also take a part in therapeutic aspects of human health. There are various possible sources to isolate these bacteria. The purpose of this study is to isolate and identify the probiotic properties of the Lactobacillus spp. found in goat milk and probiotic powder by using biochemical and molecular technique. Isolates were phenotypically and genotypically characterized. The isolates were initially identified by their growth on MRS agar, gram staining and followed by testing their catalase and oxidase reactions. The isolates were then subjected to probiotic test, which includes bile salt tolerance test and antibiotic susceptibility testing. Further identifications were executed by molecular detection involving DNA extraction, PCR amplification of 16S rRNA gene. A total of 11 isolates designated as (M1, M2, M3, M4, M5, M6, M7, M8, S1, S2 and S3) expected to be Lactobacillus spp. was successfully identified based on the characteristics of growth on MRS agar and biochemistry reactions, Gram positive, catalase and oxidase negative. Amplification of 16S rRNA gene produced amplicon size of 566 base pairs (bp). Isolate M1 have least tolerance towards bile salt whereas isolate S3 are found to have slightly greater tolerance towards bile salt. Due to the ability to tolerate bile and an antibiotic susceptibility profiles, the isolates can be taken into consideration to be determined as probiotics bacteria. 2019 Thesis https://ir.uitm.edu.my/id/eprint/50580/ https://ir.uitm.edu.my/id/eprint/50580/1/50580.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Mustakim, Maimunah Alias, Rozila
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mustakim, Maimunah Alias, Rozila
topic	QR Microbiology Individual drugs and other agents SF Animal culture
spellingShingle	QR Microbiology Individual drugs and other agents SF Animal culture Ishamshah, Ismah Firzanah Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah
description	Probiotics are living, and non-pathogenic microorganism, which gives health benefits to the human when administered in adequate amount. Lactobacillus spp. is one of the probiotic organism, which plays a major role in food fermentation and also take a part in therapeutic aspects of human health. There are various possible sources to isolate these bacteria. The purpose of this study is to isolate and identify the probiotic properties of the Lactobacillus spp. found in goat milk and probiotic powder by using biochemical and molecular technique. Isolates were phenotypically and genotypically characterized. The isolates were initially identified by their growth on MRS agar, gram staining and followed by testing their catalase and oxidase reactions. The isolates were then subjected to probiotic test, which includes bile salt tolerance test and antibiotic susceptibility testing. Further identifications were executed by molecular detection involving DNA extraction, PCR amplification of 16S rRNA gene. A total of 11 isolates designated as (M1, M2, M3, M4, M5, M6, M7, M8, S1, S2 and S3) expected to be Lactobacillus spp. was successfully identified based on the characteristics of growth on MRS agar and biochemistry reactions, Gram positive, catalase and oxidase negative. Amplification of 16S rRNA gene produced amplicon size of 566 base pairs (bp). Isolate M1 have least tolerance towards bile salt whereas isolate S3 are found to have slightly greater tolerance towards bile salt. Due to the ability to tolerate bile and an antibiotic susceptibility profiles, the isolates can be taken into consideration to be determined as probiotics bacteria.
format	Thesis
qualification_level	Bachelor degree
author	Ishamshah, Ismah Firzanah
author_facet	Ishamshah, Ismah Firzanah
author_sort	Ishamshah, Ismah Firzanah
title	Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah
title_short	Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah
title_full	Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah
title_fullStr	Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah
title_full_unstemmed	Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah
title_sort	biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / ismah firzanah ishamshah
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50580/1/50580.pdf
_version_	1783734836037419008

Biochemical and molecular identification of lactobacillus spp. isolated from goat milk and probiotic powder / Ismah Firzanah Ishamshah

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