Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman

Throughout the years, the number of medically certified cancer keeps on increasing making cancer as the third leading cause of premature death in Malaysia. An antimitotic agent is a type of drug that inhibits cell growth by preventing mitosis and is used to cure cancer. In previous studies, Averrhoa...

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Main Author:	Mohd Sharulzaman, Siti Fatin Sobirah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Organic chemistry Fruit and fruit culture
Online Access:	https://ir.uitm.edu.my/id/eprint/50743/1/50743.pdf
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id	my-uitm-ir.50743
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spelling	my-uitm-ir.507432021-09-28T03:18:39Z Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman 2019-07 Mohd Sharulzaman, Siti Fatin Sobirah Organic chemistry Fruit and fruit culture Flowers and flower culture. Ornamental plants Throughout the years, the number of medically certified cancer keeps on increasing making cancer as the third leading cause of premature death in Malaysia. An antimitotic agent is a type of drug that inhibits cell growth by preventing mitosis and is used to cure cancer. In previous studies, Averrhoa bilimbi fruits have been proved to have many antioxidants compounds making it useful in many ways to treat disease. There are numerous studies on the phytochemical screening of A. bilimbi fruits. However, there is still no report concerning the antimitotic activity of A. bilimbi fruits. Hence, in present study, potential antimitotic activity of aqueous and methanol extract of A. bilimbi fruits in three different concentrations (20 mg/ml, 30 mg/ml, 40mg/ml) was measured in actively dividing Allium cepa root tip cells, and compared with the negative control (distilled water). The A. cepa roots were immersed in the aqueous and methanol extract of A. bilimbi fruits for 72 hours. One thousand cells per replicate were observed microscopically to calculate the percentage of mitotic index (MI). A one-way ANOVA showed that there was a significant decrease in the mitotic index (MI) of aqueous and methanol extract on the mitotic activity (p<0.001) of A. cepa roots. Between all groups, 40 mg/ml of methanol extract yields the lowest percentage of mitotic index (7.78%). The antimitotic activity of A. bilimbi may be due to its potential antioxidant properties particularly by the main role of phytochemicals. In conclusion, both aqueous and methanol extract shows antimitotic activity against actively dividing cells. This finding suggests that A. bilimbi fruits may be a new candidate for cancer treatment. Thus, further study needs to be conducted to determine the anticancer potential. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/50743/ https://ir.uitm.edu.my/id/eprint/50743/1/50743.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Abdul Hamid, Zed Zakari
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Abdul Hamid, Zed Zakari
topic	Organic chemistry Fruit and fruit culture Organic chemistry
spellingShingle	Organic chemistry Fruit and fruit culture Organic chemistry Mohd Sharulzaman, Siti Fatin Sobirah Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
description	Throughout the years, the number of medically certified cancer keeps on increasing making cancer as the third leading cause of premature death in Malaysia. An antimitotic agent is a type of drug that inhibits cell growth by preventing mitosis and is used to cure cancer. In previous studies, Averrhoa bilimbi fruits have been proved to have many antioxidants compounds making it useful in many ways to treat disease. There are numerous studies on the phytochemical screening of A. bilimbi fruits. However, there is still no report concerning the antimitotic activity of A. bilimbi fruits. Hence, in present study, potential antimitotic activity of aqueous and methanol extract of A. bilimbi fruits in three different concentrations (20 mg/ml, 30 mg/ml, 40mg/ml) was measured in actively dividing Allium cepa root tip cells, and compared with the negative control (distilled water). The A. cepa roots were immersed in the aqueous and methanol extract of A. bilimbi fruits for 72 hours. One thousand cells per replicate were observed microscopically to calculate the percentage of mitotic index (MI). A one-way ANOVA showed that there was a significant decrease in the mitotic index (MI) of aqueous and methanol extract on the mitotic activity (p<0.001) of A. cepa roots. Between all groups, 40 mg/ml of methanol extract yields the lowest percentage of mitotic index (7.78%). The antimitotic activity of A. bilimbi may be due to its potential antioxidant properties particularly by the main role of phytochemicals. In conclusion, both aqueous and methanol extract shows antimitotic activity against actively dividing cells. This finding suggests that A. bilimbi fruits may be a new candidate for cancer treatment. Thus, further study needs to be conducted to determine the anticancer potential.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Sharulzaman, Siti Fatin Sobirah
author_facet	Mohd Sharulzaman, Siti Fatin Sobirah
author_sort	Mohd Sharulzaman, Siti Fatin Sobirah
title	Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_short	Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_full	Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_fullStr	Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_full_unstemmed	Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_sort	antimitotic activity of averrhoa bilimbi l. (belimbing buluh) fruits extract in allium cepa root cells / siti fatin sobirah mohd sharulzaman
granting_institution	Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department	Faculty of Health Sciences
publishDate	2019
url	https://ir.uitm.edu.my/id/eprint/50743/1/50743.pdf
_version_	1783734839261790208

Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman

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