Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi

The main sugars that accumulate in watermelon consist of sucrose, glucose and fructose that determine sweetness of watermelon. The highest contribution of glucose and fructose in watermelon fruits is occurred during the early stages of fruit development, while sucrose proportion is detected when mat...

Full description

Saved in:

Bibliographic Details
Main Author:	Ramzi, Ainza Suzila
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Organic chemistry Analytical chemistry Fruit and fruit culture
Online Access:	https://ir.uitm.edu.my/id/eprint/50581/1/50581.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.50581
record_format	uketd_dc
spelling	my-uitm-ir.505812021-09-20T05:56:59Z Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi 2019 Ramzi, Ainza Suzila Organic chemistry Analytical chemistry Fruit and fruit culture The main sugars that accumulate in watermelon consist of sucrose, glucose and fructose that determine sweetness of watermelon. The highest contribution of glucose and fructose in watermelon fruits is occurred during the early stages of fruit development, while sucrose proportion is detected when maturity is achieved. So far, there is uncertainty in determining the maturity of watermelon and insufficient information of sucrose content in watermelon. The present study aims to determine and quantify the presence of sucrose in different parts of watermelon consist of flesh, rind and peel in the fresh juice and freeze dried samples by isocratic mode using reverse phase high performance liquid chromatography (RP-HPLC). The analysis was carried out using a mixture of acetonitrile (ACN) and ultrapure water (75:25, v/v) as mobile phase at isocratic elution conditions of 0-20 min. The chromatography separation was performed using ZORBAX NH2 column at flow rate of 1 ml/min and refractive index detection (RID). Sucrose standard was detected at 13.743 min of retention time. Linear standard curve at R2 = 0.9993 with very low limit of detection and limit of quantification at 0.00002 and 0.00007 mg/mL, respectively. In fresh juice samples, sucrose was detected at retention time of 13.754 minutes and 13.960 minutes with concentration of 7.86mg/mL and 0.61mg/mL in flesh and rind fresh juice, respectively. Meanwhile, in freeze dried samples, sucrose was only detected at retention time of 13.644 minutes with concentration of 0.96mg/mL in flesh. The isocratic mode RP-HPLC proved to be efficient method for determination and quantification of sucrose in fresh juice and freeze dried watermelon samples. 2019 Thesis https://ir.uitm.edu.my/id/eprint/50581/ https://ir.uitm.edu.my/id/eprint/50581/1/50581.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Md Saad, Mazlina
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Md Saad, Mazlina
topic	Organic chemistry Analytical chemistry Fruit and fruit culture
spellingShingle	Organic chemistry Analytical chemistry Fruit and fruit culture Ramzi, Ainza Suzila Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi
description	The main sugars that accumulate in watermelon consist of sucrose, glucose and fructose that determine sweetness of watermelon. The highest contribution of glucose and fructose in watermelon fruits is occurred during the early stages of fruit development, while sucrose proportion is detected when maturity is achieved. So far, there is uncertainty in determining the maturity of watermelon and insufficient information of sucrose content in watermelon. The present study aims to determine and quantify the presence of sucrose in different parts of watermelon consist of flesh, rind and peel in the fresh juice and freeze dried samples by isocratic mode using reverse phase high performance liquid chromatography (RP-HPLC). The analysis was carried out using a mixture of acetonitrile (ACN) and ultrapure water (75:25, v/v) as mobile phase at isocratic elution conditions of 0-20 min. The chromatography separation was performed using ZORBAX NH2 column at flow rate of 1 ml/min and refractive index detection (RID). Sucrose standard was detected at 13.743 min of retention time. Linear standard curve at R2 = 0.9993 with very low limit of detection and limit of quantification at 0.00002 and 0.00007 mg/mL, respectively. In fresh juice samples, sucrose was detected at retention time of 13.754 minutes and 13.960 minutes with concentration of 7.86mg/mL and 0.61mg/mL in flesh and rind fresh juice, respectively. Meanwhile, in freeze dried samples, sucrose was only detected at retention time of 13.644 minutes with concentration of 0.96mg/mL in flesh. The isocratic mode RP-HPLC proved to be efficient method for determination and quantification of sucrose in fresh juice and freeze dried watermelon samples.
format	Thesis
qualification_level	Bachelor degree
author	Ramzi, Ainza Suzila
author_facet	Ramzi, Ainza Suzila
author_sort	Ramzi, Ainza Suzila
title	Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi
title_short	Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi
title_full	Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi
title_fullStr	Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi
title_full_unstemmed	Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi
title_sort	determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (rp-hplc) / ainza suzila ramzi
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50581/1/50581.pdf
_version_	1783734836305854464

Determination and quantification of sucrose in different parts of watermelon (citrullus lanatus) by using reverse phase high performance liquid chromatography (RP-HPLC) / Ainza Suzila Ramzi

Similar Items