Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman

Throughout the years, the number of medically certified cancer keeps on increasing making cancer as the third leading cause of premature death in Malaysia. An antimitotic agent is a type of drug that inhibits cell growth by preventing mitosis and is used to cure cancer. In previous studies, Averrhoa...

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Main Author: Mohd Sharulzaman, Siti Fatin Sobirah
Format: Thesis
Language:English
Published: 2019
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Online Access:https://ir.uitm.edu.my/id/eprint/50743/1/50743.pdf
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spelling my-uitm-ir.507432021-09-28T03:18:39Z Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman 2019-07 Mohd Sharulzaman, Siti Fatin Sobirah Organic chemistry Fruit and fruit culture Flowers and flower culture. Ornamental plants Throughout the years, the number of medically certified cancer keeps on increasing making cancer as the third leading cause of premature death in Malaysia. An antimitotic agent is a type of drug that inhibits cell growth by preventing mitosis and is used to cure cancer. In previous studies, Averrhoa bilimbi fruits have been proved to have many antioxidants compounds making it useful in many ways to treat disease. There are numerous studies on the phytochemical screening of A. bilimbi fruits. However, there is still no report concerning the antimitotic activity of A. bilimbi fruits. Hence, in present study, potential antimitotic activity of aqueous and methanol extract of A. bilimbi fruits in three different concentrations (20 mg/ml, 30 mg/ml, 40mg/ml) was measured in actively dividing Allium cepa root tip cells, and compared with the negative control (distilled water). The A. cepa roots were immersed in the aqueous and methanol extract of A. bilimbi fruits for 72 hours. One thousand cells per replicate were observed microscopically to calculate the percentage of mitotic index (MI). A one-way ANOVA showed that there was a significant decrease in the mitotic index (MI) of aqueous and methanol extract on the mitotic activity (p<0.001) of A. cepa roots. Between all groups, 40 mg/ml of methanol extract yields the lowest percentage of mitotic index (7.78%). The antimitotic activity of A. bilimbi may be due to its potential antioxidant properties particularly by the main role of phytochemicals. In conclusion, both aqueous and methanol extract shows antimitotic activity against actively dividing cells. This finding suggests that A. bilimbi fruits may be a new candidate for cancer treatment. Thus, further study needs to be conducted to determine the anticancer potential. 2019-07 Thesis https://ir.uitm.edu.my/id/eprint/50743/ https://ir.uitm.edu.my/id/eprint/50743/1/50743.pdf text en public degree Universiti Teknologi MARA ( Kampus Puncak Alam ) Faculty of Health Sciences Abdul Hamid, Zed Zakari
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Abdul Hamid, Zed Zakari
topic Organic chemistry
Fruit and fruit culture
Organic chemistry
spellingShingle Organic chemistry
Fruit and fruit culture
Organic chemistry
Mohd Sharulzaman, Siti Fatin Sobirah
Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
description Throughout the years, the number of medically certified cancer keeps on increasing making cancer as the third leading cause of premature death in Malaysia. An antimitotic agent is a type of drug that inhibits cell growth by preventing mitosis and is used to cure cancer. In previous studies, Averrhoa bilimbi fruits have been proved to have many antioxidants compounds making it useful in many ways to treat disease. There are numerous studies on the phytochemical screening of A. bilimbi fruits. However, there is still no report concerning the antimitotic activity of A. bilimbi fruits. Hence, in present study, potential antimitotic activity of aqueous and methanol extract of A. bilimbi fruits in three different concentrations (20 mg/ml, 30 mg/ml, 40mg/ml) was measured in actively dividing Allium cepa root tip cells, and compared with the negative control (distilled water). The A. cepa roots were immersed in the aqueous and methanol extract of A. bilimbi fruits for 72 hours. One thousand cells per replicate were observed microscopically to calculate the percentage of mitotic index (MI). A one-way ANOVA showed that there was a significant decrease in the mitotic index (MI) of aqueous and methanol extract on the mitotic activity (p<0.001) of A. cepa roots. Between all groups, 40 mg/ml of methanol extract yields the lowest percentage of mitotic index (7.78%). The antimitotic activity of A. bilimbi may be due to its potential antioxidant properties particularly by the main role of phytochemicals. In conclusion, both aqueous and methanol extract shows antimitotic activity against actively dividing cells. This finding suggests that A. bilimbi fruits may be a new candidate for cancer treatment. Thus, further study needs to be conducted to determine the anticancer potential.
format Thesis
qualification_level Bachelor degree
author Mohd Sharulzaman, Siti Fatin Sobirah
author_facet Mohd Sharulzaman, Siti Fatin Sobirah
author_sort Mohd Sharulzaman, Siti Fatin Sobirah
title Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_short Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_full Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_fullStr Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_full_unstemmed Antimitotic activity of averrhoa bilimbi L. (belimbing buluh) fruits extract in allium cepa root cells / Siti Fatin Sobirah Mohd Sharulzaman
title_sort antimitotic activity of averrhoa bilimbi l. (belimbing buluh) fruits extract in allium cepa root cells / siti fatin sobirah mohd sharulzaman
granting_institution Universiti Teknologi MARA ( Kampus Puncak Alam )
granting_department Faculty of Health Sciences
publishDate 2019
url https://ir.uitm.edu.my/id/eprint/50743/1/50743.pdf
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