The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin

Alternanthera dentata and Lawsonia inermis leaves has been selected to substitute hematoxylin and Eosin Azure 50 in Papanicolaou stain which is a synthetic dye that has been widely used as a cytological stain. The purpose of the study is to evaluate the suitability of the mordants which are 2% of ph...

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Main Author:	Mohamad Tajudin, Anati
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Protoplasm Cytoplasm Field crops
Online Access:	https://ir.uitm.edu.my/id/eprint/50586/1/50586.pdf
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id	my-uitm-ir.50586
record_format	uketd_dc
spelling	my-uitm-ir.505862021-09-20T08:08:31Z The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin 2019 Mohamad Tajudin, Anati Protoplasm. Cytoplasm Field crops Alternanthera dentata and Lawsonia inermis leaves has been selected to substitute hematoxylin and Eosin Azure 50 in Papanicolaou stain which is a synthetic dye that has been widely used as a cytological stain. The purpose of the study is to evaluate the suitability of the mordants which are 2% of phosphotungstic acid and 3% of ferrous sulphate in enhancing the staining ability of Alternanthera dentata and Lawsonia inermis on nucleus and cytoplasm of buccal cell. From the study, it has been prove that ferrous sulphate can enhance the color uptake by the natural dye and having the ability to enhance the color uptake by the natural dyes by staining nucleus and the cytoplasm with its own color whereas phosphotungstic acid reduces the ability of the natural dyes to stain. Furthermore, the intermediate cells of the buccal smear are degenerated with an addition of 2% phosphotungstic acid which may due to the mordant’s high acidity level. A qualitative study has been done by selecting 10 volunteers to evaluate the staining color of the natural dyes with a comparison to Papanicolaou stain as a quality control. Whereas, a quantitative study were done by using MIPAR software, One-way ANOVA and Weighted Kappa Test. The statistical analysis shows that ferrous sulphate can enhance the color uptake but the color, specificity in determining superficial and intermediate cell and the color intensity are not comparable with the gold standard Papanicolaou stain. Thus, ferrous sulphate is the most suitable mordant that can increase the stainining efficacy of the Alternanthera dentata and Lawsonia inermis on buccal cells. 2019 Thesis https://ir.uitm.edu.my/id/eprint/50586/ https://ir.uitm.edu.my/id/eprint/50586/1/50586.pdf text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Health Sciences Abu, Mohd Nazri
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abu, Mohd Nazri
topic	Protoplasm Cytoplasm Field crops
spellingShingle	Protoplasm Cytoplasm Field crops Mohamad Tajudin, Anati The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin
description	Alternanthera dentata and Lawsonia inermis leaves has been selected to substitute hematoxylin and Eosin Azure 50 in Papanicolaou stain which is a synthetic dye that has been widely used as a cytological stain. The purpose of the study is to evaluate the suitability of the mordants which are 2% of phosphotungstic acid and 3% of ferrous sulphate in enhancing the staining ability of Alternanthera dentata and Lawsonia inermis on nucleus and cytoplasm of buccal cell. From the study, it has been prove that ferrous sulphate can enhance the color uptake by the natural dye and having the ability to enhance the color uptake by the natural dyes by staining nucleus and the cytoplasm with its own color whereas phosphotungstic acid reduces the ability of the natural dyes to stain. Furthermore, the intermediate cells of the buccal smear are degenerated with an addition of 2% phosphotungstic acid which may due to the mordant’s high acidity level. A qualitative study has been done by selecting 10 volunteers to evaluate the staining color of the natural dyes with a comparison to Papanicolaou stain as a quality control. Whereas, a quantitative study were done by using MIPAR software, One-way ANOVA and Weighted Kappa Test. The statistical analysis shows that ferrous sulphate can enhance the color uptake but the color, specificity in determining superficial and intermediate cell and the color intensity are not comparable with the gold standard Papanicolaou stain. Thus, ferrous sulphate is the most suitable mordant that can increase the stainining efficacy of the Alternanthera dentata and Lawsonia inermis on buccal cells.
format	Thesis
qualification_level	Bachelor degree
author	Mohamad Tajudin, Anati
author_facet	Mohamad Tajudin, Anati
author_sort	Mohamad Tajudin, Anati
title	The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin
title_short	The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin
title_full	The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin
title_fullStr	The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin
title_full_unstemmed	The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin
title_sort	efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / anati mohamad tajudin
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50586/1/50586.pdf
_version_	1783734837144715264

The efficacy of phosphotungstic acid and ferrous sulphate towards alternanthera dentata and lawsonia inermis in cytological staining for buccal cell screening / Anati Mohamad Tajudin

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